Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food
Background & Objective: Lactic acid bacteria (LAB) are a group of Gram-positive, non-spore forming, cocci or rod shaped, catalase negative organisms, considered as Generally Recognized as Safe (GRAS) organisms. These bacteria are used for thousands of years for production of fermented foods beca...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2012-08-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-55-en.pdf |
| _version_ | 1797679707587608576 |
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| author | Frazaneh Tafvizi Maryam Tajabadi Ebrahimi Leila Khojare |
| author_facet | Frazaneh Tafvizi Maryam Tajabadi Ebrahimi Leila Khojare |
| author_sort | Frazaneh Tafvizi |
| collection | DOAJ |
| description | Background & Objective: Lactic acid bacteria (LAB) are a group of Gram-positive, non-spore forming, cocci or rod shaped, catalase negative organisms, considered as Generally Recognized as Safe (GRAS) organisms. These bacteria are used for thousands of years for production of fermented foods because of their ability to produce desirable changes in taste, flavor and texture. Different antimicrobial molecules such as bacteriocins produced by these bacteria that can inhibit food pathogens, so enhancing the shelf life and improving the safety of food products. Because of important role of LAB to improving the human health, molecular identification and phylogenic analysis of these bacteria based on 16S rRNA sequencing play the critical role in investigation of local sources of LAB in Iran. Materials & Methods: 5 isolates were selected from 20 isolates for molecular identification. These strains produced the high level of bacteriocin. Total genomic DNA was extracted by lysosyme extraction protocol. PCR-mediated amplification was carried out by degenerate primers. Sequencing was performed after purification of PCR product. Results: Isolates were deposited as novel strains of Lactobacillus casei and Entrococcus facium in GenBank. Conclusion: Because of high potential of local probiotic bacteria in Iran, these strains may be useful and could be used in the food industry. |
| first_indexed | 2024-03-11T23:18:47Z |
| format | Article |
| id | doaj.art-9e150efcb1fc4b2fb58db1a3e8786aa8 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:18:47Z |
| publishDate | 2012-08-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-9e150efcb1fc4b2fb58db1a3e8786aa82023-09-20T21:15:21ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232012-08-01228490Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and FoodFrazaneh Tafvizi0Maryam Tajabadi Ebrahimi1Leila Khojare2 Background & Objective: Lactic acid bacteria (LAB) are a group of Gram-positive, non-spore forming, cocci or rod shaped, catalase negative organisms, considered as Generally Recognized as Safe (GRAS) organisms. These bacteria are used for thousands of years for production of fermented foods because of their ability to produce desirable changes in taste, flavor and texture. Different antimicrobial molecules such as bacteriocins produced by these bacteria that can inhibit food pathogens, so enhancing the shelf life and improving the safety of food products. Because of important role of LAB to improving the human health, molecular identification and phylogenic analysis of these bacteria based on 16S rRNA sequencing play the critical role in investigation of local sources of LAB in Iran. Materials & Methods: 5 isolates were selected from 20 isolates for molecular identification. These strains produced the high level of bacteriocin. Total genomic DNA was extracted by lysosyme extraction protocol. PCR-mediated amplification was carried out by degenerate primers. Sequencing was performed after purification of PCR product. Results: Isolates were deposited as novel strains of Lactobacillus casei and Entrococcus facium in GenBank. Conclusion: Because of high potential of local probiotic bacteria in Iran, these strains may be useful and could be used in the food industry.http://jabs.fums.ac.ir/article-1-55-en.pdflactobacillusphylogenic analysis16s rrnabacteriocin |
| spellingShingle | Frazaneh Tafvizi Maryam Tajabadi Ebrahimi Leila Khojare Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food Journal of Advanced Biomedical Sciences lactobacillus phylogenic analysis 16s rrna bacteriocin |
| title | Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food |
| title_full | Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food |
| title_fullStr | Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food |
| title_full_unstemmed | Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food |
| title_short | Genotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food |
| title_sort | genotypic and phylogenic analysis of lactobacilli producing bacteriocin isolated from traditional dairy products and food |
| topic | lactobacillus phylogenic analysis 16s rrna bacteriocin |
| url | http://jabs.fums.ac.ir/article-1-55-en.pdf |
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