Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment
Practical application of the partial nitritation–anaerobic ammonium oxidation (anammox) process has attracted increasing attention because of its low operational costs. However, the nitritation process, as a promising way to supply nitrite for anammox, is sensitive to the variations in substrate con...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.851565/full |
| _version_ | 1818776505200672768 |
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| author | Kuo Zhang Xinjue Li Shou-Qing Ni Sitong Liu |
| author_facet | Kuo Zhang Xinjue Li Shou-Qing Ni Sitong Liu |
| author_sort | Kuo Zhang |
| collection | DOAJ |
| description | Practical application of the partial nitritation–anaerobic ammonium oxidation (anammox) process has attracted increasing attention because of its low operational costs. However, the nitritation process, as a promising way to supply nitrite for anammox, is sensitive to the variations in substrate concentration and dissolved oxygen (DO) concentration. Therefore, a stable supply of nitrite becomes a real bottleneck in partial nitritation–anammox process, limiting their potential for application in mainstream wastewater treatment. In this study, five 18-L sequencing batch reactors were operated in parallel at room temperature (22°C ± 4°C) to explore the nitritation performance with different carrier materials, including sepiolite-nonwoven carrier (R1), zeolite-nonwoven carrier (R2), brucite-nonwoven carrier (R3), polyurethane carrier (R4), and nonwoven carrier (R5). The ammonia oxidation rate (AOR) in R1 reached the highest level of 0.174 g-N L−1 d−1 in phase II, which was 1.4-fold higher than the control reactor (R4). To guarantee a stable supply of nitrite for anammox process, the nitrite accumulation efficiency (NAE) was always higher than 77%, even though the free ammonia (FA) decreases to 0.08 mg-N/L, and the pH decreases to 6.8 ± 0.3. In phase V, the AOR in R1 reached 0.206 g-N L−1 d−1 after the DO content increase from 0.7 ± 0.3 mg/L to 1.7 ± 0.3 mg/L. The NAE in R1 was consistently higher than 68.6%, which was much higher than the other reactor systems (R2: 43.8%, R3: 46.6%, R4: 23.7%, R5: 22.7%). Analysis of 16S rRNA gene sequencing revealed that the relative abundance of Nitrobacter and Nitrospira in R1 was significantly lower than other reactors, indicating that the sepiolite carrier plays an important role in the inhibition of nitrite-oxidizing bacteria. These results indicate that the sepiolite nonwoven composite carrier can effectively improve the nitritation process, which is highly beneficial for the application of partial nitritation–anammox for mainstream wastewater treatment. |
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| language | English |
| last_indexed | 2024-12-18T11:14:00Z |
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| spelling | doaj.art-9e1fa38148c345fe91149b998053b0a92022-12-21T21:09:57ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.851565851565Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater TreatmentKuo Zhang0Xinjue Li1Shou-Qing Ni2Sitong Liu3Department of Environmental Sciences and Engineering, College of Environmental Sciences and Engineering, Peking University, Beijing, ChinaDepartment of Environmental Sciences and Engineering, College of Environmental Sciences and Engineering, Peking University, Beijing, ChinaShandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, ChinaDepartment of Environmental Sciences and Engineering, College of Environmental Sciences and Engineering, Peking University, Beijing, ChinaPractical application of the partial nitritation–anaerobic ammonium oxidation (anammox) process has attracted increasing attention because of its low operational costs. However, the nitritation process, as a promising way to supply nitrite for anammox, is sensitive to the variations in substrate concentration and dissolved oxygen (DO) concentration. Therefore, a stable supply of nitrite becomes a real bottleneck in partial nitritation–anammox process, limiting their potential for application in mainstream wastewater treatment. In this study, five 18-L sequencing batch reactors were operated in parallel at room temperature (22°C ± 4°C) to explore the nitritation performance with different carrier materials, including sepiolite-nonwoven carrier (R1), zeolite-nonwoven carrier (R2), brucite-nonwoven carrier (R3), polyurethane carrier (R4), and nonwoven carrier (R5). The ammonia oxidation rate (AOR) in R1 reached the highest level of 0.174 g-N L−1 d−1 in phase II, which was 1.4-fold higher than the control reactor (R4). To guarantee a stable supply of nitrite for anammox process, the nitrite accumulation efficiency (NAE) was always higher than 77%, even though the free ammonia (FA) decreases to 0.08 mg-N/L, and the pH decreases to 6.8 ± 0.3. In phase V, the AOR in R1 reached 0.206 g-N L−1 d−1 after the DO content increase from 0.7 ± 0.3 mg/L to 1.7 ± 0.3 mg/L. The NAE in R1 was consistently higher than 68.6%, which was much higher than the other reactor systems (R2: 43.8%, R3: 46.6%, R4: 23.7%, R5: 22.7%). Analysis of 16S rRNA gene sequencing revealed that the relative abundance of Nitrobacter and Nitrospira in R1 was significantly lower than other reactors, indicating that the sepiolite carrier plays an important role in the inhibition of nitrite-oxidizing bacteria. These results indicate that the sepiolite nonwoven composite carrier can effectively improve the nitritation process, which is highly beneficial for the application of partial nitritation–anammox for mainstream wastewater treatment.https://www.frontiersin.org/articles/10.3389/fbioe.2022.851565/fullnitritationanammoxcomposite carriersepiolitenitrogen removal |
| spellingShingle | Kuo Zhang Xinjue Li Shou-Qing Ni Sitong Liu Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment Frontiers in Bioengineering and Biotechnology nitritation anammox composite carrier sepiolite nitrogen removal |
| title | Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment |
| title_full | Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment |
| title_fullStr | Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment |
| title_full_unstemmed | Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment |
| title_short | Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment |
| title_sort | comparison of different carriers to maintain a stable partial nitrification process for low strength wastewater treatment |
| topic | nitritation anammox composite carrier sepiolite nitrogen removal |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.851565/full |
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