Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified Product
Dialyzable leukocyte extracts are clinically used under different commercial names (IMMUNEPOTENT CRP<sup>®</sup>, IMMODIN<sup>®</sup>, Transferon<sup>®</sup>) to modulate the immune response altered by pathological conditions such as cancer, inflammation, and vira...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/8/3505 |
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| author | Moisés Armides Franco-Molina Silvia Elena Santana-Krímskaya Diana Ginette Zarate-Triviño Pablo Zapata-Benavides Sara Paola Hernández-Martínez Fernanda Cervantes-Wong Cristina Rodríguez-Padilla |
| author_facet | Moisés Armides Franco-Molina Silvia Elena Santana-Krímskaya Diana Ginette Zarate-Triviño Pablo Zapata-Benavides Sara Paola Hernández-Martínez Fernanda Cervantes-Wong Cristina Rodríguez-Padilla |
| author_sort | Moisés Armides Franco-Molina |
| collection | DOAJ |
| description | Dialyzable leukocyte extracts are clinically used under different commercial names (IMMUNEPOTENT CRP<sup>®</sup>, IMMODIN<sup>®</sup>, Transferon<sup>®</sup>) to modulate the immune response altered by pathological conditions such as cancer, inflammation, and viral infections. The purpose of this study is to improve the production process of bovine dialyzable leukocyte extract without decreasing its biological activities (antioxidant, anti-inflammatory, and antitumoral). Our product modification consists of adding a dry heating step in the production process. In this study, we evaluated and compared the chemical composition (bromatological analysis), physical structure (infrared spectroscopy, X-ray diffraction, SEM, and zeta potential) and biological function of the dialyzable leukocyte extracts obtained from fresh and dry bovine spleens. Our results showed that the use of a drying step in the production process of the bovine dialyzable leukocyte extract (bDLE) did not affect its antioxidant and anti-inflammatory effects and it improved its antitumor properties. We suggest that this process modification could be applied to other biological products, such as dialyzable leukocyte extracts derived from other sources, in order to improve its functionality and formulation. |
| first_indexed | 2024-03-10T12:20:28Z |
| format | Article |
| id | doaj.art-9e14012b752d4b4cb6c3d0240f3c9650 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T12:20:28Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-9e14012b752d4b4cb6c3d0240f3c96502023-11-21T15:31:22ZengMDPI AGApplied Sciences2076-34172021-04-01118350510.3390/app11083505Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified ProductMoisés Armides Franco-Molina0Silvia Elena Santana-Krímskaya1Diana Ginette Zarate-Triviño2Pablo Zapata-Benavides3Sara Paola Hernández-Martínez4Fernanda Cervantes-Wong5Cristina Rodríguez-Padilla6Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, San Nicolás de los Garza 66455, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, San Nicolás de los Garza 66455, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, San Nicolás de los Garza 66455, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, San Nicolás de los Garza 66455, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, San Nicolás de los Garza 66455, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, San Nicolás de los Garza 66455, MexicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 “F”, San Nicolás de los Garza 66455, MexicoDialyzable leukocyte extracts are clinically used under different commercial names (IMMUNEPOTENT CRP<sup>®</sup>, IMMODIN<sup>®</sup>, Transferon<sup>®</sup>) to modulate the immune response altered by pathological conditions such as cancer, inflammation, and viral infections. The purpose of this study is to improve the production process of bovine dialyzable leukocyte extract without decreasing its biological activities (antioxidant, anti-inflammatory, and antitumoral). Our product modification consists of adding a dry heating step in the production process. In this study, we evaluated and compared the chemical composition (bromatological analysis), physical structure (infrared spectroscopy, X-ray diffraction, SEM, and zeta potential) and biological function of the dialyzable leukocyte extracts obtained from fresh and dry bovine spleens. Our results showed that the use of a drying step in the production process of the bovine dialyzable leukocyte extract (bDLE) did not affect its antioxidant and anti-inflammatory effects and it improved its antitumor properties. We suggest that this process modification could be applied to other biological products, such as dialyzable leukocyte extracts derived from other sources, in order to improve its functionality and formulation.https://www.mdpi.com/2076-3417/11/8/3505dialyzable leukocyte extractcharacterizationimmunotherapyantioxidantanti-inflammatoryantitumor |
| spellingShingle | Moisés Armides Franco-Molina Silvia Elena Santana-Krímskaya Diana Ginette Zarate-Triviño Pablo Zapata-Benavides Sara Paola Hernández-Martínez Fernanda Cervantes-Wong Cristina Rodríguez-Padilla Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified Product Applied Sciences dialyzable leukocyte extract characterization immunotherapy antioxidant anti-inflammatory antitumor |
| title | Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified Product |
| title_full | Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified Product |
| title_fullStr | Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified Product |
| title_full_unstemmed | Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified Product |
| title_short | Bovine Dialyzable Leukocyte Extract IMMUNEPOTENT CRP: Evaluation of Biological Activity of the Modified Product |
| title_sort | bovine dialyzable leukocyte extract immunepotent crp evaluation of biological activity of the modified product |
| topic | dialyzable leukocyte extract characterization immunotherapy antioxidant anti-inflammatory antitumor |
| url | https://www.mdpi.com/2076-3417/11/8/3505 |
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