Simultaneous readout of multiple FRET pairs using photochromism
Performing multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photochromism of the donor fluorophores.
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-22043-0 |
| _version_ | 1818432338095243264 |
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| author | Thijs Roebroek Wim Vandenberg François Sipieter Siewert Hugelier Christophe Stove Jin Zhang Peter Dedecker |
| author_facet | Thijs Roebroek Wim Vandenberg François Sipieter Siewert Hugelier Christophe Stove Jin Zhang Peter Dedecker |
| author_sort | Thijs Roebroek |
| collection | DOAJ |
| description | Performing multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photochromism of the donor fluorophores. |
| first_indexed | 2024-12-14T16:03:36Z |
| format | Article |
| id | doaj.art-9e086db852c1424faa90ec134becce7a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T16:03:36Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-9e086db852c1424faa90ec134becce7a2022-12-21T22:55:08ZengNature PortfolioNature Communications2041-17232021-03-0112111210.1038/s41467-021-22043-0Simultaneous readout of multiple FRET pairs using photochromismThijs Roebroek0Wim Vandenberg1François Sipieter2Siewert Hugelier3Christophe Stove4Jin Zhang5Peter Dedecker6Department of Chemistry, KU LeuvenDepartment of Chemistry, KU LeuvenCentre National de la Recherche Scientifique, Institut Jacques Monod, Université de ParisDepartment of Chemistry, KU LeuvenDepartment of Bioanalysis, Ghent UniversityDepartment of Pharmacology, University of California at San DiegoDepartment of Chemistry, KU LeuvenPerforming multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photochromism of the donor fluorophores.https://doi.org/10.1038/s41467-021-22043-0 |
| spellingShingle | Thijs Roebroek Wim Vandenberg François Sipieter Siewert Hugelier Christophe Stove Jin Zhang Peter Dedecker Simultaneous readout of multiple FRET pairs using photochromism Nature Communications |
| title | Simultaneous readout of multiple FRET pairs using photochromism |
| title_full | Simultaneous readout of multiple FRET pairs using photochromism |
| title_fullStr | Simultaneous readout of multiple FRET pairs using photochromism |
| title_full_unstemmed | Simultaneous readout of multiple FRET pairs using photochromism |
| title_short | Simultaneous readout of multiple FRET pairs using photochromism |
| title_sort | simultaneous readout of multiple fret pairs using photochromism |
| url | https://doi.org/10.1038/s41467-021-22043-0 |
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