Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice
Varicella–zoster virus (VZV) is the causative agent of varicella and herpes zoster (HZ) and can pose a significant challenge to human health globally. The initial VZV infection—more common in children—causes a self-limiting chicken pox. However, in later life, the latent VZV can become reactivated i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1785 |
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| author | Wenhui Xue Tingting Li Sibo Zhang Yingbin Wang Minqing Hong Lingyan Cui Hong Wang Yuyun Zhang Tingting Chen Rui Zhu Zhenqin Chen Lizhi Zhou Rongwei Zhang Tong Cheng Qingbing Zheng Jun Zhang Ying Gu Ningshao Xia Shaowei Li |
| author_facet | Wenhui Xue Tingting Li Sibo Zhang Yingbin Wang Minqing Hong Lingyan Cui Hong Wang Yuyun Zhang Tingting Chen Rui Zhu Zhenqin Chen Lizhi Zhou Rongwei Zhang Tong Cheng Qingbing Zheng Jun Zhang Ying Gu Ningshao Xia Shaowei Li |
| author_sort | Wenhui Xue |
| collection | DOAJ |
| description | Varicella–zoster virus (VZV) is the causative agent of varicella and herpes zoster (HZ) and can pose a significant challenge to human health globally. The initial VZV infection—more common in children—causes a self-limiting chicken pox. However, in later life, the latent VZV can become reactivated in these patients, causing HZ and postherpetic neuralgia (PHN), a serious and painful complication. VZV glycoprotein E (gE) has been developed into a licensed subunit vaccine against HZ (Shingrix). However, its efficacy relies on the concomitant delivery of a robust adjuvant (AS01B). Here, we sought to create a new immunogen for vaccine design by displaying the VZV–gE on the baculovirus surface (Bac–gE). Correct localization and display of gE on the engineered baculovirus was verified by flow cytometry and immune electron microscopy. We show that Bac–gE provides excellent antigenicity against VZV and induces not only stronger gE-specific CD4<sup>+</sup> and CD8<sup>+</sup> T cell responses but also higher levels of VZV–specific neutralizing antibodies as compared with other vaccine strategies in mice. Collectively, we show that the baculovirus display of VZV–gE confers ideal humoral and cellular immune responses required for HZ vaccine development, paving the way for a baculovirus-based vaccine design. |
| first_indexed | 2024-03-09T09:48:02Z |
| format | Article |
| id | doaj.art-9e1b8d88d0b848059d923e8929c9828b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T09:48:02Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-9e1b8d88d0b848059d923e8929c9828b2023-12-02T00:26:53ZengMDPI AGViruses1999-49152022-08-01148178510.3390/v14081785Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in MiceWenhui Xue0Tingting Li1Sibo Zhang2Yingbin Wang3Minqing Hong4Lingyan Cui5Hong Wang6Yuyun Zhang7Tingting Chen8Rui Zhu9Zhenqin Chen10Lizhi Zhou11Rongwei Zhang12Tong Cheng13Qingbing Zheng14Jun Zhang15Ying Gu16Ningshao Xia17Shaowei Li18State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, ChinaVaricella–zoster virus (VZV) is the causative agent of varicella and herpes zoster (HZ) and can pose a significant challenge to human health globally. The initial VZV infection—more common in children—causes a self-limiting chicken pox. However, in later life, the latent VZV can become reactivated in these patients, causing HZ and postherpetic neuralgia (PHN), a serious and painful complication. VZV glycoprotein E (gE) has been developed into a licensed subunit vaccine against HZ (Shingrix). However, its efficacy relies on the concomitant delivery of a robust adjuvant (AS01B). Here, we sought to create a new immunogen for vaccine design by displaying the VZV–gE on the baculovirus surface (Bac–gE). Correct localization and display of gE on the engineered baculovirus was verified by flow cytometry and immune electron microscopy. We show that Bac–gE provides excellent antigenicity against VZV and induces not only stronger gE-specific CD4<sup>+</sup> and CD8<sup>+</sup> T cell responses but also higher levels of VZV–specific neutralizing antibodies as compared with other vaccine strategies in mice. Collectively, we show that the baculovirus display of VZV–gE confers ideal humoral and cellular immune responses required for HZ vaccine development, paving the way for a baculovirus-based vaccine design.https://www.mdpi.com/1999-4915/14/8/1785Varicella Zoster Virus (VZV)HZ vaccineglycoprotein Ebaculovirus displaycellular immunity |
| spellingShingle | Wenhui Xue Tingting Li Sibo Zhang Yingbin Wang Minqing Hong Lingyan Cui Hong Wang Yuyun Zhang Tingting Chen Rui Zhu Zhenqin Chen Lizhi Zhou Rongwei Zhang Tong Cheng Qingbing Zheng Jun Zhang Ying Gu Ningshao Xia Shaowei Li Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice Viruses Varicella Zoster Virus (VZV) HZ vaccine glycoprotein E baculovirus display cellular immunity |
| title | Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice |
| title_full | Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice |
| title_fullStr | Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice |
| title_full_unstemmed | Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice |
| title_short | Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice |
| title_sort | baculovirus display of varicella zoster virus glycoprotein e induces robust humoral and cellular immune responses in mice |
| topic | Varicella Zoster Virus (VZV) HZ vaccine glycoprotein E baculovirus display cellular immunity |
| url | https://www.mdpi.com/1999-4915/14/8/1785 |
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