MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma
The diagnosis of brain gliomas is mainly based on imaging methods. The gold standard in this area is MRI. Recommendations for the prevention, diagnosis, and treatment of gliomas are periodically modified and updated. One of the diagnostic techniques used when a brain glioma is suspected is liquid bi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/10/1477 |
| _version_ | 1827651324793061376 |
|---|---|
| author | Lukasz Oldak Sylwia Chludzinska-Kasperuk Patrycja Milewska Kamil Grubczak Joanna Reszec Ewa Gorodkiewicz |
| author_facet | Lukasz Oldak Sylwia Chludzinska-Kasperuk Patrycja Milewska Kamil Grubczak Joanna Reszec Ewa Gorodkiewicz |
| author_sort | Lukasz Oldak |
| collection | DOAJ |
| description | The diagnosis of brain gliomas is mainly based on imaging methods. The gold standard in this area is MRI. Recommendations for the prevention, diagnosis, and treatment of gliomas are periodically modified and updated. One of the diagnostic techniques used when a brain glioma is suspected is liquid biopsy. However, this technique requires further development to confirm its effectiveness. This paper presents a proposal of three potential biomarkers of brain gliomas—extracellular matrix metalloproteinase-1 (MMP-1), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), and the 20S proteasome—which were quantified in blood plasma using SPRi biosensors. A statistical analysis of the results indicated no significant changes in the concentrations between the control group (K) and grades G1 and G2, and similarly between grades G3 and G4. However, the differences in the concentrations between the groups K/G1/G2 and G3/G4 were statistically significant. A positive average correlation was found between the concentrations of the proteins and the patient’s age. The individual tested proteins were also highly correlated with each other. Our work proposes a new diagnostic technique that may aid in the diagnosis of brain gliomas. |
| first_indexed | 2024-03-09T20:37:09Z |
| format | Article |
| id | doaj.art-9e06800899b14c8f9531024c27465ed1 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T20:37:09Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9e06800899b14c8f9531024c27465ed12023-11-23T23:09:17ZengMDPI AGBiomolecules2218-273X2022-10-011210147710.3390/biom12101477MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain GliomaLukasz Oldak0Sylwia Chludzinska-Kasperuk1Patrycja Milewska2Kamil Grubczak3Joanna Reszec4Ewa Gorodkiewicz5Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, PolandBiobank, Biobank at Medical University of Bialystok, Waszyngtona 13, 15-269 Bialystok, PolandBiobank, Biobank at Medical University of Bialystok, Waszyngtona 13, 15-269 Bialystok, PolandDepartment of Regenerative Medicine and Immune Regulation, Medical Univeristy of Bialystok, Waszyngtona 13, 15-269 Bialystok, PolandBiobank, Biobank at Medical University of Bialystok, Waszyngtona 13, 15-269 Bialystok, PolandBioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, PolandThe diagnosis of brain gliomas is mainly based on imaging methods. The gold standard in this area is MRI. Recommendations for the prevention, diagnosis, and treatment of gliomas are periodically modified and updated. One of the diagnostic techniques used when a brain glioma is suspected is liquid biopsy. However, this technique requires further development to confirm its effectiveness. This paper presents a proposal of three potential biomarkers of brain gliomas—extracellular matrix metalloproteinase-1 (MMP-1), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), and the 20S proteasome—which were quantified in blood plasma using SPRi biosensors. A statistical analysis of the results indicated no significant changes in the concentrations between the control group (K) and grades G1 and G2, and similarly between grades G3 and G4. However, the differences in the concentrations between the groups K/G1/G2 and G3/G4 were statistically significant. A positive average correlation was found between the concentrations of the proteins and the patient’s age. The individual tested proteins were also highly correlated with each other. Our work proposes a new diagnostic technique that may aid in the diagnosis of brain gliomas.https://www.mdpi.com/2218-273X/12/10/1477gliomaliquid biopsySPRi biosensorbiomedical research |
| spellingShingle | Lukasz Oldak Sylwia Chludzinska-Kasperuk Patrycja Milewska Kamil Grubczak Joanna Reszec Ewa Gorodkiewicz MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma Biomolecules glioma liquid biopsy SPRi biosensor biomedical research |
| title | MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma |
| title_full | MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma |
| title_fullStr | MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma |
| title_full_unstemmed | MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma |
| title_short | MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma |
| title_sort | mmp 1 uch l1 and 20s proteasome as potential biomarkers supporting the diagnosis of brain glioma |
| topic | glioma liquid biopsy SPRi biosensor biomedical research |
| url | https://www.mdpi.com/2218-273X/12/10/1477 |
| work_keys_str_mv | AT lukaszoldak mmp1uchl1and20sproteasomeaspotentialbiomarkerssupportingthediagnosisofbrainglioma AT sylwiachludzinskakasperuk mmp1uchl1and20sproteasomeaspotentialbiomarkerssupportingthediagnosisofbrainglioma AT patrycjamilewska mmp1uchl1and20sproteasomeaspotentialbiomarkerssupportingthediagnosisofbrainglioma AT kamilgrubczak mmp1uchl1and20sproteasomeaspotentialbiomarkerssupportingthediagnosisofbrainglioma AT joannareszec mmp1uchl1and20sproteasomeaspotentialbiomarkerssupportingthediagnosisofbrainglioma AT ewagorodkiewicz mmp1uchl1and20sproteasomeaspotentialbiomarkerssupportingthediagnosisofbrainglioma |