Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plants
The demand for the production of herbal extracts for cosmetics, food, and health supplements, known as plant-based medicine, is rising globally. Incorporating herbal extracts could help to create higher value products due to the functional properties of bioactive compounds. Because the phytochemical...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.980066/full |
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| author | Hira Zahoor Kwanrutai Watchaputi Janejira Hata Wachirachai Pabuprapap Apichart Suksamrarn Lee Suan Chua Nitnipa Soontorngun |
| author_facet | Hira Zahoor Kwanrutai Watchaputi Janejira Hata Wachirachai Pabuprapap Apichart Suksamrarn Lee Suan Chua Nitnipa Soontorngun |
| author_sort | Hira Zahoor |
| collection | DOAJ |
| description | The demand for the production of herbal extracts for cosmetics, food, and health supplements, known as plant-based medicine, is rising globally. Incorporating herbal extracts could help to create higher value products due to the functional properties of bioactive compounds. Because the phytochemical composition could vary depending on the processing methods, a simple bioassay of herbal bioactive compounds is an important screening method for the purposes of functional characterization and quality assurance. As a simplified eukaryotic model, yeast serves as a versatile tool to examine functional property of bioactive compounds and to gain better understanding of fundamental cellular processes, because they share similarities with the processes in humans. In fact, aging is a well-conserved phenomenon between yeast and humans, making yeast a powerful genetic tool to examine functional properties of key compounds obtained from plant extracts. This study aimed to apply a well-established model yeast, Saccharomyces cerevisiae, to examine the antioxidant and anti-aging potential of flavonoids, extracted from medicinal plants, and to gain insight into yeast cell adaptation to oxidative stress. Some natural quercetin analogs, including morin, kaempferol, aromadendrin, and steppogenin, protected yeast cells against oxidative stress induced by acetic acid, as shown by decreased cell sensitivity. There was also a reduction in intracellular reactive oxygen species following acetic acid treatment. Using the chronological aging assay, quercetin, morin, and steppogenin could extend the lifespan of wild-type S. cerevisiae by 15%–25%. Consistent with the fact that oxidative stress is a key factor to aging, acetic acid resistance was associated with increased gene expression of TOR1, which encodes a key growth signaling kinase, and MSN2 and MSN4, which encode stress-responsive transcription factors. The addition of the antioxidant morin could counteract this increased expression, suggesting a possible modulatory role in cell signaling and the stress response of yeast. Therefore, yeast represents a versatile model organism and rapid screening tool to discover potentially rejuvenescent molecules with anti-aging and anti-oxidant potential from natural resources and to advance knowledge in the molecular study of stress and aging. |
| first_indexed | 2024-04-11T13:20:27Z |
| format | Article |
| id | doaj.art-9df13aed99734ea58263be47f959f1f8 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T13:20:27Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-9df13aed99734ea58263be47f959f1f82022-12-22T04:22:14ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-09-011310.3389/fphar.2022.980066980066Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plantsHira Zahoor0Kwanrutai Watchaputi1Janejira Hata2Wachirachai Pabuprapap3Apichart Suksamrarn4Lee Suan Chua5Nitnipa Soontorngun6Gene Technology Laboratory, Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, ThailandGene Technology Laboratory, Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, ThailandDepartment of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Sciences, Ramkhamhaeng University, Bangkok, ThailandDepartment of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Sciences, Ramkhamhaeng University, Bangkok, ThailandDepartment of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Sciences, Ramkhamhaeng University, Bangkok, ThailandMetabolites Profiling Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia, Johor Bahru, Johor, MalaysiaGene Technology Laboratory, Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, ThailandThe demand for the production of herbal extracts for cosmetics, food, and health supplements, known as plant-based medicine, is rising globally. Incorporating herbal extracts could help to create higher value products due to the functional properties of bioactive compounds. Because the phytochemical composition could vary depending on the processing methods, a simple bioassay of herbal bioactive compounds is an important screening method for the purposes of functional characterization and quality assurance. As a simplified eukaryotic model, yeast serves as a versatile tool to examine functional property of bioactive compounds and to gain better understanding of fundamental cellular processes, because they share similarities with the processes in humans. In fact, aging is a well-conserved phenomenon between yeast and humans, making yeast a powerful genetic tool to examine functional properties of key compounds obtained from plant extracts. This study aimed to apply a well-established model yeast, Saccharomyces cerevisiae, to examine the antioxidant and anti-aging potential of flavonoids, extracted from medicinal plants, and to gain insight into yeast cell adaptation to oxidative stress. Some natural quercetin analogs, including morin, kaempferol, aromadendrin, and steppogenin, protected yeast cells against oxidative stress induced by acetic acid, as shown by decreased cell sensitivity. There was also a reduction in intracellular reactive oxygen species following acetic acid treatment. Using the chronological aging assay, quercetin, morin, and steppogenin could extend the lifespan of wild-type S. cerevisiae by 15%–25%. Consistent with the fact that oxidative stress is a key factor to aging, acetic acid resistance was associated with increased gene expression of TOR1, which encodes a key growth signaling kinase, and MSN2 and MSN4, which encode stress-responsive transcription factors. The addition of the antioxidant morin could counteract this increased expression, suggesting a possible modulatory role in cell signaling and the stress response of yeast. Therefore, yeast represents a versatile model organism and rapid screening tool to discover potentially rejuvenescent molecules with anti-aging and anti-oxidant potential from natural resources and to advance knowledge in the molecular study of stress and aging.https://www.frontiersin.org/articles/10.3389/fphar.2022.980066/fullflavonoidsquercetin analoguesmodel yeastoxidative stress responseanti-ageingmedicinal plant |
| spellingShingle | Hira Zahoor Kwanrutai Watchaputi Janejira Hata Wachirachai Pabuprapap Apichart Suksamrarn Lee Suan Chua Nitnipa Soontorngun Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plants Frontiers in Pharmacology flavonoids quercetin analogues model yeast oxidative stress response anti-ageing medicinal plant |
| title | Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plants |
| title_full | Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plants |
| title_fullStr | Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plants |
| title_full_unstemmed | Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plants |
| title_short | Model yeast as a versatile tool to examine the antioxidant and anti-ageing potential of flavonoids, extracted from medicinal plants |
| title_sort | model yeast as a versatile tool to examine the antioxidant and anti ageing potential of flavonoids extracted from medicinal plants |
| topic | flavonoids quercetin analogues model yeast oxidative stress response anti-ageing medicinal plant |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.980066/full |
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