Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis

Abstract In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon‐degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), r...

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Main Authors: Ivan Sazykin, Maksim Makarenko, Ludmila Khmelevtsova, Ekaterina Seliverstova, Alexander Rakin, Marina Sazykina
Format: Article
Language:English
Published: Wiley 2019-09-01
Series:MicrobiologyOpen
Subjects:
Online Access:https://doi.org/10.1002/mbo3.855
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author Ivan Sazykin
Maksim Makarenko
Ludmila Khmelevtsova
Ekaterina Seliverstova
Alexander Rakin
Marina Sazykina
author_facet Ivan Sazykin
Maksim Makarenko
Ludmila Khmelevtsova
Ekaterina Seliverstova
Alexander Rakin
Marina Sazykina
author_sort Ivan Sazykin
collection DOAJ
description Abstract In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon‐degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153, sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC. Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.
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spelling doaj.art-9dfc534031b94332a63262fe3dfd0c8b2022-12-21T18:40:10ZengWileyMicrobiologyOpen2045-88272019-09-0189n/an/a10.1002/mbo3.855Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolisIvan Sazykin0Maksim Makarenko1Ludmila Khmelevtsova2Ekaterina Seliverstova3Alexander Rakin4Marina Sazykina5Southern Federal University Rostov‐on‐Don Russian FederationSouthern Federal University Rostov‐on‐Don Russian FederationSouthern Federal University Rostov‐on‐Don Russian FederationSouthern Federal University Rostov‐on‐Don Russian FederationInstitute for Bacterial Infections and Zoonoses Friedrich‐Loeffler‐Institut, Federal Research Institute for Animal Health Jena GermanySouthern Federal University Rostov‐on‐Don Russian FederationAbstract In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon‐degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153, sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC. Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.https://doi.org/10.1002/mbo3.855cytochrome P450DNA repairhydrocarbonsROSsuperoxide dismutase
spellingShingle Ivan Sazykin
Maksim Makarenko
Ludmila Khmelevtsova
Ekaterina Seliverstova
Alexander Rakin
Marina Sazykina
Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
MicrobiologyOpen
cytochrome P450
DNA repair
hydrocarbons
ROS
superoxide dismutase
title Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
title_full Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
title_fullStr Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
title_full_unstemmed Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
title_short Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
title_sort cyclohexane naphthalene and diesel fuel increase oxidative stress cyp153 soda and reca gene expression in rhodococcus erythropolis
topic cytochrome P450
DNA repair
hydrocarbons
ROS
superoxide dismutase
url https://doi.org/10.1002/mbo3.855
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