SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos
We evaluated structural modifications in the branchial architecture of grass carp, Ctenopharyngodon idella, chronically exposed to chlorpyrifos (an organophosphate) using scanning electron microscopy (SEM). Static renewal tests were conducted for 96 h to determine the LC50 of chlorpyrifos t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600034K.pdf |
| Summary: | We evaluated structural modifications in the branchial architecture of grass
carp, Ctenopharyngodon idella, chronically exposed to chlorpyrifos (an
organophosphate) using scanning electron microscopy (SEM). Static renewal
tests were conducted for 96 h to determine the LC50 of chlorpyrifos to the
fish. Physicochemical analysis of water was done using standard methods. To
assess the effect of chronic toxicity, fish were exposed to two sublethal
concentrations (1.44 μg/L and 2.41 μg/L) of chlorpyrifos for 15, 30 and 60
days, after which gills were examined by SEM, which revealed changes in gill
ultrastructure. Branchial alterations included distorted secondary lamellae
in the form of curling and shortening, erosion in a few primary filaments and
a wrinkled and denuded epithelial surface. Excessive mucosal openings (mucoid
hyperplasia) on the surface were observed in the gills of fish exposed to
both concentrations of chlorpyrifos. Alteration in the microridge pattern of
pavement cells and cracks on the gill rakers were also observed, and the
intensity of the damage was found to be directly related to the toxicant
concentration and exposure period. The present study revealed that the
assessment of surface morphology can serve as a novel bioindicator of
pollution, disease and toxicity. |
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| ISSN: | 0354-4664 1821-4339 |