Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS
Huijuan Chen,1 Xinhua Bai,2 Yang Gao,2 Wenxuan Liu,2 Xuena Yao,2 Jing Wang2 1Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, People’s Republic of China; 2Department of Clinical Laboratory, Beijing Capitalbio Medlab, Beijing...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-11-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/profile-of-bacteria-with-args-among-real-world-samples-from-icu-admiss-peer-reviewed-fulltext-article-IDR |
| _version_ | 1818750980492099584 |
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| author | Chen H Bai X Gao Y Liu W Yao X Wang J |
| author_facet | Chen H Bai X Gao Y Liu W Yao X Wang J |
| author_sort | Chen H |
| collection | DOAJ |
| description | Huijuan Chen,1 Xinhua Bai,2 Yang Gao,2 Wenxuan Liu,2 Xuena Yao,2 Jing Wang2 1Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, People’s Republic of China; 2Department of Clinical Laboratory, Beijing Capitalbio Medlab, Beijing, People’s Republic of ChinaCorrespondence: Huijuan Chen Tel +86-10-8722-9999Email m13810539097@163.comBackground: Treatment of pulmonary infections in the intensive care unit (ICU) represents a great challenge, especially infections caused by antibiotic resistance pathogens. A thorough and up-to-date knowledge of the local spectrum of antibiotic resistant bacteria can improve the antibiotic treatment efficiency. In this study, we aimed to reveal the profile of bacteria with antibiotic resistance genes (ARGs) in real-world samples from ICU admission patients with pulmonary infection in Mainland, China, by metagenomic next-generation sequencing (mNGS).Methods: A total of 504 different types of clinical samples from 452 ICU admission patients with pulmonary infection were detected by mNGS analysis.Results: A total of 485 samples from 434 patients got successful mNGS results. Among 434 patients, one or more bacteria with ARGs were detected in 192 patients (44.24%, 192/434), and ≥ 2 bacteria with ARGs were detected in 85 (19.59%, 85/434) patients. The predominant detected bacteria were Corynebacterium striatum (C. striatum) (11.76%, 51/434), Acinetobacter baumannii (A. baumannii) (11.52%, 50/434) and Enterococcus faecium (E. faecium) (8.99%, 39/434). ermX conferred resistance to MSLB and cmx to phenicol were the only two ARGs detected in C. striatum; in A. baumannii, most of ARGs were resistance-nodulation-division (RND)-type efflux pumps genes, which conferred resistance to multi-drug; ermB conferred resistance to MSLB and efmA to multi-drug were the predominant ARGs in E. faecium. Bacteria with ARGs were detected in 50% (140/280) bronchoalveolar lavage fluid (BALF) and 50.5% (48/95) sputum samples, which were significantly higher than in blood and cerebrospinal fluid (CSF) samples.Conclusion: High level of bacteria with ARGs was observed in clinical samples, especially BALF and sputum samples from ICU admission patients with pulmonary infection in Mainland, China. And C. striatum resistant to MSLB and/or phenicol, multi-drug resistance A. baumannii and E. faecium were the lead bacteria.Keywords: intensive care units, pulmonary infection, antibiotic resistant bacteria, antibiotic resistance genes |
| first_indexed | 2024-12-18T04:28:18Z |
| format | Article |
| id | doaj.art-9e1e989ffcc9478e9783b90a0f39c090 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-18T04:28:18Z |
| publishDate | 2021-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-9e1e989ffcc9478e9783b90a0f39c0902022-12-21T21:21:02ZengDove Medical PressInfection and Drug Resistance1178-69732021-11-01Volume 144993500470959Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGSChen HBai XGao YLiu WYao XWang JHuijuan Chen,1 Xinhua Bai,2 Yang Gao,2 Wenxuan Liu,2 Xuena Yao,2 Jing Wang2 1Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, People’s Republic of China; 2Department of Clinical Laboratory, Beijing Capitalbio Medlab, Beijing, People’s Republic of ChinaCorrespondence: Huijuan Chen Tel +86-10-8722-9999Email m13810539097@163.comBackground: Treatment of pulmonary infections in the intensive care unit (ICU) represents a great challenge, especially infections caused by antibiotic resistance pathogens. A thorough and up-to-date knowledge of the local spectrum of antibiotic resistant bacteria can improve the antibiotic treatment efficiency. In this study, we aimed to reveal the profile of bacteria with antibiotic resistance genes (ARGs) in real-world samples from ICU admission patients with pulmonary infection in Mainland, China, by metagenomic next-generation sequencing (mNGS).Methods: A total of 504 different types of clinical samples from 452 ICU admission patients with pulmonary infection were detected by mNGS analysis.Results: A total of 485 samples from 434 patients got successful mNGS results. Among 434 patients, one or more bacteria with ARGs were detected in 192 patients (44.24%, 192/434), and ≥ 2 bacteria with ARGs were detected in 85 (19.59%, 85/434) patients. The predominant detected bacteria were Corynebacterium striatum (C. striatum) (11.76%, 51/434), Acinetobacter baumannii (A. baumannii) (11.52%, 50/434) and Enterococcus faecium (E. faecium) (8.99%, 39/434). ermX conferred resistance to MSLB and cmx to phenicol were the only two ARGs detected in C. striatum; in A. baumannii, most of ARGs were resistance-nodulation-division (RND)-type efflux pumps genes, which conferred resistance to multi-drug; ermB conferred resistance to MSLB and efmA to multi-drug were the predominant ARGs in E. faecium. Bacteria with ARGs were detected in 50% (140/280) bronchoalveolar lavage fluid (BALF) and 50.5% (48/95) sputum samples, which were significantly higher than in blood and cerebrospinal fluid (CSF) samples.Conclusion: High level of bacteria with ARGs was observed in clinical samples, especially BALF and sputum samples from ICU admission patients with pulmonary infection in Mainland, China. And C. striatum resistant to MSLB and/or phenicol, multi-drug resistance A. baumannii and E. faecium were the lead bacteria.Keywords: intensive care units, pulmonary infection, antibiotic resistant bacteria, antibiotic resistance geneshttps://www.dovepress.com/profile-of-bacteria-with-args-among-real-world-samples-from-icu-admiss-peer-reviewed-fulltext-article-IDRintensive care unitspulmonary infectionantibiotic resistant bacteriaantibiotic resistance genes |
| spellingShingle | Chen H Bai X Gao Y Liu W Yao X Wang J Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS Infection and Drug Resistance intensive care units pulmonary infection antibiotic resistant bacteria antibiotic resistance genes |
| title | Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS |
| title_full | Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS |
| title_fullStr | Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS |
| title_full_unstemmed | Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS |
| title_short | Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS |
| title_sort | profile of bacteria with args among real world samples from icu admission patients with pulmonary infection revealed by metagenomic ngs |
| topic | intensive care units pulmonary infection antibiotic resistant bacteria antibiotic resistance genes |
| url | https://www.dovepress.com/profile-of-bacteria-with-args-among-real-world-samples-from-icu-admiss-peer-reviewed-fulltext-article-IDR |
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