A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen)
Bean common mosaic virus from the genus <i>Potyvirus</i> has a wide range of hosts and a very negative impact on cultivated crops from the genus <i>Phaseolus</i>. The risk of viral infection of economically important crops increases even if the carriers of the virus are relat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/7/1677 |
| _version_ | 1797590663711162368 |
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| author | Daniel Mihálik Simona Grešíková Richard Hančinský Pavel Cejnar Michaela Havrlentová Ján Kraic |
| author_facet | Daniel Mihálik Simona Grešíková Richard Hančinský Pavel Cejnar Michaela Havrlentová Ján Kraic |
| author_sort | Daniel Mihálik |
| collection | DOAJ |
| description | Bean common mosaic virus from the genus <i>Potyvirus</i> has a wide range of hosts and a very negative impact on cultivated crops from the genus <i>Phaseolus</i>. The risk of viral infection of economically important crops increases even if the carriers of the virus are related plant species growing on agroecological interfaces. Such plant species have emerged as new hosts for BCMV, usually harboring novel genetic variants of the virus. A novel genetic variant of BCMV was isolated from a symptomatic crownvetch plant, where the presence of this virus was confirmed via Western blot analysis and via amino acid identities in peptide fragments of CI, HC-pro, and CP proteins using the nanoLC-ESI-Q-TOF. The novel BCMV SVK isolate differed from the most genetically similar one in 0.91% of nucleotides and 1.55% of amino acids. The highest number of amino acid substitutions (8.8% of amino acids) was in the P1 protein, followed by CP (2.44% of amino acids). Minor substitutions were in Hc-pro, CI, and Nib proteins. The symptomatic crownvetch plant was confirmed as a new host and carrier of the novel BCMV isolate. |
| first_indexed | 2024-03-11T01:23:44Z |
| format | Article |
| id | doaj.art-9e0d3faab52443bdb7e53e2855143a66 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T01:23:44Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-9e0d3faab52443bdb7e53e2855143a662023-11-18T17:54:31ZengMDPI AGAgronomy2073-43952023-06-01137167710.3390/agronomy13071677A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen)Daniel Mihálik0Simona Grešíková1Richard Hančinský2Pavel Cejnar3Michaela Havrlentová4Ján Kraic5Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Nám. J. Herdu 2, 917 01 Trnava, SlovakiaDepartment of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Nám. J. Herdu 2, 917 01 Trnava, SlovakiaDepartment of Applied Biology and Genetics, Research Institute of Plant Production, National Agricultural and Food Centre, Bratislavská Cesta 122, 921 68 Piešťany, SlovakiaDepartment of Mathematics, Informatics and Cybernetics, Faculty of Chemical Engineering, University of Chemistry and Technology, Technická 5, Dejvice, 166 28 Prague, Czech RepublicDepartment of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Nám. J. Herdu 2, 917 01 Trnava, SlovakiaDepartment of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Nám. J. Herdu 2, 917 01 Trnava, SlovakiaBean common mosaic virus from the genus <i>Potyvirus</i> has a wide range of hosts and a very negative impact on cultivated crops from the genus <i>Phaseolus</i>. The risk of viral infection of economically important crops increases even if the carriers of the virus are related plant species growing on agroecological interfaces. Such plant species have emerged as new hosts for BCMV, usually harboring novel genetic variants of the virus. A novel genetic variant of BCMV was isolated from a symptomatic crownvetch plant, where the presence of this virus was confirmed via Western blot analysis and via amino acid identities in peptide fragments of CI, HC-pro, and CP proteins using the nanoLC-ESI-Q-TOF. The novel BCMV SVK isolate differed from the most genetically similar one in 0.91% of nucleotides and 1.55% of amino acids. The highest number of amino acid substitutions (8.8% of amino acids) was in the P1 protein, followed by CP (2.44% of amino acids). Minor substitutions were in Hc-pro, CI, and Nib proteins. The symptomatic crownvetch plant was confirmed as a new host and carrier of the novel BCMV isolate.https://www.mdpi.com/2073-4395/13/7/1677BCMVgenomeamino acid substitutionsnovel host |
| spellingShingle | Daniel Mihálik Simona Grešíková Richard Hančinský Pavel Cejnar Michaela Havrlentová Ján Kraic A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen) Agronomy BCMV genome amino acid substitutions novel host |
| title | A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen) |
| title_full | A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen) |
| title_fullStr | A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen) |
| title_full_unstemmed | A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen) |
| title_short | A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (<i>Securigera varia</i> L. Lassen) |
| title_sort | novel isolate of bean common mosaic virus isolated from crownvetch i securigera varia i l lassen |
| topic | BCMV genome amino acid substitutions novel host |
| url | https://www.mdpi.com/2073-4395/13/7/1677 |
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