Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures
The biosensing of bacterial pathogens is of a high priority. Electrochemical biosensors are an important future tool for rapid bacteria detection. A monolayer of bacterial-binding peptides can serve as a recognition layer in such detection devices. Here, we explore the potential of random peptide mi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/2/561 |
| _version_ | 1797437379952246784 |
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| author | Tal Stern Bauer Ravit Yakobi Mattan Hurevich Shlomo Yitzchaik Zvi Hayouka |
| author_facet | Tal Stern Bauer Ravit Yakobi Mattan Hurevich Shlomo Yitzchaik Zvi Hayouka |
| author_sort | Tal Stern Bauer |
| collection | DOAJ |
| description | The biosensing of bacterial pathogens is of a high priority. Electrochemical biosensors are an important future tool for rapid bacteria detection. A monolayer of bacterial-binding peptides can serve as a recognition layer in such detection devices. Here, we explore the potential of random peptide mixtures (RPMs) composed of phenylalanine and lysine in random sequences and of controlled length, to form a monolayer that can be utilized for sensing. RPMs were found to assemble in a thin and diluted layer that attracts various bacteria. Faradaic electrochemical impedance spectroscopy was used with modified gold electrodes to measure the charge-transfer resistance (R<sub>CT</sub>) caused due to the binding of bacteria to RPMs. <i>Pseudomonas aeruginosa</i> was found to cause the most prominent increase in R<sub>CT</sub> compared to other model bacteria. We show that the combination of highly accessible antimicrobial RPMs and electrochemical analysis can be used to generate a new promising line of bacterial biosensors. |
| first_indexed | 2024-03-09T11:19:22Z |
| format | Article |
| id | doaj.art-9def1149d85d4e23b72e7a36aa600747 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T11:19:22Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9def1149d85d4e23b72e7a36aa6007472023-12-01T00:23:41ZengMDPI AGSensors1424-82202023-01-0123256110.3390/s23020561Impedimetric Bacterial Detection Using Random Antimicrobial Peptide MixturesTal Stern Bauer0Ravit Yakobi1Mattan Hurevich2Shlomo Yitzchaik3Zvi Hayouka4Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, IsraelInstitute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, IsraelInstitute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, IsraelInstitute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, IsraelInstitute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, IsraelThe biosensing of bacterial pathogens is of a high priority. Electrochemical biosensors are an important future tool for rapid bacteria detection. A monolayer of bacterial-binding peptides can serve as a recognition layer in such detection devices. Here, we explore the potential of random peptide mixtures (RPMs) composed of phenylalanine and lysine in random sequences and of controlled length, to form a monolayer that can be utilized for sensing. RPMs were found to assemble in a thin and diluted layer that attracts various bacteria. Faradaic electrochemical impedance spectroscopy was used with modified gold electrodes to measure the charge-transfer resistance (R<sub>CT</sub>) caused due to the binding of bacteria to RPMs. <i>Pseudomonas aeruginosa</i> was found to cause the most prominent increase in R<sub>CT</sub> compared to other model bacteria. We show that the combination of highly accessible antimicrobial RPMs and electrochemical analysis can be used to generate a new promising line of bacterial biosensors.https://www.mdpi.com/1424-8220/23/2/561bacteriarandom peptide mixturesantimicrobial peptideselectrochemical impedance spectroscopyself-assembly monolayer |
| spellingShingle | Tal Stern Bauer Ravit Yakobi Mattan Hurevich Shlomo Yitzchaik Zvi Hayouka Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures Sensors bacteria random peptide mixtures antimicrobial peptides electrochemical impedance spectroscopy self-assembly monolayer |
| title | Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures |
| title_full | Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures |
| title_fullStr | Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures |
| title_full_unstemmed | Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures |
| title_short | Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures |
| title_sort | impedimetric bacterial detection using random antimicrobial peptide mixtures |
| topic | bacteria random peptide mixtures antimicrobial peptides electrochemical impedance spectroscopy self-assembly monolayer |
| url | https://www.mdpi.com/1424-8220/23/2/561 |
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