"SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA
Research subject. 26 patients at the age of 18 to 45 years with chronic urticaria were studied. The control group was composed of 25 healthy persons of 20 to 35 age. Research purpose was to pick up a cell-free «skin window» exudate fraction and study its pro-inflammatory cytokine values in chronic...
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| Format: | Article |
| Language: | Russian |
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The Publishing House Medicine and Enlightenment
2022-08-01
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| Series: | Medicina v Kuzbasse |
| Subjects: | |
| Online Access: | https://mednauki.ru/index.php/MK/article/view/756 |
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| author | Денис Сергеевич Загрешенко Владимир Васильевич Климов Наталья Александровна Трофименко Маргарита Сергеевна Дорофеева |
| author_facet | Денис Сергеевич Загрешенко Владимир Васильевич Климов Наталья Александровна Трофименко Маргарита Сергеевна Дорофеева |
| author_sort | Денис Сергеевич Загрешенко |
| collection | DOAJ |
| description | Research subject. 26 patients at the age of 18 to 45 years with chronic urticaria were studied. The control group was composed of 25 healthy persons of 20 to 35 age.
Research purpose was to pick up a cell-free «skin window» exudate fraction and study its pro-inflammatory cytokine values in chronic urticaria.
Methods. The method of «skin window» chamber modified by Klimov V.V. et al. (patent № 1534395 and medical technology ФС № 2010.217) has been used to evaluate cytokine IL-1β and IL-18 values in the skin. The ELISA was exploited to assess cytokine concentrations in exudates, whereas they were previously standardized using the microbiuret approach in relation to the total protein value.
Results. At the «shock organ» level, a statistical validity regarding the control increase in pro-inflammatory cytokines IL-1β and IL-18 was revealed in chronic urticaria. It can be a hidden cause behind inflammasomes as multimolecular complexes in many inflammatory cells in the skin.
Conclusions. In chronic urticaria, the study of «skin window» exudate pro-inflammatory cytokines is important for such «shock organ» as the skin that enables the assessment of inflammatory activity and may correspond to inflammasome formation. |
| first_indexed | 2024-04-11T07:26:41Z |
| format | Article |
| id | doaj.art-9e260819efbc43009ad507d2d063e2b0 |
| institution | Directory Open Access Journal |
| issn | 1819-0901 2588-0411 |
| language | Russian |
| last_indexed | 2024-04-11T07:26:41Z |
| publishDate | 2022-08-01 |
| publisher | The Publishing House Medicine and Enlightenment |
| record_format | Article |
| series | Medicina v Kuzbasse |
| spelling | doaj.art-9e260819efbc43009ad507d2d063e2b02022-12-22T04:37:04ZrusThe Publishing House Medicine and EnlightenmentMedicina v Kuzbasse1819-09012588-04112022-08-012132729678"SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIAДенис Сергеевич Загрешенко0Владимир Васильевич Климов1Наталья Александровна Трофименко2Маргарита Сергеевна Дорофеева3НГИУВ – филиал ФГБОУ ДПО РМАНПО Минздрава России, г. НовокузнецкФГБОУ ВО СибГМУ Минздрава России, г. ТомскГАУЗ НГКБ № 1, г. НовокузнецкГБУЗ ННД, г. НовокузнецкResearch subject. 26 patients at the age of 18 to 45 years with chronic urticaria were studied. The control group was composed of 25 healthy persons of 20 to 35 age. Research purpose was to pick up a cell-free «skin window» exudate fraction and study its pro-inflammatory cytokine values in chronic urticaria. Methods. The method of «skin window» chamber modified by Klimov V.V. et al. (patent № 1534395 and medical technology ФС № 2010.217) has been used to evaluate cytokine IL-1β and IL-18 values in the skin. The ELISA was exploited to assess cytokine concentrations in exudates, whereas they were previously standardized using the microbiuret approach in relation to the total protein value. Results. At the «shock organ» level, a statistical validity regarding the control increase in pro-inflammatory cytokines IL-1β and IL-18 was revealed in chronic urticaria. It can be a hidden cause behind inflammasomes as multimolecular complexes in many inflammatory cells in the skin. Conclusions. In chronic urticaria, the study of «skin window» exudate pro-inflammatory cytokines is important for such «shock organ» as the skin that enables the assessment of inflammatory activity and may correspond to inflammasome formation.https://mednauki.ru/index.php/MK/article/view/756хроническая крапивницацитокины«кожное окно»il-1βil-18 |
| spellingShingle | Денис Сергеевич Загрешенко Владимир Васильевич Климов Наталья Александровна Трофименко Маргарита Сергеевна Дорофеева "SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA Medicina v Kuzbasse хроническая крапивница цитокины «кожное окно» il-1β il-18 |
| title | "SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA |
| title_full | "SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA |
| title_fullStr | "SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA |
| title_full_unstemmed | "SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA |
| title_short | "SKIN WINDOW" EXUDATE IL-1Β AND IL-18 IN PATIENTS WITH CHRONIC URTICARIA |
| title_sort | skin window exudate il 1β and il 18 in patients with chronic urticaria |
| topic | хроническая крапивница цитокины «кожное окно» il-1β il-18 |
| url | https://mednauki.ru/index.php/MK/article/view/756 |
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