New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria
By conducting three different methods, we report on the isolation of five novel strains of non-symbiotic bacteria from crushed infective juveniles (IJs) of four species of entomopathogenic nematodes (EPN) including Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae, and Stei...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2017-12-01
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| Series: | Journal of Crop Protection |
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| Online Access: | http://jcp.modares.ac.ir/article-3-5664-en.html |
| _version_ | 1818211159822565376 |
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| author | Naser Eivazian Kary Davoud Mohammadi Robbie Girling |
| author_facet | Naser Eivazian Kary Davoud Mohammadi Robbie Girling |
| author_sort | Naser Eivazian Kary |
| collection | DOAJ |
| description | By conducting three different methods, we report on the isolation of five novel strains of non-symbiotic bacteria from crushed infective juveniles (IJs) of four species of entomopathogenic nematodes (EPN) including Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae, and Steinernema glaseri and five bacterial species from hemolymph of insect larvae infected with EPNs. Samples of hemolymph of infected Galleria mellonella L. larvae by EPNs and crushed surface sterilized IJs were bulk streaked onto both MacConkey and NBTA agar. To further ensure diagnoses, extracted DNA from IJs bulk was subjected to PCR by 16S-rRNA bacterial universal primers. Bacteria were identified using biochemical and phylogenetic analysis. Based on 16S-rRNA gene sequence, maximum parsimony, maximum likelihood and neighbour joining phylogenetic analyses were conducted, as well as comparisons of predicted RNA secondary structures. Four species of bacteria were identified including: Stenotrophomonas maltophilia strain IR11 from S. feltiae; S. pavanii strain IR20 from S. glaseri; Acinetobacter junii strain IR8 from S. carpocapsae; and Alcaligenes faecalis strains IR1 & IR15 from S. feltiae and H. bacteriophora respectively as non-symbiotic bacteria from IJs and five species probably originated from G. mellonella intestine including Citrobacter gillenii isolate S3, Enterobacter asburiae isolate S4, Klebsiella oxytoca isolate S5, Morganella morganii isolate S6 and Serratia marcescens isolate S6. |
| first_indexed | 2024-12-12T05:28:04Z |
| format | Article |
| id | doaj.art-9e27c4680dba49da80b639a310e1a582 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-12-12T05:28:04Z |
| publishDate | 2017-12-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-9e27c4680dba49da80b639a310e1a5822022-12-22T00:36:25ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2017-12-0164497511New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteriaNaser Eivazian Kary0Davoud Mohammadi1Robbie Girling2 Plant Protection Department, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran. Plant Protection Department, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran. School of Agriculture, Policy and Development, Centre for Agri-Environmental Research, University of Reading, Reading, UK. By conducting three different methods, we report on the isolation of five novel strains of non-symbiotic bacteria from crushed infective juveniles (IJs) of four species of entomopathogenic nematodes (EPN) including Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae, and Steinernema glaseri and five bacterial species from hemolymph of insect larvae infected with EPNs. Samples of hemolymph of infected Galleria mellonella L. larvae by EPNs and crushed surface sterilized IJs were bulk streaked onto both MacConkey and NBTA agar. To further ensure diagnoses, extracted DNA from IJs bulk was subjected to PCR by 16S-rRNA bacterial universal primers. Bacteria were identified using biochemical and phylogenetic analysis. Based on 16S-rRNA gene sequence, maximum parsimony, maximum likelihood and neighbour joining phylogenetic analyses were conducted, as well as comparisons of predicted RNA secondary structures. Four species of bacteria were identified including: Stenotrophomonas maltophilia strain IR11 from S. feltiae; S. pavanii strain IR20 from S. glaseri; Acinetobacter junii strain IR8 from S. carpocapsae; and Alcaligenes faecalis strains IR1 & IR15 from S. feltiae and H. bacteriophora respectively as non-symbiotic bacteria from IJs and five species probably originated from G. mellonella intestine including Citrobacter gillenii isolate S3, Enterobacter asburiae isolate S4, Klebsiella oxytoca isolate S5, Morganella morganii isolate S6 and Serratia marcescens isolate S6.http://jcp.modares.ac.ir/article-3-5664-en.htmlstenotrophomonas maltophilias. pavaniiacinetobacter juniialcaligenes faecaliscitrobacter gilleniienterobacter asburiae klebsiella oxytocamorganella morganiiserratia marcescens |
| spellingShingle | Naser Eivazian Kary Davoud Mohammadi Robbie Girling New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria Journal of Crop Protection stenotrophomonas maltophilia s. pavanii acinetobacter junii alcaligenes faecalis citrobacter gillenii enterobacter asburiae klebsiella oxytoca morganella morganii serratia marcescens |
| title | New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria |
| title_full | New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria |
| title_fullStr | New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria |
| title_full_unstemmed | New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria |
| title_short | New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria |
| title_sort | new reports on dixenic associations between the symbionts of entomopathogenic nematodes photorhabdus and xenorhabdus and non symbiotic bacteria |
| topic | stenotrophomonas maltophilia s. pavanii acinetobacter junii alcaligenes faecalis citrobacter gillenii enterobacter asburiae klebsiella oxytoca morganella morganii serratia marcescens |
| url | http://jcp.modares.ac.ir/article-3-5664-en.html |
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