Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm
Background: Exploring genetic diversity in traits of interest and utilizing it to breed high yielding, disease-resistant and high market value varieties is the prime objective of plant breeders. Unfortunately, genetic diversity in most of the crops has declined due to selection and reutilization of...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722006887 |
| _version_ | 1797952863031263232 |
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| author | Shahzadi Mahpara Lariab Fatima Ammara Gul Rehmat Ullah Muhammad Muneer Muhammad Ashraf Bhatti Javed Iqbal Affan Ahmed Tehseen Fatima Muhammad Usman Ijaz Sulaiman Ali Alharbi Wafa A. Alrasheed Muhammad Ihsan Ullah Abdul Rauf Muhammad Waqar Nasir Hesham Ali El Enshasy |
| author_facet | Shahzadi Mahpara Lariab Fatima Ammara Gul Rehmat Ullah Muhammad Muneer Muhammad Ashraf Bhatti Javed Iqbal Affan Ahmed Tehseen Fatima Muhammad Usman Ijaz Sulaiman Ali Alharbi Wafa A. Alrasheed Muhammad Ihsan Ullah Abdul Rauf Muhammad Waqar Nasir Hesham Ali El Enshasy |
| author_sort | Shahzadi Mahpara |
| collection | DOAJ |
| description | Background: Exploring genetic diversity in traits of interest and utilizing it to breed high yielding, disease-resistant and high market value varieties is the prime objective of plant breeders. Unfortunately, genetic diversity in most of the crops has declined due to selection and reutilization of already adapted germplasm. Chemical mutagens are widely used to create novel changes in germplasm; however, these have been rarely tested for chili germplasm in Pakistan. Methods: We used ethyl methane sulfonate (EMS) and sodium azide (SA) to induce mutations for obtaining novel alterations in morphological and yield-related traits of four chili genotypes (i.e., ‘Mexi’, ‘Dandi Cut’, ‘Nageena’ and ‘Talhari’). Firstly, concentrations of both mutagens were calibrated, i.e., 1.5 % EMS and 0.5 % SA. Afterwards, seeds of four chili cultivars were treated and grown in peat trays. As the nursery was established, seedlings were transferred to media (sand: silt: clay + 1 kg well-decomposed FYM) after 30 days of germination. Different mutants were isolated based on differential morphological and phenotypic expressions. Results: It was found that efficiency of EMS was greater than SA and more variants were noticed in case of EMS-treated populations. Genetic analysis showed that almost all the traits were affected by mutagens in the M1 generations although the stability of isolated mutants was not checked in later generations. All the traits showed higher estimates of heritability but in some traits genetic advance was non-significant. Conclusion: It is recommended that isolated mutants could be used as rich genetic diversity source to breed new varieties. Furthermore, EMS proved a suitable mutagen for targeted mutations. |
| first_indexed | 2024-04-10T22:52:47Z |
| format | Article |
| id | doaj.art-9e000a944c0d4a3ba95a3dc55876c893 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-10T22:52:47Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-9e000a944c0d4a3ba95a3dc55876c8932023-01-15T04:21:23ZengElsevierJournal of King Saud University: Science1018-36472023-02-01352102507Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasmShahzadi Mahpara0Lariab Fatima1Ammara Gul2Rehmat Ullah3Muhammad Muneer4Muhammad Ashraf Bhatti5Javed Iqbal6Affan Ahmed7Tehseen Fatima8Muhammad Usman Ijaz9Sulaiman Ali Alharbi10Wafa A. Alrasheed11Muhammad Ihsan Ullah12Abdul Rauf13Muhammad Waqar Nasir14Hesham Ali El Enshasy15Department of Plant Breeding & Genetics, Faculty of Agricultural Sciences and Technology, Ghazi University, Dera Ghazi Khan 32200, Pakistan; Corresponding author at: Department of Plant Breeding & Genetics, Ghazi University, Dera Ghazi Khan 32200, Pakistan.Civil Hospital, Sakhi Sarwar, Punjab, PakistanCivil Hospital, Fort Munro, Punjab, PakistanWheat Program, Crop Science Institute, National Agricultural Research Centre, Islamabad, PakistanSoil and Water Testing Laboratory, for Research, Dera Ghazi Khan, Punjab, PakistanSoil and Water Testing Laboratory Layyah, PakistanDepartment of Agricultural Engineering, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, PakistanDepartment of Plant Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences & Technology, Islamabad, PakistanDepartment of Plant Breeding & Genetics, Faculty of Agricultural Sciences and Technology, Ghazi University, Dera Ghazi Khan 32200, PakistanInstitute of Microbiology, University of Veterinary & Animal Sciences, Lahore, PakistanDepartment of Botany & Microbiology College of Science King Saud University, P.O Box 2455, Riyadh 11451, Saudi ArabiaNational Research and Development Center for Sustainable Agriculture (Estidamah), Riyadh Techno Valley, Riyadh 12373, Saudi ArabiaCotton Research Institute Multan, PakistanScientific Officer (Field) Dera Ghazi Khan, PakistanGeorgikon Campus Keszthely, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryUniversiti Teknologi Malaysia (UTM), Institute of Bioproduct Development (IBD), Skudai, Malaysia; Universiti Teknologi Malaysia (UTM), School of Chemical and Energy Engineering, Faculty of Engineering, Skudai, Malaysia; City of Scientific Research and Technology Applications (SRTA), Alexandria, EgyptBackground: Exploring genetic diversity in traits of interest and utilizing it to breed high yielding, disease-resistant and high market value varieties is the prime objective of plant breeders. Unfortunately, genetic diversity in most of the crops has declined due to selection and reutilization of already adapted germplasm. Chemical mutagens are widely used to create novel changes in germplasm; however, these have been rarely tested for chili germplasm in Pakistan. Methods: We used ethyl methane sulfonate (EMS) and sodium azide (SA) to induce mutations for obtaining novel alterations in morphological and yield-related traits of four chili genotypes (i.e., ‘Mexi’, ‘Dandi Cut’, ‘Nageena’ and ‘Talhari’). Firstly, concentrations of both mutagens were calibrated, i.e., 1.5 % EMS and 0.5 % SA. Afterwards, seeds of four chili cultivars were treated and grown in peat trays. As the nursery was established, seedlings were transferred to media (sand: silt: clay + 1 kg well-decomposed FYM) after 30 days of germination. Different mutants were isolated based on differential morphological and phenotypic expressions. Results: It was found that efficiency of EMS was greater than SA and more variants were noticed in case of EMS-treated populations. Genetic analysis showed that almost all the traits were affected by mutagens in the M1 generations although the stability of isolated mutants was not checked in later generations. All the traits showed higher estimates of heritability but in some traits genetic advance was non-significant. Conclusion: It is recommended that isolated mutants could be used as rich genetic diversity source to breed new varieties. Furthermore, EMS proved a suitable mutagen for targeted mutations.http://www.sciencedirect.com/science/article/pii/S1018364722006887CapsicumChemical mutagenCorrelationGenetic variabilityYield |
| spellingShingle | Shahzadi Mahpara Lariab Fatima Ammara Gul Rehmat Ullah Muhammad Muneer Muhammad Ashraf Bhatti Javed Iqbal Affan Ahmed Tehseen Fatima Muhammad Usman Ijaz Sulaiman Ali Alharbi Wafa A. Alrasheed Muhammad Ihsan Ullah Abdul Rauf Muhammad Waqar Nasir Hesham Ali El Enshasy Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm Journal of King Saud University: Science Capsicum Chemical mutagen Correlation Genetic variability Yield |
| title | Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm |
| title_full | Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm |
| title_fullStr | Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm |
| title_full_unstemmed | Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm |
| title_short | Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm |
| title_sort | exploring the efficiency of ethyl methane sulfonate ems and sodium azide sa to induce mutation in chili capsicum annuum l germplasm |
| topic | Capsicum Chemical mutagen Correlation Genetic variability Yield |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722006887 |
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