Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> Study
Ossiculoplasty is a surgical operation performed to restore auditory transmission through the reconstruction of the ossicular chain using prosthetics. Tissue bioengineering has assumed a pivotal role in implementing alternatives to conventional ossicular middle ear replacement prostheses, to overcom...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/12/561 |
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| author | Mario Mosconi Elena Carlotto Laura Caliogna Micaela Berni Giulia Gastaldi Michele Conti Alice Maria Brancato Valentina Bina Domenico Minervini Stefano Malpede Anna Chiara Stellato Francesco Lazzerini Luca Bruschini Marco Benazzo Pietro Canzi |
| author_facet | Mario Mosconi Elena Carlotto Laura Caliogna Micaela Berni Giulia Gastaldi Michele Conti Alice Maria Brancato Valentina Bina Domenico Minervini Stefano Malpede Anna Chiara Stellato Francesco Lazzerini Luca Bruschini Marco Benazzo Pietro Canzi |
| author_sort | Mario Mosconi |
| collection | DOAJ |
| description | Ossiculoplasty is a surgical operation performed to restore auditory transmission through the reconstruction of the ossicular chain using prosthetics. Tissue bioengineering has assumed a pivotal role in implementing alternatives to conventional ossicular middle ear replacement prostheses, to overcome extrusion while preserving acoustic properties. This <i>in vitro</i> study aims to explore, for the first time in current literature, the feasibility of a biohybrid middle ear prosthesis, composed of titanium surrounded by a bone extracellular matrix as bio-coating. We have hereby studied the adhesion and proliferation of human adipose-derived mesenchymal stem cells (hASC) on titanium scaffolds <i>in vitro</i>. Moreover, we identified the osteogenic differentiation of hASC using an immunofluorescence assay to analyze osteoblasts’ gene expression profiles (Alp, Runx2, Col1a1, Osx, and Bglap), and we counted the presence of collagen as a marker of hASC’s ability to secrete an extracellular matrix. We utilized scanning electron microscopy to evaluate the presence of an extracellular matrix on the scaffolds. Our preliminary data demonstrated the titanium’s ability to support human adipose-derived mesenchymal stem cell colonization, proliferation, and osteoblastic differentiation, in order to obtain a biohybrid device. Our experience seems encouraging; thus, we advocate for further <i>in vivo</i> research to corroborate our results regarding bone transplantation. |
| first_indexed | 2024-03-08T20:39:04Z |
| format | Article |
| id | doaj.art-9e2697c3c8694af991e0bf462ad24088 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-08T20:39:04Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-9e2697c3c8694af991e0bf462ad240882023-12-22T14:17:59ZengMDPI AGJournal of Functional Biomaterials2079-49832023-11-01141256110.3390/jfb14120561Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> StudyMario Mosconi0Elena Carlotto1Laura Caliogna2Micaela Berni3Giulia Gastaldi4Michele Conti5Alice Maria Brancato6Valentina Bina7Domenico Minervini8Stefano Malpede9Anna Chiara Stellato10Francesco Lazzerini11Luca Bruschini12Marco Benazzo13Pietro Canzi14Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Otorhinolaryngology, University of Pavia, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, 27100 Pavia, ItalyDepartment of Civil Engineering and Architecture (DICAr), University of Pavia, Via Ferrata 3, 27100 Pavia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, 27100 Pavia, ItalyDepartment of Otorhinolaryngology, University of Pavia, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyDepartment of Otorhinolaryngology, University of Pavia, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyDepartment of Otorhinolaryngology, University of Pavia, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyOtolaryngology, ENT Audiology and Phoniatrics Unit, University Hospital of Pisa, 56124 Pisa, ItalyOtolaryngology, ENT Audiology and Phoniatrics Unit, University Hospital of Pisa, 56124 Pisa, ItalyDepartment of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, ItalyOssiculoplasty is a surgical operation performed to restore auditory transmission through the reconstruction of the ossicular chain using prosthetics. Tissue bioengineering has assumed a pivotal role in implementing alternatives to conventional ossicular middle ear replacement prostheses, to overcome extrusion while preserving acoustic properties. This <i>in vitro</i> study aims to explore, for the first time in current literature, the feasibility of a biohybrid middle ear prosthesis, composed of titanium surrounded by a bone extracellular matrix as bio-coating. We have hereby studied the adhesion and proliferation of human adipose-derived mesenchymal stem cells (hASC) on titanium scaffolds <i>in vitro</i>. Moreover, we identified the osteogenic differentiation of hASC using an immunofluorescence assay to analyze osteoblasts’ gene expression profiles (Alp, Runx2, Col1a1, Osx, and Bglap), and we counted the presence of collagen as a marker of hASC’s ability to secrete an extracellular matrix. We utilized scanning electron microscopy to evaluate the presence of an extracellular matrix on the scaffolds. Our preliminary data demonstrated the titanium’s ability to support human adipose-derived mesenchymal stem cell colonization, proliferation, and osteoblastic differentiation, in order to obtain a biohybrid device. Our experience seems encouraging; thus, we advocate for further <i>in vivo</i> research to corroborate our results regarding bone transplantation.https://www.mdpi.com/2079-4983/14/12/561middle earossicular replacement prosthesistitaniumbone transplantationtissue engineeringhearing loss |
| spellingShingle | Mario Mosconi Elena Carlotto Laura Caliogna Micaela Berni Giulia Gastaldi Michele Conti Alice Maria Brancato Valentina Bina Domenico Minervini Stefano Malpede Anna Chiara Stellato Francesco Lazzerini Luca Bruschini Marco Benazzo Pietro Canzi Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> Study Journal of Functional Biomaterials middle ear ossicular replacement prosthesis titanium bone transplantation tissue engineering hearing loss |
| title | Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> Study |
| title_full | Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> Study |
| title_fullStr | Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> Study |
| title_full_unstemmed | Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> Study |
| title_short | Titanium Biohybrid Middle Ear Prostheses: A Preliminary <i>In Vitro</i> Study |
| title_sort | titanium biohybrid middle ear prostheses a preliminary i in vitro i study |
| topic | middle ear ossicular replacement prosthesis titanium bone transplantation tissue engineering hearing loss |
| url | https://www.mdpi.com/2079-4983/14/12/561 |
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