Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis.
Improved understanding of the immune events discriminating between seropositive arthralgia and clinical synovitis is of key importance in rheumatology research. Ample evidence suggests a role for Th17 cells in rheumatoid arthritis. We hypothesized that CD4+CD161+ cells representing Th17 lineage cell...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3815125?pdf=render |
| _version_ | 1818041155755966464 |
|---|---|
| author | Paulina Chalan Bart-Jan Kroesen Kornelis S M van der Geest Minke G Huitema Wayel H Abdulahad Johan Bijzet Elisabeth Brouwer Annemieke M H Boots |
| author_facet | Paulina Chalan Bart-Jan Kroesen Kornelis S M van der Geest Minke G Huitema Wayel H Abdulahad Johan Bijzet Elisabeth Brouwer Annemieke M H Boots |
| author_sort | Paulina Chalan |
| collection | DOAJ |
| description | Improved understanding of the immune events discriminating between seropositive arthralgia and clinical synovitis is of key importance in rheumatology research. Ample evidence suggests a role for Th17 cells in rheumatoid arthritis. We hypothesized that CD4+CD161+ cells representing Th17 lineage cells may be modulated prior to or after development of clinical synovitis. Therefore, in a cross-sectional study, we investigated the occurrence of CD4+CD161+ T-cells in seropositive arthralgia patients who are at risk for developing rheumatoid arthritis and in newly diagnosed rheumatoid arthritis patients. In a prospective study, we evaluated the effect of methotrexate treatment on circulating CD4+CD161+ T-cells. Next, we assessed if these cells can be detected at the level of the RA joints. Precursor Th17 lineage cells bearing CD161 were found to be increased in seropositive arthralgia patients. In contrast, circulating CD4+CD161+T-cells were decreased in newly diagnosed rheumatoid arthritis patients. The decrease in CD4+CD161+ T-cells correlated inversely with C-reactive protein and with the 66 swollen joint count. Methotrexate treatment led to normalization of CD4+CD161+ T-cells and reduced disease activity. CD4+CD161+ T cells were readily detected in synovial tissues from both early and late-stage rheumatoid arthritis. In addition, synovial fluid from late-stage disease was found to be enriched for CD4+CD161+ T-cells. Notably, synovial fluid accumulated CD4+CD161+T-cells showed skewing towards the Th1 phenotype as evidenced by increased interferon-γ expression. The changes in peripheral numbers of CD4+CD161+ T-cells in seropositive arthralgia and early rheumatoid arthritis and the enrichment of these cells at the level of the joint predict a role for CD4+CD161+ T-cells in the early immune events leading to clinical synovitis. Our findings may add to the development of RA prediction models and provide opportunities for early intervention. |
| first_indexed | 2024-12-10T08:25:56Z |
| format | Article |
| id | doaj.art-9e023a2bab484e2096a20549d3ca0911 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T08:25:56Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9e023a2bab484e2096a20549d3ca09112022-12-22T01:56:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7937010.1371/journal.pone.0079370Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis.Paulina ChalanBart-Jan KroesenKornelis S M van der GeestMinke G HuitemaWayel H AbdulahadJohan BijzetElisabeth BrouwerAnnemieke M H BootsImproved understanding of the immune events discriminating between seropositive arthralgia and clinical synovitis is of key importance in rheumatology research. Ample evidence suggests a role for Th17 cells in rheumatoid arthritis. We hypothesized that CD4+CD161+ cells representing Th17 lineage cells may be modulated prior to or after development of clinical synovitis. Therefore, in a cross-sectional study, we investigated the occurrence of CD4+CD161+ T-cells in seropositive arthralgia patients who are at risk for developing rheumatoid arthritis and in newly diagnosed rheumatoid arthritis patients. In a prospective study, we evaluated the effect of methotrexate treatment on circulating CD4+CD161+ T-cells. Next, we assessed if these cells can be detected at the level of the RA joints. Precursor Th17 lineage cells bearing CD161 were found to be increased in seropositive arthralgia patients. In contrast, circulating CD4+CD161+T-cells were decreased in newly diagnosed rheumatoid arthritis patients. The decrease in CD4+CD161+ T-cells correlated inversely with C-reactive protein and with the 66 swollen joint count. Methotrexate treatment led to normalization of CD4+CD161+ T-cells and reduced disease activity. CD4+CD161+ T cells were readily detected in synovial tissues from both early and late-stage rheumatoid arthritis. In addition, synovial fluid from late-stage disease was found to be enriched for CD4+CD161+ T-cells. Notably, synovial fluid accumulated CD4+CD161+T-cells showed skewing towards the Th1 phenotype as evidenced by increased interferon-γ expression. The changes in peripheral numbers of CD4+CD161+ T-cells in seropositive arthralgia and early rheumatoid arthritis and the enrichment of these cells at the level of the joint predict a role for CD4+CD161+ T-cells in the early immune events leading to clinical synovitis. Our findings may add to the development of RA prediction models and provide opportunities for early intervention.http://europepmc.org/articles/PMC3815125?pdf=render |
| spellingShingle | Paulina Chalan Bart-Jan Kroesen Kornelis S M van der Geest Minke G Huitema Wayel H Abdulahad Johan Bijzet Elisabeth Brouwer Annemieke M H Boots Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis. PLoS ONE |
| title | Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis. |
| title_full | Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis. |
| title_fullStr | Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis. |
| title_full_unstemmed | Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis. |
| title_short | Circulating CD4+CD161+ T lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis. |
| title_sort | circulating cd4 cd161 t lymphocytes are increased in seropositive arthralgia patients but decreased in patients with newly diagnosed rheumatoid arthritis |
| url | http://europepmc.org/articles/PMC3815125?pdf=render |
| work_keys_str_mv | AT paulinachalan circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis AT bartjankroesen circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis AT kornelissmvandergeest circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis AT minkeghuitema circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis AT wayelhabdulahad circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis AT johanbijzet circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis AT elisabethbrouwer circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis AT annemiekemhboots circulatingcd4cd161tlymphocytesareincreasedinseropositivearthralgiapatientsbutdecreasedinpatientswithnewlydiagnosedrheumatoidarthritis |