Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the poly...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-01-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201501-0008_bilateral-hydronephrosis-and-hydroureter-after-ovariohysterectomy-using-nylon-cable-tie-a-case-report.php |
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| author | L.R. Mesquita S.C. Rahal L.M. Matsubara M.J. Mamprim C.R. Foschini L.G. Faria W.T. Kano |
| author_facet | L.R. Mesquita S.C. Rahal L.M. Matsubara M.J. Mamprim C.R. Foschini L.G. Faria W.T. Kano |
| author_sort | L.R. Mesquita |
| collection | DOAJ |
| description | Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first tested with positive and negative control DNA and its sensitivity and specificity were determined. A new polymerase chain reaction protocol was developed by combining the new primer pair sets with bovine (12SM-FW and 12SBT-REV2) and ruminant host-specific primer sets (12S-FW and 12S-REV). In conclusion, the developed polymerase chain reaction assays can potentially be used for direct detection of Chlamydophila abortus in bovine and ruminant samples. |
| first_indexed | 2024-04-10T07:59:54Z |
| format | Article |
| id | doaj.art-9e128c7c928344c8914f356f5585e4f3 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:54Z |
| publishDate | 2015-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-9e128c7c928344c8914f356f5585e4f32023-02-23T03:50:23ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922015-01-01601525610.17221/7926-VETMEDvet-201501-0008Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case reportL.R. Mesquita0S.C. Rahal1L.M. Matsubara2M.J. Mamprim3C.R. Foschini4L.G. Faria5W.T. Kano6School of Veterinary Medicine and Animal Science, University Estadual Paulista (UNESP), Botucatu, Sao Paulo, BrazilSchool of Veterinary Medicine and Animal Science, University Estadual Paulista (UNESP), Botucatu, Sao Paulo, BrazilSchool of Veterinary Medicine and Animal Science, University Estadual Paulista (UNESP), Botucatu, Sao Paulo, BrazilSchool of Veterinary Medicine and Animal Science, University Estadual Paulista (UNESP), Botucatu, Sao Paulo, BrazilSchool of Engineer of Bauru (Foschini), University Estadual Paulista (UNESP), Bauru, Sao Paulo, BrazilSchool of Agricultural and Veterinary Sciences, University Estadual Paulista (UNESP), Jaboticabal, Sao Paulo, BrazilSchool of Veterinary Medicine and Animal Science, University Estadual Paulista (UNESP), Botucatu, Sao Paulo, BrazilChlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first tested with positive and negative control DNA and its sensitivity and specificity were determined. A new polymerase chain reaction protocol was developed by combining the new primer pair sets with bovine (12SM-FW and 12SBT-REV2) and ruminant host-specific primer sets (12S-FW and 12S-REV). In conclusion, the developed polymerase chain reaction assays can potentially be used for direct detection of Chlamydophila abortus in bovine and ruminant samples.https://vetmed.agriculturejournals.cz/artkey/vet-201501-0008_bilateral-hydronephrosis-and-hydroureter-after-ovariohysterectomy-using-nylon-cable-tie-a-case-report.phpchlamydophila abortusinternal controlpcr |
| spellingShingle | L.R. Mesquita S.C. Rahal L.M. Matsubara M.J. Mamprim C.R. Foschini L.G. Faria W.T. Kano Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report Veterinární Medicína chlamydophila abortus internal control pcr |
| title | Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report |
| title_full | Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report |
| title_fullStr | Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report |
| title_full_unstemmed | Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report |
| title_short | Bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie: a case report |
| title_sort | bilateral hydronephrosis and hydroureter after ovariohysterectomy using nylon cable tie a case report |
| topic | chlamydophila abortus internal control pcr |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201501-0008_bilateral-hydronephrosis-and-hydroureter-after-ovariohysterectomy-using-nylon-cable-tie-a-case-report.php |
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