The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes
Purpose: The purpose of the current study was to evaluate the role of - tocopherol in inhibiting ovarian oxidative stress, changes in ovarian follicles, estradiol levels, and the thickness of the endometrium in rats exposed to tobacco smoke. Material and Methods: Forty female rats were...
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| Format: | Article |
| Language: | English |
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Cukurova University
2014-04-01
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| Series: | Çukurova Üniversitesi Tıp Fakültesi Dergisi |
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| Online Access: | http://www.scopemed.org/fulltextpdf.php?mno=149417 |
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| author | Rahajeng Siti Nur Ratnawati Ika Nur Saputri Retti Ratnawati Setyawati Soeharto I Wayan Arsana Wiyasa |
| author_facet | Rahajeng Siti Nur Ratnawati Ika Nur Saputri Retti Ratnawati Setyawati Soeharto I Wayan Arsana Wiyasa |
| author_sort | Rahajeng Siti Nur Ratnawati |
| collection | DOAJ |
| description | Purpose: The purpose of the current study was to evaluate the role of - tocopherol in inhibiting ovarian oxidative stress, changes in ovarian follicles, estradiol levels, and the thickness of the endometrium in rats exposed to tobacco smoke.
Material and Methods: Forty female rats were divided into five groups consisting of a control group; group exposed to tobacco smoke; groups exposed to tobacco smokes receiving  - tocopherol supplementation at doses of 100; 200, and 400 mg/kg. Exposure to tobacco smoke was induced using smoke pumping equipment which was designed and made available in the Pharmacology Laboratory, Faculty of Medicine, Brawijaya University. Analysis of MDA level was done colorimetrically. Analysis of the number of ovarian follicles and endometrial thickness was done histopathologically with hematoxylin eosin staining. Analysis of estradiol level was done by ELISA technique.
Results: Exposure to tobacco smoke in rats can increase ovarian MDA level (0.388 ± 0.085) significantly compared to the control (0.121 ± 0.026) (P> 0.05). -tocopherol in various doses can reduce MDA level significantly compared to the group exposed to tobacco smoke (P <0.05), but has not reached the levels of the control group. Exposure to tobacco smoke can reduce the number of primary and secondary follicles significantly compared to the control (P <0.05), and tended to decrease the number of De Graff follicles in the ovaries despite not significant (P> 0.05). Administration of -tocopherol in different doses increased the number of secondary follicles which reached the number in the control group (P> 0.05). The number of primary follicles in the -tocopherol group of the first dose (3.8 ± 1.095) was comparable to the control group (3.8 ± 1.095) (P> 0.05). The numbers of secondary follicles in the -tocopherol group of the first dose (5.2 ± 1.304) and of the second dose (5.4 ± 0.548) were significantly higher than the control group (3.6 ± 0.548) (P < 0.05). Tobacco smoke lowered estradiol level (10.815 ± 2.374) significantly compared with no exposure (21.354 ± 4.215) (P < 0.05). Administration of -tocopherol in different doses increased estradiol level, reaching the level in the control group (P > 0.05). Tobacco smoke lowered thickness of endometrium (209.491 ± 38.635) significantly compared with no exposure (600.265 ± 76.563) (P < 0.05). The -tocopherol of the first dose (604. 569 ± 33.621) and of the second dose (609.459 ± 37.105) increase estradiol levels, reaching the level in the control group (600.265 ± 76.563) (P > 0.05).
Conclusion: Regarding the results describe above, it was concluded that -tocopherol could prevent oxidative damage and loss of ovarian function due to exposure to tobacco smoke characterized by decreased levels of MDA, increases in primary and secondary follicles, as well as the hormone estradiol. Furthermore, it also increased the thickness of endometrium. [Cukurova Med J 2014; 39(2.000): 203-212] |
| first_indexed | 2024-04-10T12:40:47Z |
| format | Article |
| id | doaj.art-9e087a9889b84e569b07a92bbdb7d734 |
| institution | Directory Open Access Journal |
| issn | 0250-5150 |
| language | English |
| last_indexed | 2024-04-10T12:40:47Z |
| publishDate | 2014-04-01 |
| publisher | Cukurova University |
| record_format | Article |
| series | Çukurova Üniversitesi Tıp Fakültesi Dergisi |
| spelling | doaj.art-9e087a9889b84e569b07a92bbdb7d7342023-02-15T16:14:17ZengCukurova UniversityÇukurova Üniversitesi Tıp Fakültesi Dergisi0250-51502014-04-01392203212149417The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco SmokesRahajeng Siti Nur Ratnawati0Ika Nur Saputri1Retti Ratnawati2Setyawati Soeharto3I Wayan Arsana Wiyasa4Midwifery Master Study Prgramme, Brawijaya University, Malang, East Java, Indonesia Midwifery Master Study Prgramme, Brawijaya University, Malang, East Java, Indonesia Laboratory of Physiology, Faculty of Medicine University of Brawijaya, East Java, Indonesia Laboratory of Pharmacology, Faculty of Medicine, University of Brawijaya, East Java, Indonesia Division of Fertility, Endocrinology and Reproduction, Obstetric and Ginaecology Laboratory, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, IndonesiaPurpose: The purpose of the current study was to evaluate the role of - tocopherol in inhibiting ovarian oxidative stress, changes in ovarian follicles, estradiol levels, and the thickness of the endometrium in rats exposed to tobacco smoke. Material and Methods: Forty female rats were divided into five groups consisting of a control group; group exposed to tobacco smoke; groups exposed to tobacco smokes receiving  - tocopherol supplementation at doses of 100; 200, and 400 mg/kg. Exposure to tobacco smoke was induced using smoke pumping equipment which was designed and made available in the Pharmacology Laboratory, Faculty of Medicine, Brawijaya University. Analysis of MDA level was done colorimetrically. Analysis of the number of ovarian follicles and endometrial thickness was done histopathologically with hematoxylin eosin staining. Analysis of estradiol level was done by ELISA technique. Results: Exposure to tobacco smoke in rats can increase ovarian MDA level (0.388 ± 0.085) significantly compared to the control (0.121 ± 0.026) (P> 0.05). -tocopherol in various doses can reduce MDA level significantly compared to the group exposed to tobacco smoke (P <0.05), but has not reached the levels of the control group. Exposure to tobacco smoke can reduce the number of primary and secondary follicles significantly compared to the control (P <0.05), and tended to decrease the number of De Graff follicles in the ovaries despite not significant (P> 0.05). Administration of -tocopherol in different doses increased the number of secondary follicles which reached the number in the control group (P> 0.05). The number of primary follicles in the -tocopherol group of the first dose (3.8 ± 1.095) was comparable to the control group (3.8 ± 1.095) (P> 0.05). The numbers of secondary follicles in the -tocopherol group of the first dose (5.2 ± 1.304) and of the second dose (5.4 ± 0.548) were significantly higher than the control group (3.6 ± 0.548) (P < 0.05). Tobacco smoke lowered estradiol level (10.815 ± 2.374) significantly compared with no exposure (21.354 ± 4.215) (P < 0.05). Administration of -tocopherol in different doses increased estradiol level, reaching the level in the control group (P > 0.05). Tobacco smoke lowered thickness of endometrium (209.491 ± 38.635) significantly compared with no exposure (600.265 ± 76.563) (P < 0.05). The -tocopherol of the first dose (604. 569 ± 33.621) and of the second dose (609.459 ± 37.105) increase estradiol levels, reaching the level in the control group (600.265 ± 76.563) (P > 0.05). Conclusion: Regarding the results describe above, it was concluded that -tocopherol could prevent oxidative damage and loss of ovarian function due to exposure to tobacco smoke characterized by decreased levels of MDA, increases in primary and secondary follicles, as well as the hormone estradiol. Furthermore, it also increased the thickness of endometrium. [Cukurova Med J 2014; 39(2.000): 203-212]http://www.scopemed.org/fulltextpdf.php?mno=149417oxidative stress; ovaries; endometrium; estrogen; antioxidant |
| spellingShingle | Rahajeng Siti Nur Ratnawati Ika Nur Saputri Retti Ratnawati Setyawati Soeharto I Wayan Arsana Wiyasa The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes Çukurova Üniversitesi Tıp Fakültesi Dergisi oxidative stress; ovaries; endometrium; estrogen; antioxidant |
| title | The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes |
| title_full | The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes |
| title_fullStr | The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes |
| title_full_unstemmed | The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes |
| title_short | The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes |
| title_sort | effect of alpha tocopherol on oxidative stress and ovarian function in rats exposed to tobacco smokes |
| topic | oxidative stress; ovaries; endometrium; estrogen; antioxidant |
| url | http://www.scopemed.org/fulltextpdf.php?mno=149417 |
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