From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm
Bacterial cellulose (BC) has recently been the subject of a considerable amount of research, not only for its environmentally friendly biosynthesis, but also for its high potential in areas such as biomedicine or biomaterials. A symbiotic relationship between a photosynthetic microalga, <i>Chl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/12/14/2275 |
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| author | Vítor Nóbrega Marisa Faria Antera Quintana Manfred Kaufmann Artur Ferreira Nereida Cordeiro |
| author_facet | Vítor Nóbrega Marisa Faria Antera Quintana Manfred Kaufmann Artur Ferreira Nereida Cordeiro |
| author_sort | Vítor Nóbrega |
| collection | DOAJ |
| description | Bacterial cellulose (BC) has recently been the subject of a considerable amount of research, not only for its environmentally friendly biosynthesis, but also for its high potential in areas such as biomedicine or biomaterials. A symbiotic relationship between a photosynthetic microalga, <i>Chlamydomonas debaryana</i>, and a cellulose producer bacterium, <i>Komagataeibacter saccharivorans</i>, was established in order to obtain a viable and active biofilm. The effect of the growth media composition ratio on the produced living material was investigated, as well as the microalgae biomass quantity, temperature, and incubation time. The optimal temperature for higher symbiotic biofilm production was 30 °C with an incubation period of 14 days. The high microalgae presence, 0.75% <i>w/v</i>, and 60:40 HS:BG-11 medium (<i>v/v</i>) induced a biofilm microalgae incorporation rate of 85%. The obtained results report, for the first time, a successful symbiotic interaction developed in situ between an alkaline photosynthetic microalga and an acetic acid bacterium. These results are promising and open a new window to BC living biofilm applications in medical fields that have not yet been explored. |
| first_indexed | 2024-04-12T22:52:47Z |
| format | Article |
| id | doaj.art-9df08747495343908cc1823575af1a01 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-04-12T22:52:47Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-9df08747495343908cc1823575af1a012022-12-22T03:13:18ZengMDPI AGMaterials1996-19442019-07-011214227510.3390/ma12142275ma12142275From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic BiofilmVítor Nóbrega0Marisa Faria1Antera Quintana2Manfred Kaufmann3Artur Ferreira4Nereida Cordeiro5LB3, Faculty of Science and Engineering, University of Madeira, 9000-390 Funchal, PortugalLB3, Faculty of Science and Engineering, University of Madeira, 9000-390 Funchal, PortugalBanco Español de Algas, Instituto de Oceanografía y Cambio Global (IOCAG), Universidad de Las Palmas de Gran Canaria, 35214 Telde, SpainMarine Biology Station of Funchal, Faculty of Life Sciences, University of Madeira, 9000-107 Funchal, PortugalCICECO, Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalLB3, Faculty of Science and Engineering, University of Madeira, 9000-390 Funchal, PortugalBacterial cellulose (BC) has recently been the subject of a considerable amount of research, not only for its environmentally friendly biosynthesis, but also for its high potential in areas such as biomedicine or biomaterials. A symbiotic relationship between a photosynthetic microalga, <i>Chlamydomonas debaryana</i>, and a cellulose producer bacterium, <i>Komagataeibacter saccharivorans</i>, was established in order to obtain a viable and active biofilm. The effect of the growth media composition ratio on the produced living material was investigated, as well as the microalgae biomass quantity, temperature, and incubation time. The optimal temperature for higher symbiotic biofilm production was 30 °C with an incubation period of 14 days. The high microalgae presence, 0.75% <i>w/v</i>, and 60:40 HS:BG-11 medium (<i>v/v</i>) induced a biofilm microalgae incorporation rate of 85%. The obtained results report, for the first time, a successful symbiotic interaction developed in situ between an alkaline photosynthetic microalga and an acetic acid bacterium. These results are promising and open a new window to BC living biofilm applications in medical fields that have not yet been explored.https://www.mdpi.com/1996-1944/12/14/2275bacterial cellulosemicroalgaeliving biofilm<i>Chlamydomonas debaryana</i><i>Komagataeibacter saccharivorans</i>symbioses |
| spellingShingle | Vítor Nóbrega Marisa Faria Antera Quintana Manfred Kaufmann Artur Ferreira Nereida Cordeiro From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm Materials bacterial cellulose microalgae living biofilm <i>Chlamydomonas debaryana</i> <i>Komagataeibacter saccharivorans</i> symbioses |
| title | From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm |
| title_full | From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm |
| title_fullStr | From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm |
| title_full_unstemmed | From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm |
| title_short | From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm |
| title_sort | from a basic microalga and an acetic acid bacterium cellulose producer to a living symbiotic biofilm |
| topic | bacterial cellulose microalgae living biofilm <i>Chlamydomonas debaryana</i> <i>Komagataeibacter saccharivorans</i> symbioses |
| url | https://www.mdpi.com/1996-1944/12/14/2275 |
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