Effects of Over-Expression of LOC92912 Gene on Cell Cycle Progression
Background: We had previously identified the genes involvedin squamous cell carcinoma of the head and neck using differentialdisplay and DNA microarray techniques. We also reportedthe first analytical study on a novel human gene calledLOC92912, which was identified by differential display as agene u...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2009-12-01
|
| Series: | Iranian Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://ijms.sums.ac.ir/files/PDFfiles/08-Dr_%20Seghatoleslam.pdf |
| Summary: | Background: We had previously identified the genes involvedin squamous cell carcinoma of the head and neck using differentialdisplay and DNA microarray techniques. We also reportedthe first analytical study on a novel human gene calledLOC92912, which was identified by differential display as agene up-regulated in such carcinomas. LOC92912, which is aputative member of the E2 ubiquitin conjugating enzyme family,is located on chromosome 15q and encodes a protein of 375amino acids. In this study, we present the extended analysis ofLOC92912 gene in order to uncover the pathway implicated incancer development or progression. We established a series ofRPMI 2650 cell line permanently over-expressing LOC92912gene, together with their related controls.Methods: LOC92912 gene was cloned in pSG5-expressingvector. In vitro translation assay was performed using pSG5-expressing LOC92912. The construct was used for transientand permanent transfection of LOC92912 gene into RPMI2650 cell line. Cell cycle analysis, clonogenicity, and cellgrowth assay for cells permanently over-expressing LOC92912were performed. Focus-like formation studies, also, wereinvestigated on cells permanently over-expressing LOC92912.Results: We found that RPMI 2650 cells permanently overexpressingLOC92912 show an increase in the number of cellsaccumulated in G0/G1 phase of the cell cycle, a decrease inclonogenicity and cell growth and formation of focus-likestructures. Preliminary data also showed changes in cell shapeand cell-cell adhesion.Conclusion: Our results demonstrated that LOC92912 inducedalterations in the proliferation of cells and might represent aputative novel regulator of cell cycle and some other cellularfunctions. This novel human gene may also represent a newtarget for treatment of cancers. |
|---|---|
| ISSN: | 0253-0716 1735-3688 |