Essential oil mixture on rumen fermentation and microbial community – an study
Objective The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2019-06-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-18-0652.pdf |
| _version_ | 1819000007842332672 |
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| author | Hanbeen Kim Eunsang Jung Hyo Gun Lee Byeongwoo Kim Seongkeun Cho Seyoung Lee Inhyuk Kwon Jakyeom Seo |
| author_facet | Hanbeen Kim Eunsang Jung Hyo Gun Lee Byeongwoo Kim Seongkeun Cho Seyoung Lee Inhyuk Kwon Jakyeom Seo |
| author_sort | Hanbeen Kim |
| collection | DOAJ |
| description | Objective The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g EOM/kg of substrate), and EOM 0.2 (supplementation of 2 g EOM/kg of substrate). An in vitro fermentation experiment was carried out using strained rumen fluid for 12 and 24 h incubation periods. At each time point, in vitro dry matter digestibility (IVDMD), neutral detergent fiber digestibility (IVNDFD), pH, ammonia nitrogen (NH3-N), and volatile fatty acid (VFA) concentrations, and relative microbial diversity were estimated. Results After 24 h incubation, treatments involving EOM supplementation led to significantly higher IVDMD (treatments and quadratic effect; p = 0.019 and 0.008) and IVNDFD (linear effect; p = 0.068) than did the CON treatment. The EOM 0.2 supplementation group had the highest NH3-N concentration (treatments; p = 0.032). Both EOM supplementations did not affect total VFA concentration and the proportion of individual VFAs; however, total VFA tended to increase in EOM supplementation groups, after 12 h incubation (linear; p = 0.071). Relative protozoa abundance significantly increased following EOM supplementation (treatments, p<0.001). Selenomonas ruminantium and Ruminococcus albus (treatments; p<0.001 and p = 0.005), abundance was higher in the EOM 0.1 treatment group than in CON. The abundance of Butyrivibrio fibrisolvens, fungi and Ruminococcus flavefaciens (treatments; p< 0.001, p<0.001, and p = 0.005) was higher following EOM 0.2 treatment. Conclusion The addition of newly developed EOM increased IVDMD, IVNDFD, and tended to increase total VFA indicating that it may be used as a feed additive to improve rumen fermentation by modulating rumen microbial communities. Further studies would be required to investigate the detailed metabolic mechanism underlying the effects of EOM supplementation. |
| first_indexed | 2024-12-20T22:26:29Z |
| format | Article |
| id | doaj.art-9e09f53f69394b2587d570b589a04c84 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-20T22:26:29Z |
| publishDate | 2019-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-9e09f53f69394b2587d570b589a04c842022-12-21T19:24:48ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172019-06-0132680881410.5713/ajas.18.065224137Essential oil mixture on rumen fermentation and microbial community – an studyHanbeen Kim0Eunsang Jung1Hyo Gun Lee2Byeongwoo Kim3Seongkeun Cho4Seyoung Lee5Inhyuk Kwon6Jakyeom Seo7 Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea Department of Bioenvironmental Energy, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea Division of Animal Husbandry, Yonam College, Cheonan 31005, Korea EASY BIO, Inc., Seoul 06253, Korea Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaObjective The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g EOM/kg of substrate), and EOM 0.2 (supplementation of 2 g EOM/kg of substrate). An in vitro fermentation experiment was carried out using strained rumen fluid for 12 and 24 h incubation periods. At each time point, in vitro dry matter digestibility (IVDMD), neutral detergent fiber digestibility (IVNDFD), pH, ammonia nitrogen (NH3-N), and volatile fatty acid (VFA) concentrations, and relative microbial diversity were estimated. Results After 24 h incubation, treatments involving EOM supplementation led to significantly higher IVDMD (treatments and quadratic effect; p = 0.019 and 0.008) and IVNDFD (linear effect; p = 0.068) than did the CON treatment. The EOM 0.2 supplementation group had the highest NH3-N concentration (treatments; p = 0.032). Both EOM supplementations did not affect total VFA concentration and the proportion of individual VFAs; however, total VFA tended to increase in EOM supplementation groups, after 12 h incubation (linear; p = 0.071). Relative protozoa abundance significantly increased following EOM supplementation (treatments, p<0.001). Selenomonas ruminantium and Ruminococcus albus (treatments; p<0.001 and p = 0.005), abundance was higher in the EOM 0.1 treatment group than in CON. The abundance of Butyrivibrio fibrisolvens, fungi and Ruminococcus flavefaciens (treatments; p< 0.001, p<0.001, and p = 0.005) was higher following EOM 0.2 treatment. Conclusion The addition of newly developed EOM increased IVDMD, IVNDFD, and tended to increase total VFA indicating that it may be used as a feed additive to improve rumen fermentation by modulating rumen microbial communities. Further studies would be required to investigate the detailed metabolic mechanism underlying the effects of EOM supplementation.http://www.ajas.info/upload/pdf/ajas-18-0652.pdfFeed Additive DegradabilityMicrobial Abundance |
| spellingShingle | Hanbeen Kim Eunsang Jung Hyo Gun Lee Byeongwoo Kim Seongkeun Cho Seyoung Lee Inhyuk Kwon Jakyeom Seo Essential oil mixture on rumen fermentation and microbial community – an study Asian-Australasian Journal of Animal Sciences Feed Additive Degradability Microbial Abundance |
| title | Essential oil mixture on rumen fermentation and microbial community – an study |
| title_full | Essential oil mixture on rumen fermentation and microbial community – an study |
| title_fullStr | Essential oil mixture on rumen fermentation and microbial community – an study |
| title_full_unstemmed | Essential oil mixture on rumen fermentation and microbial community – an study |
| title_short | Essential oil mixture on rumen fermentation and microbial community – an study |
| title_sort | essential oil mixture on rumen fermentation and microbial community an study |
| topic | Feed Additive Degradability Microbial Abundance |
| url | http://www.ajas.info/upload/pdf/ajas-18-0652.pdf |
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