Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection
The onion yellow dwarf virus (OYDV) represents a limiting biotic stress in onion (<i>Allium cepa</i> L.); little information is available regarding resistant varieties. In Italy, onion production is limited but represented by a wide diversity of ecotypes. A two-year trial was carried out...
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MDPI AG
2024-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/1/73 |
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| author | Carla Libia Corrado Giuseppe Micali Antonio Mauceri Sabrina Bertin Francesco Sunseri Maria Rosa Abenavoli Antonio Tiberini |
| author_facet | Carla Libia Corrado Giuseppe Micali Antonio Mauceri Sabrina Bertin Francesco Sunseri Maria Rosa Abenavoli Antonio Tiberini |
| author_sort | Carla Libia Corrado |
| collection | DOAJ |
| description | The onion yellow dwarf virus (OYDV) represents a limiting biotic stress in onion (<i>Allium cepa</i> L.); little information is available regarding resistant varieties. In Italy, onion production is limited but represented by a wide diversity of ecotypes. A two-year trial was carried out to test the OYDV-susceptibility/tolerance of different Italian onion cultivars by rating symptom severity and plant growth parameters and assessing post-harvest secondary infections. The cultivar and ecotypes included in the study were characterized by simple sequence repeats (SSR) analysis, and the expression analysis of two genes (Eukaryotic translation initiation factors, <i>EIFs</i>) involved in potyvirus replication was also performed. Two susceptible and one tolerant cultivar were identified based on symptom expression and virus impact on plants. Although differences in growth parameters were limited to the first-year trial, the infection was correlated to a higher incidence of secondary infections in post-harvest, with altered water balance in infected bulbs. This correlation was also demonstrated during the long-term storage of bulbs. SSR analysis identified different clusters and only one gene isoform (<i>EIF4e</i>iso1) showed different expression levels in the OYDV/onion pathosystem. In conclusion, this study defines the genetic profile of Italian onion cultivars and provides evidence on susceptibility/tolerance features which will be useful in the future for the identification of viral resistance traits in onion. |
| first_indexed | 2024-03-08T10:49:32Z |
| format | Article |
| id | doaj.art-9dfaf2a419304e65bb21ed64fa013f1d |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T10:49:32Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-9dfaf2a419304e65bb21ed64fa013f1d2024-01-26T16:49:53ZengMDPI AGHorticulturae2311-75242024-01-011017310.3390/horticulturae10010073Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) InfectionCarla Libia Corrado0Giuseppe Micali1Antonio Mauceri2Sabrina Bertin3Francesco Sunseri4Maria Rosa Abenavoli5Antonio Tiberini6Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro, 5, 00185 Rome, ItalyDepartment of Agraria, University Mediterranea of Reggio Calabria, Località Feo di Vito snc, 89124 Reggio Calabria, ItalyDepartment of Agraria, University Mediterranea of Reggio Calabria, Località Feo di Vito snc, 89124 Reggio Calabria, ItalyCouncil for Agricultural Research and Economics, Research Centre for Plant Protection and Certification, Via C. G. Bertero, 22, 00156 Rome, ItalyDepartment of Agraria, University Mediterranea of Reggio Calabria, Località Feo di Vito snc, 89124 Reggio Calabria, ItalyDepartment of Agraria, University Mediterranea of Reggio Calabria, Località Feo di Vito snc, 89124 Reggio Calabria, ItalyCouncil for Agricultural Research and Economics, Research Centre for Plant Protection and Certification, Via C. G. Bertero, 22, 00156 Rome, ItalyThe onion yellow dwarf virus (OYDV) represents a limiting biotic stress in onion (<i>Allium cepa</i> L.); little information is available regarding resistant varieties. In Italy, onion production is limited but represented by a wide diversity of ecotypes. A two-year trial was carried out to test the OYDV-susceptibility/tolerance of different Italian onion cultivars by rating symptom severity and plant growth parameters and assessing post-harvest secondary infections. The cultivar and ecotypes included in the study were characterized by simple sequence repeats (SSR) analysis, and the expression analysis of two genes (Eukaryotic translation initiation factors, <i>EIFs</i>) involved in potyvirus replication was also performed. Two susceptible and one tolerant cultivar were identified based on symptom expression and virus impact on plants. Although differences in growth parameters were limited to the first-year trial, the infection was correlated to a higher incidence of secondary infections in post-harvest, with altered water balance in infected bulbs. This correlation was also demonstrated during the long-term storage of bulbs. SSR analysis identified different clusters and only one gene isoform (<i>EIF4e</i>iso1) showed different expression levels in the OYDV/onion pathosystem. In conclusion, this study defines the genetic profile of Italian onion cultivars and provides evidence on susceptibility/tolerance features which will be useful in the future for the identification of viral resistance traits in onion.https://www.mdpi.com/2311-7524/10/1/73onion yellow dwarf virusonion cultivarsEIF genesSSR analysispost-harvest infections |
| spellingShingle | Carla Libia Corrado Giuseppe Micali Antonio Mauceri Sabrina Bertin Francesco Sunseri Maria Rosa Abenavoli Antonio Tiberini Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection Horticulturae onion yellow dwarf virus onion cultivars EIF genes SSR analysis post-harvest infections |
| title | Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection |
| title_full | Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection |
| title_fullStr | Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection |
| title_full_unstemmed | Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection |
| title_short | Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection |
| title_sort | study on italian onion cultivars ecotypes towards onion yellow dwarf virus oydv infection |
| topic | onion yellow dwarf virus onion cultivars EIF genes SSR analysis post-harvest infections |
| url | https://www.mdpi.com/2311-7524/10/1/73 |
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