Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditions
Samples of C. ramosus and D. platypus were collected from Hurgada, the Red Sea during June-August 2018. Samples were transported to the lab at the University of Alexandria, Egypt. This study aims to describe the embryogenesis of C. ramosus inside the capsules and test the effects of signaling factor...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Egyptian Journal of Aquatic Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687428520300376 |
| _version_ | 1818408250866925568 |
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| author | Ibrahim Gaber Mauro Luisetto Oleg Latyshev |
| author_facet | Ibrahim Gaber Mauro Luisetto Oleg Latyshev |
| author_sort | Ibrahim Gaber |
| collection | DOAJ |
| description | Samples of C. ramosus and D. platypus were collected from Hurgada, the Red Sea during June-August 2018. Samples were transported to the lab at the University of Alexandria, Egypt. This study aims to describe the embryogenesis of C. ramosus inside the capsules and test the effects of signaling factors and neuro-transmitters on the in vitro transformation of larvae of D. platypus and identify some bioactive inducers that increase larval metamorphosis. Capsules of C. ramosus were fixed in 10% saline formaldehyde and capsules of D. platypus were placed in plastic aquaria for immune-histochemical test. The sequence of the embryonic transformations and the duration time of each step of C. ramosus were identified. The importance of the following experiment is to provide a suitable medium for the developing larval stages and improve eugenic manner of the new generations. Undertreated conditions of D. platypus larvae, MF 2.5 mM, C8:0 1 µM, ADMA 2.5 mM, and Acetylcholine 0.5 mM larval transformation from trochophore to veliger to early juvenile stages was accelerated than larvae of control in seawater. Serotonin 10 µg/ml and Acetylcholine 1 mM exerted a negative effect while C8:0 0.1 or 10 µM, MF 300 µM, ADMA 1 mM and 1.5 mM did not affect larval transformation. Atorvastatin (5, 20 and 40 mg), Pravastatin (50 mg and 100 mg), Cetirizine hydrochloride (10 mg), Nebivolol (2.5 mg, 5 mg. and 10 mg), Atenolol (25 mg and 100 mg) and Amlodipine Besylate (2.5 mg, 5.0 mg, and 10 mg) exerted a negative impact on larvae in all stages and mortality predominated. |
| first_indexed | 2024-12-14T09:40:45Z |
| format | Article |
| id | doaj.art-9e028521fc2f458dbe1c0fa4757c4440 |
| institution | Directory Open Access Journal |
| issn | 1687-4285 |
| language | English |
| last_indexed | 2024-12-14T09:40:45Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Egyptian Journal of Aquatic Research |
| spelling | doaj.art-9e028521fc2f458dbe1c0fa4757c44402022-12-21T23:07:47ZengElsevierEgyptian Journal of Aquatic Research1687-42852020-09-01463265271Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditionsIbrahim Gaber0Mauro Luisetto1Oleg Latyshev2Department of Zoology, Faculty of Science, Alexandria University, Egypt: Postal Code: 21547; Corresponding author.Applied Pharmacology, Turin and Pavia University 29121, IMA Academy, ItalyScience and Democracy Network Harvard University’s John F. Kennedy School of Government in Cambridge, USA. Postal Code: 02142; IMA academy, natural science branch RussiaSamples of C. ramosus and D. platypus were collected from Hurgada, the Red Sea during June-August 2018. Samples were transported to the lab at the University of Alexandria, Egypt. This study aims to describe the embryogenesis of C. ramosus inside the capsules and test the effects of signaling factors and neuro-transmitters on the in vitro transformation of larvae of D. platypus and identify some bioactive inducers that increase larval metamorphosis. Capsules of C. ramosus were fixed in 10% saline formaldehyde and capsules of D. platypus were placed in plastic aquaria for immune-histochemical test. The sequence of the embryonic transformations and the duration time of each step of C. ramosus were identified. The importance of the following experiment is to provide a suitable medium for the developing larval stages and improve eugenic manner of the new generations. Undertreated conditions of D. platypus larvae, MF 2.5 mM, C8:0 1 µM, ADMA 2.5 mM, and Acetylcholine 0.5 mM larval transformation from trochophore to veliger to early juvenile stages was accelerated than larvae of control in seawater. Serotonin 10 µg/ml and Acetylcholine 1 mM exerted a negative effect while C8:0 0.1 or 10 µM, MF 300 µM, ADMA 1 mM and 1.5 mM did not affect larval transformation. Atorvastatin (5, 20 and 40 mg), Pravastatin (50 mg and 100 mg), Cetirizine hydrochloride (10 mg), Nebivolol (2.5 mg, 5 mg. and 10 mg), Atenolol (25 mg and 100 mg) and Amlodipine Besylate (2.5 mg, 5.0 mg, and 10 mg) exerted a negative impact on larvae in all stages and mortality predominated.http://www.sciencedirect.com/science/article/pii/S1687428520300376C. ramosusD. platypusOvigerous capsuleEggCleavagePre-veliger |
| spellingShingle | Ibrahim Gaber Mauro Luisetto Oleg Latyshev Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditions Egyptian Journal of Aquatic Research C. ramosus D. platypus Ovigerous capsule Egg Cleavage Pre-veliger |
| title | Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditions |
| title_full | Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditions |
| title_fullStr | Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditions |
| title_full_unstemmed | Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditions |
| title_short | Intracapsular embryogenesis and larval development of Chicoreus ramosus (Linnaeus, 1758) and Dendropoma platypus (Mörch, 1861) (Gastropoda, Prosobranchia) under normal and treated conditions |
| title_sort | intracapsular embryogenesis and larval development of chicoreus ramosus linnaeus 1758 and dendropoma platypus morch 1861 gastropoda prosobranchia under normal and treated conditions |
| topic | C. ramosus D. platypus Ovigerous capsule Egg Cleavage Pre-veliger |
| url | http://www.sciencedirect.com/science/article/pii/S1687428520300376 |
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