Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress
Upon exposure to biotic and abiotic stress, plants have developed strategies to adapt to the challenges imposed by these unfavorable conditions. The energetically demanding translation process is one of the main elements regulated to reduce energy consumption and to selectively synthesize proteins i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/7/1160 |
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| author | Zainab Fakih Mélodie B. Plourde Hugo Germain |
| author_facet | Zainab Fakih Mélodie B. Plourde Hugo Germain |
| author_sort | Zainab Fakih |
| collection | DOAJ |
| description | Upon exposure to biotic and abiotic stress, plants have developed strategies to adapt to the challenges imposed by these unfavorable conditions. The energetically demanding translation process is one of the main elements regulated to reduce energy consumption and to selectively synthesize proteins involved in the establishment of an adequate response. Emerging data have shown that ribosomes remodel to adapt to stresses. In <i>Arabidopsis thaliana</i>, ribosomes consist of approximately eighty-one distinct ribosomal proteins (RPs), each of which is encoded by two to seven genes. Recent research has revealed that a mutation in a given single RP in plants can not only affect the functions of the RP itself but can also influence the properties of the ribosome, which could bring about changes in the translation to varying degrees. However, a pending question is whether some RPs enable ribosomes to preferentially translate specific mRNAs. To reveal the role of ribosomal proteins from the small subunit (RPS) in a specific translation, we developed a novel approach to visualize the effect of RPS silencing on the translation of a reporter mRNA (GFP) combined to the 5’UTR of different housekeeping and defense genes. The silencing of genes encoding for <i>NbRPSaA</i>, <i>NbRPS5A</i>, and <i>NbRPS24A</i> in <i>Nicotiana benthamiana</i> decreased the translation of defense genes. The <i>NbRACK1A</i>-silenced plant showed compromised translations of specific antioxidant enzymes. However, the translations of all tested genes were affected in <i>NbRPS27D</i>-silenced plants. These findings suggest that some RPS may be potentially involved in the control of protein translation. |
| first_indexed | 2024-03-11T01:15:49Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T01:15:49Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9e286e03e8f14c439ce037ec4a1574d82023-11-18T18:32:17ZengMDPI AGBiomolecules2218-273X2023-07-01137116010.3390/biom13071160Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under StressZainab Fakih0Mélodie B. Plourde1Hugo Germain2Department of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières, Trois-Rivières, QC G9A 5H9, CanadaDepartment of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières, Trois-Rivières, QC G9A 5H9, CanadaDepartment of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières, Trois-Rivières, QC G9A 5H9, CanadaUpon exposure to biotic and abiotic stress, plants have developed strategies to adapt to the challenges imposed by these unfavorable conditions. The energetically demanding translation process is one of the main elements regulated to reduce energy consumption and to selectively synthesize proteins involved in the establishment of an adequate response. Emerging data have shown that ribosomes remodel to adapt to stresses. In <i>Arabidopsis thaliana</i>, ribosomes consist of approximately eighty-one distinct ribosomal proteins (RPs), each of which is encoded by two to seven genes. Recent research has revealed that a mutation in a given single RP in plants can not only affect the functions of the RP itself but can also influence the properties of the ribosome, which could bring about changes in the translation to varying degrees. However, a pending question is whether some RPs enable ribosomes to preferentially translate specific mRNAs. To reveal the role of ribosomal proteins from the small subunit (RPS) in a specific translation, we developed a novel approach to visualize the effect of RPS silencing on the translation of a reporter mRNA (GFP) combined to the 5’UTR of different housekeeping and defense genes. The silencing of genes encoding for <i>NbRPSaA</i>, <i>NbRPS5A</i>, and <i>NbRPS24A</i> in <i>Nicotiana benthamiana</i> decreased the translation of defense genes. The <i>NbRACK1A</i>-silenced plant showed compromised translations of specific antioxidant enzymes. However, the translations of all tested genes were affected in <i>NbRPS27D</i>-silenced plants. These findings suggest that some RPS may be potentially involved in the control of protein translation.https://www.mdpi.com/2218-273X/13/7/1160<i>Nicotiana benthamiana</i><i>Arabidopsis thaliana</i>translation regulationribosomal proteins from the small subunit (RPS)VIGS5′untranslated regions |
| spellingShingle | Zainab Fakih Mélodie B. Plourde Hugo Germain Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress Biomolecules <i>Nicotiana benthamiana</i> <i>Arabidopsis thaliana</i> translation regulation ribosomal proteins from the small subunit (RPS) VIGS 5′untranslated regions |
| title | Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress |
| title_full | Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress |
| title_fullStr | Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress |
| title_full_unstemmed | Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress |
| title_short | Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress |
| title_sort | differential participation of plant ribosomal proteins from the small ribosomal subunit in protein translation under stress |
| topic | <i>Nicotiana benthamiana</i> <i>Arabidopsis thaliana</i> translation regulation ribosomal proteins from the small subunit (RPS) VIGS 5′untranslated regions |
| url | https://www.mdpi.com/2218-273X/13/7/1160 |
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