Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration
Peroxiredoxin 5 (Prdx5) is involved in pathophysiological regulation via the stress-induced cellular response. However, its function in the bone remains largely unknown. Here, we show that Prdx5 is involved in osteoclast and osteoblast differentiation, resulting in osteoporotic phenotypes in Prdx5 k...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/80122 |
| _version_ | 1811172893020651520 |
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| author | Eunjin Cho Xiangguo Che Mary Jasmin Ang Seongmin Cheon Jinkyung Lee Kwang Soo Kim Chang Hoon Lee Sang-Yeop Lee Hee-Young Yang Changjong Moon Chungoo Park Je-Yong Choi Tae-Hoon Lee |
| author_facet | Eunjin Cho Xiangguo Che Mary Jasmin Ang Seongmin Cheon Jinkyung Lee Kwang Soo Kim Chang Hoon Lee Sang-Yeop Lee Hee-Young Yang Changjong Moon Chungoo Park Je-Yong Choi Tae-Hoon Lee |
| author_sort | Eunjin Cho |
| collection | DOAJ |
| description | Peroxiredoxin 5 (Prdx5) is involved in pathophysiological regulation via the stress-induced cellular response. However, its function in the bone remains largely unknown. Here, we show that Prdx5 is involved in osteoclast and osteoblast differentiation, resulting in osteoporotic phenotypes in Prdx5 knockout (Prdx5Ko) male mice. To investigate the function of Prdx5 in the bone, osteoblasts were analyzed through immunoprecipitation (IP) and liquid chromatography combined with tandem mass spectrometry (LC–MS/MS) methods, while osteoclasts were analyzed through RNA-sequencing. Heterogeneous nuclear ribonucleoprotein K (hnRNPK) was identified as a potential binding partner of Prdx5 during osteoblast differentiation in vitro. Prdx5 acts as a negative regulator of hnRNPK-mediated osteocalcin (Bglap) expression. In addition, transcriptomic analysis revealed that in vitro differentiated osteoclasts from the bone marrow-derived macrophages of Prdx5Ko mice showed enhanced expression of several osteoclast-related genes. These findings indicate that Prdx5 might contribute to the maintenance of bone homeostasis by regulating osteoblast differentiation. This study proposes a new function of Prdx5 in bone remodeling that may be used in developing therapeutic strategies for bone diseases. |
| first_indexed | 2024-04-10T17:39:09Z |
| format | Article |
| id | doaj.art-9e249cd3af0444ff9034a2e0ea354e8f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-10T17:39:09Z |
| publishDate | 2023-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9e249cd3af0444ff9034a2e0ea354e8f2023-02-03T16:33:09ZengeLife Sciences Publications LtdeLife2050-084X2023-02-011210.7554/eLife.80122Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regenerationEunjin Cho0https://orcid.org/0000-0003-0276-4728Xiangguo Che1Mary Jasmin Ang2Seongmin Cheon3Jinkyung Lee4Kwang Soo Kim5Chang Hoon Lee6https://orcid.org/0000-0001-8953-9069Sang-Yeop Lee7Hee-Young Yang8Changjong Moon9https://orcid.org/0000-0003-2451-0374Chungoo Park10Je-Yong Choi11Tae-Hoon Lee12https://orcid.org/0000-0003-0105-1750Department of Oral Biochemistry, Korea Mouse Phenotype Center (KMPC), Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of KoreaDepartment of Biochemistry and Cell Biology, BK21 Plus KNU Biomedical Convergence Program, Skeletal Diseases Analysis Center, Korea Mouse Phenotyping Center (KMPC), School of Medicine, Kyungpook National University, Daegu, Republic of KoreaDepartment of Basic Veterinary Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Los Baños, PhilippinesSchool of Biological Sciences and Technology, Chonnam National University, Gwangju, Republic of Korea; Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of KoreaDepartment of Oral Biochemistry, Korea Mouse Phenotype Center (KMPC), Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of KoreaDepartment of Microbiology, Department of Molecular Medicine (BK21plus), Chonnam National University Medical School, Gwangju, Republic of KoreaTherapeutic & Biotechnology Division, Drug Discovery Platform Research Center, Research Institute of Chemical Technology (KRICT), Daejeon, Republic of KoreaResearch Center for Bioconvergence Analysis, Korea Basic Science Institute, Ochang, Republic of KoreaPreclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Republic of KoreaDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju, Republic of KoreaSchool of Biological Sciences and Technology, Chonnam National University, Gwangju, Republic of KoreaDepartment of Biochemistry and Cell Biology, BK21 Plus KNU Biomedical Convergence Program, Skeletal Diseases Analysis Center, Korea Mouse Phenotyping Center (KMPC), School of Medicine, Kyungpook National University, Daegu, Republic of KoreaDepartment of Oral Biochemistry, Korea Mouse Phenotype Center (KMPC), Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of KoreaPeroxiredoxin 5 (Prdx5) is involved in pathophysiological regulation via the stress-induced cellular response. However, its function in the bone remains largely unknown. Here, we show that Prdx5 is involved in osteoclast and osteoblast differentiation, resulting in osteoporotic phenotypes in Prdx5 knockout (Prdx5Ko) male mice. To investigate the function of Prdx5 in the bone, osteoblasts were analyzed through immunoprecipitation (IP) and liquid chromatography combined with tandem mass spectrometry (LC–MS/MS) methods, while osteoclasts were analyzed through RNA-sequencing. Heterogeneous nuclear ribonucleoprotein K (hnRNPK) was identified as a potential binding partner of Prdx5 during osteoblast differentiation in vitro. Prdx5 acts as a negative regulator of hnRNPK-mediated osteocalcin (Bglap) expression. In addition, transcriptomic analysis revealed that in vitro differentiated osteoclasts from the bone marrow-derived macrophages of Prdx5Ko mice showed enhanced expression of several osteoclast-related genes. These findings indicate that Prdx5 might contribute to the maintenance of bone homeostasis by regulating osteoblast differentiation. This study proposes a new function of Prdx5 in bone remodeling that may be used in developing therapeutic strategies for bone diseases.https://elifesciences.org/articles/80122peroxiredoxinosteoblastosteoclastosteoporosisbone regeneration |
| spellingShingle | Eunjin Cho Xiangguo Che Mary Jasmin Ang Seongmin Cheon Jinkyung Lee Kwang Soo Kim Chang Hoon Lee Sang-Yeop Lee Hee-Young Yang Changjong Moon Chungoo Park Je-Yong Choi Tae-Hoon Lee Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration eLife peroxiredoxin osteoblast osteoclast osteoporosis bone regeneration |
| title | Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration |
| title_full | Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration |
| title_fullStr | Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration |
| title_full_unstemmed | Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration |
| title_short | Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration |
| title_sort | peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnrnpk during bone regeneration |
| topic | peroxiredoxin osteoblast osteoclast osteoporosis bone regeneration |
| url | https://elifesciences.org/articles/80122 |
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