Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production
In a survey conducted in <i>Cannabis sativa</i> L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/9/802 |
| _version_ | 1811323045905694720 |
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| author | Lior Hadad Neta Luria Elisheva Smith Noa Sela Oded Lachman Aviv Dombrovsky |
| author_facet | Lior Hadad Neta Luria Elisheva Smith Noa Sela Oded Lachman Aviv Dombrovsky |
| author_sort | Lior Hadad |
| collection | DOAJ |
| description | In a survey conducted in <i>Cannabis sativa</i> L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the <i>Closteroviridae</i> family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85−99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly <i>Bemisia tabaci</i> Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, <i>Lactuca sativa,</i> and <i>Catharanthus roseus</i> plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV. |
| first_indexed | 2024-04-13T13:46:57Z |
| format | Article |
| id | doaj.art-9e25f1d08f024d35abdcb30355af625f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-13T13:46:57Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-9e25f1d08f024d35abdcb30355af625f2022-12-22T02:44:28ZengMDPI AGViruses1999-49152019-08-0111980210.3390/v11090802v11090802Lettuce Chlorosis Virus Disease: A New Threat to Cannabis ProductionLior Hadad0Neta Luria1Elisheva Smith2Noa Sela3Oded Lachman4Aviv Dombrovsky5Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelIn a survey conducted in <i>Cannabis sativa</i> L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the <i>Closteroviridae</i> family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85−99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly <i>Bemisia tabaci</i> Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, <i>Lactuca sativa,</i> and <i>Catharanthus roseus</i> plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV.https://www.mdpi.com/1999-4915/11/9/802<i>Closteroviridae</i><i>Crinivirus</i><i>Bemisia tabaci</i>chlorosisshoot propagation |
| spellingShingle | Lior Hadad Neta Luria Elisheva Smith Noa Sela Oded Lachman Aviv Dombrovsky Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production Viruses <i>Closteroviridae</i> <i>Crinivirus</i> <i>Bemisia tabaci</i> chlorosis shoot propagation |
| title | Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production |
| title_full | Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production |
| title_fullStr | Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production |
| title_full_unstemmed | Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production |
| title_short | Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production |
| title_sort | lettuce chlorosis virus disease a new threat to cannabis production |
| topic | <i>Closteroviridae</i> <i>Crinivirus</i> <i>Bemisia tabaci</i> chlorosis shoot propagation |
| url | https://www.mdpi.com/1999-4915/11/9/802 |
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