Expression of NUCB2/NESF-1 in Breast Cancer Cells
Recently, the expression of NUCB2/NESF-1 has been linked to tumor development. We report NUCB2/NESF-1 expression and its relation to clinicopathological parameters in breast cancer cells. Immunohistochemical reactions were conducted on 446 cases of invasive ductal carcinoma (IDC) and 36 cases of mas...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9177 |
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| author | Alicja Kmiecik Katarzyna Ratajczak-Wielgomas Jędrzej Grzegrzółka Hanna Romanowicz Beata Smolarz Piotr Dziegiel |
| author_facet | Alicja Kmiecik Katarzyna Ratajczak-Wielgomas Jędrzej Grzegrzółka Hanna Romanowicz Beata Smolarz Piotr Dziegiel |
| author_sort | Alicja Kmiecik |
| collection | DOAJ |
| description | Recently, the expression of NUCB2/NESF-1 has been linked to tumor development. We report NUCB2/NESF-1 expression and its relation to clinicopathological parameters in breast cancer cells. Immunohistochemical reactions were conducted on 446 cases of invasive ductal carcinoma (IDC) and 36 cases of mastopathy. The expression of NUCB2/NESF-1 was also examined at the mRNA and protein levels in breast cancer cell lines. A statistically significant higher level of NUCB2/NESF-1 in IDC cells was noted compared to that in mastopathy samples. The level of NUCB2 expression in the cytoplasm of IDC cells decreased with the increasing degree of tumor malignancy (G). Higher NUCB2 expression was found in tumors with estrogen receptor (ER)-positive and progesterone receptor (PR)-positive phenotypes compared to that in estrogen-receptor-negative and progesterone-receptor-negative cases. Moreover, a higher expression was shown in ER(+) and PR(+) MCF-7 and T47D cell lines compared to that in triple-negative MDA-MB-468 and normal human breast epithelial cells. The analysis of the five-year survival rate indicated that a positive NUCB2/NESF-1 expression in tumor cells was also associated with longer patient survival. The study results suggest that NUCB2/NESF1 may play an important role in malignant transformation and may be a positive prognostic factor in IDC. |
| first_indexed | 2024-03-09T09:55:34Z |
| format | Article |
| id | doaj.art-9e18a2e3de894014b032056362dd6ae1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:55:34Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9e18a2e3de894014b032056362dd6ae12023-12-01T23:47:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316917710.3390/ijms23169177Expression of NUCB2/NESF-1 in Breast Cancer CellsAlicja Kmiecik0Katarzyna Ratajczak-Wielgomas1Jędrzej Grzegrzółka2Hanna Romanowicz3Beata Smolarz4Piotr Dziegiel5Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDepartment of Pathology, Polish Mother Memorial Hospital-Research Institute, 93-338 Lodz, PolandDepartment of Pathology, Polish Mother Memorial Hospital-Research Institute, 93-338 Lodz, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandRecently, the expression of NUCB2/NESF-1 has been linked to tumor development. We report NUCB2/NESF-1 expression and its relation to clinicopathological parameters in breast cancer cells. Immunohistochemical reactions were conducted on 446 cases of invasive ductal carcinoma (IDC) and 36 cases of mastopathy. The expression of NUCB2/NESF-1 was also examined at the mRNA and protein levels in breast cancer cell lines. A statistically significant higher level of NUCB2/NESF-1 in IDC cells was noted compared to that in mastopathy samples. The level of NUCB2 expression in the cytoplasm of IDC cells decreased with the increasing degree of tumor malignancy (G). Higher NUCB2 expression was found in tumors with estrogen receptor (ER)-positive and progesterone receptor (PR)-positive phenotypes compared to that in estrogen-receptor-negative and progesterone-receptor-negative cases. Moreover, a higher expression was shown in ER(+) and PR(+) MCF-7 and T47D cell lines compared to that in triple-negative MDA-MB-468 and normal human breast epithelial cells. The analysis of the five-year survival rate indicated that a positive NUCB2/NESF-1 expression in tumor cells was also associated with longer patient survival. The study results suggest that NUCB2/NESF1 may play an important role in malignant transformation and may be a positive prognostic factor in IDC.https://www.mdpi.com/1422-0067/23/16/9177nesfatin-1nucleobindin-2 (NUCB2)breast cancerprognostic factors |
| spellingShingle | Alicja Kmiecik Katarzyna Ratajczak-Wielgomas Jędrzej Grzegrzółka Hanna Romanowicz Beata Smolarz Piotr Dziegiel Expression of NUCB2/NESF-1 in Breast Cancer Cells International Journal of Molecular Sciences nesfatin-1 nucleobindin-2 (NUCB2) breast cancer prognostic factors |
| title | Expression of NUCB2/NESF-1 in Breast Cancer Cells |
| title_full | Expression of NUCB2/NESF-1 in Breast Cancer Cells |
| title_fullStr | Expression of NUCB2/NESF-1 in Breast Cancer Cells |
| title_full_unstemmed | Expression of NUCB2/NESF-1 in Breast Cancer Cells |
| title_short | Expression of NUCB2/NESF-1 in Breast Cancer Cells |
| title_sort | expression of nucb2 nesf 1 in breast cancer cells |
| topic | nesfatin-1 nucleobindin-2 (NUCB2) breast cancer prognostic factors |
| url | https://www.mdpi.com/1422-0067/23/16/9177 |
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