SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos
We evaluated structural modifications in the branchial architecture of grass carp, Ctenopharyngodon idella, chronically exposed to chlorpyrifos (an organophosphate) using scanning electron microscopy (SEM). Static renewal tests were conducted for 96 h to determine the LC50 of chlorpyrifos t...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600034K.pdf |
| _version_ | 1818542345458548736 |
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| author | Kaur Mandeep Jindal Rajinder |
| author_facet | Kaur Mandeep Jindal Rajinder |
| author_sort | Kaur Mandeep |
| collection | DOAJ |
| description | We evaluated structural modifications in the branchial architecture of grass
carp, Ctenopharyngodon idella, chronically exposed to chlorpyrifos (an
organophosphate) using scanning electron microscopy (SEM). Static renewal
tests were conducted for 96 h to determine the LC50 of chlorpyrifos to the
fish. Physicochemical analysis of water was done using standard methods. To
assess the effect of chronic toxicity, fish were exposed to two sublethal
concentrations (1.44 μg/L and 2.41 μg/L) of chlorpyrifos for 15, 30 and 60
days, after which gills were examined by SEM, which revealed changes in gill
ultrastructure. Branchial alterations included distorted secondary lamellae
in the form of curling and shortening, erosion in a few primary filaments and
a wrinkled and denuded epithelial surface. Excessive mucosal openings (mucoid
hyperplasia) on the surface were observed in the gills of fish exposed to
both concentrations of chlorpyrifos. Alteration in the microridge pattern of
pavement cells and cracks on the gill rakers were also observed, and the
intensity of the damage was found to be directly related to the toxicant
concentration and exposure period. The present study revealed that the
assessment of surface morphology can serve as a novel bioindicator of
pollution, disease and toxicity. |
| first_indexed | 2024-12-11T22:20:51Z |
| format | Article |
| id | doaj.art-9e249db8f7824ad8a704e7858e59c3a4 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-11T22:20:51Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-9e249db8f7824ad8a704e7858e59c3a42022-12-22T00:48:27ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168239339810.2298/ABS150701034K0354-46641600034KSEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifosKaur Mandeep0Jindal Rajinder1Panjab University, Department of Zoology, Aquatic Biology Laboratory, Chandigarh, IndiaPanjab University, Department of Zoology, Aquatic Biology Laboratory, Chandigarh, IndiaWe evaluated structural modifications in the branchial architecture of grass carp, Ctenopharyngodon idella, chronically exposed to chlorpyrifos (an organophosphate) using scanning electron microscopy (SEM). Static renewal tests were conducted for 96 h to determine the LC50 of chlorpyrifos to the fish. Physicochemical analysis of water was done using standard methods. To assess the effect of chronic toxicity, fish were exposed to two sublethal concentrations (1.44 μg/L and 2.41 μg/L) of chlorpyrifos for 15, 30 and 60 days, after which gills were examined by SEM, which revealed changes in gill ultrastructure. Branchial alterations included distorted secondary lamellae in the form of curling and shortening, erosion in a few primary filaments and a wrinkled and denuded epithelial surface. Excessive mucosal openings (mucoid hyperplasia) on the surface were observed in the gills of fish exposed to both concentrations of chlorpyrifos. Alteration in the microridge pattern of pavement cells and cracks on the gill rakers were also observed, and the intensity of the damage was found to be directly related to the toxicant concentration and exposure period. The present study revealed that the assessment of surface morphology can serve as a novel bioindicator of pollution, disease and toxicity.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600034K.pdfChlorpyrifosCtenopharyngodon idellagillstoxicityscanning electron microscopy |
| spellingShingle | Kaur Mandeep Jindal Rajinder SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos Archives of Biological Sciences Chlorpyrifos Ctenopharyngodon idella gills toxicity scanning electron microscopy |
| title | SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos |
| title_full | SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos |
| title_fullStr | SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos |
| title_full_unstemmed | SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos |
| title_short | SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos |
| title_sort | sem study of ultrastructural changes in branchial architecture of ctenopharyngodon idella cuvier valenciennes exposed to chlorpyrifos |
| topic | Chlorpyrifos Ctenopharyngodon idella gills toxicity scanning electron microscopy |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600034K.pdf |
| work_keys_str_mv | AT kaurmandeep semstudyofultrastructuralchangesinbranchialarchitectureofctenopharyngodonidellacuviervalenciennesexposedtochlorpyrifos AT jindalrajinder semstudyofultrastructuralchangesinbranchialarchitectureofctenopharyngodonidellacuviervalenciennesexposedtochlorpyrifos |