Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia
Background: Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.Objective: To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.Methods: Blo...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2023-12-01
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| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | https://iji.sums.ac.ir/article_49660_91f45b07b9349fd7c6a3c340a28ed3f1.pdf |
| _version_ | 1827394703156314112 |
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| author | Maryam Mohammadi Hossein Asgarian-Omran Behnam Najafi Ahmad Najafi Reza Valadan Hossein Karami Mohammad Naderisoraki Maryam Alizadeforutan Ramin Shekarriz Mohsen Tehrani |
| author_facet | Maryam Mohammadi Hossein Asgarian-Omran Behnam Najafi Ahmad Najafi Reza Valadan Hossein Karami Mohammad Naderisoraki Maryam Alizadeforutan Ramin Shekarriz Mohsen Tehrani |
| author_sort | Maryam Mohammadi |
| collection | DOAJ |
| description | Background: Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.Objective: To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.Methods: Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.Results: Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.Conclusion: NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation. |
| first_indexed | 2024-03-08T18:17:49Z |
| format | Article |
| id | doaj.art-9e1db0fc3a11477aa3f681d560a42aa6 |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-03-08T18:17:49Z |
| publishDate | 2023-12-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-9e1db0fc3a11477aa3f681d560a42aa62023-12-31T08:48:27ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2023-12-0120443844510.22034/iji.2023.97902.253749660Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute LeukemiaMaryam Mohammadi0Hossein Asgarian-Omran1Behnam Najafi2Ahmad Najafi3Reza Valadan4Hossein Karami5Mohammad Naderisoraki6Maryam Alizadeforutan7Ramin Shekarriz8Mohsen Tehrani9Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.Molecular and Cell-Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.Thalassemia Research Center (TRC), Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran.Thalassemia Research Center (TRC), Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran.Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran.Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran.Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.Background: Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.Objective: To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.Methods: Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.Results: Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.Conclusion: NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation.https://iji.sums.ac.ir/article_49660_91f45b07b9349fd7c6a3c340a28ed3f1.pdfleukemianr4at cell exhaustiontox |
| spellingShingle | Maryam Mohammadi Hossein Asgarian-Omran Behnam Najafi Ahmad Najafi Reza Valadan Hossein Karami Mohammad Naderisoraki Maryam Alizadeforutan Ramin Shekarriz Mohsen Tehrani Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia Iranian Journal of Immunology leukemia nr4a t cell exhaustion tox |
| title | Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia |
| title_full | Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia |
| title_fullStr | Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia |
| title_full_unstemmed | Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia |
| title_short | Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia |
| title_sort | evaluation of mrna expressions of tox and nr4as in cd8 t cells in acute leukemia |
| topic | leukemia nr4a t cell exhaustion tox |
| url | https://iji.sums.ac.ir/article_49660_91f45b07b9349fd7c6a3c340a28ed3f1.pdf |
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