Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model
We evaluated the synergistic effects of pentosan polysulfate sodium (PPS) and mesenchymal stem cells (MSCs) in an interstitial cystitis (IC) rat model. After generation of the IC rat model, the rats were divided into 4 groups according to the treatment they received: phosphate-buffered saline inject...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Stem Cell Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120301045 |
| _version_ | 1818159077975392256 |
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| author | Bum Soo Kim So Young Chun Eun Hye Lee Jae-Wook Chung Jun Nyung Lee Yun-Sok Ha Jae Young Choi Phil Hyun Song Tae Gyun Kwon Man-Hoon Han Dae Hwan Kim Eun Sang Yoo |
| author_facet | Bum Soo Kim So Young Chun Eun Hye Lee Jae-Wook Chung Jun Nyung Lee Yun-Sok Ha Jae Young Choi Phil Hyun Song Tae Gyun Kwon Man-Hoon Han Dae Hwan Kim Eun Sang Yoo |
| author_sort | Bum Soo Kim |
| collection | DOAJ |
| description | We evaluated the synergistic effects of pentosan polysulfate sodium (PPS) and mesenchymal stem cells (MSCs) in an interstitial cystitis (IC) rat model. After generation of the IC rat model, the rats were divided into 4 groups according to the treatment they received: phosphate-buffered saline injection into bladder submucosa, daily oral PPS feeding, MSC injection into bladder submucosa, or MSC injection into bladder submucosa with daily oral PPS feeding. After treatment, conscious cystometry and pain scale measurement were performed and their bladders were obtained for histological and proinflammatory-related gene expression analysis. On cystometric analysis, all treatment groups showed significantly increased intercontraction intervals and lower pain scores compared to those of the control group. Histological analysis revealed regenerated urothelium, less fibrosis, and decreased mast cell infiltration in all treatment groups compared to the control group. Significantly lower expression of TNF-α, IFN-γ, MCP, IL-6, TLR2, and TLR11 was observed in the PPS with MSC group compared to the other groups. Combination therapy with PPS and MSCs showed histological and functional effects in an IC rat model, including synergistic effects leading to increased intercontraction interval and decreased inflammatory reactions. |
| first_indexed | 2024-12-11T15:40:15Z |
| format | Article |
| id | doaj.art-9e29dc32454b4f09af0d056deeaa4f9a |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-11T15:40:15Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-9e29dc32454b4f09af0d056deeaa4f9a2022-12-22T00:59:50ZengElsevierStem Cell Research1873-50612020-05-0145Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat modelBum Soo Kim0So Young Chun1Eun Hye Lee2Jae-Wook Chung3Jun Nyung Lee4Yun-Sok Ha5Jae Young Choi6Phil Hyun Song7Tae Gyun Kwon8Man-Hoon Han9Dae Hwan Kim10Eun Sang Yoo11Department of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, South KoreaBioMedical Research Institute, Kyungpook National University Hospital, Daegu, South KoreaBioMedical Research Institute, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South KoreaDepartment of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South KoreaDepartment of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South KoreaDepartment of Urology, Yeungnam University College of Medicine, Daegu, South KoreaDepartment of Urology, Yeungnam University College of Medicine, Daegu, South KoreaDepartment of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South KoreaDepartment of Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Laboratory Animal Research Support Team, Yeungnam University Hospital, Daegu, South KoreaDepartment of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, South Korea; Corresponding author. Address: Department of Urology, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu, Korea. Postal code: 41944. Telephone: 82 53 2005851; Fax: 82-53-421-9618.We evaluated the synergistic effects of pentosan polysulfate sodium (PPS) and mesenchymal stem cells (MSCs) in an interstitial cystitis (IC) rat model. After generation of the IC rat model, the rats were divided into 4 groups according to the treatment they received: phosphate-buffered saline injection into bladder submucosa, daily oral PPS feeding, MSC injection into bladder submucosa, or MSC injection into bladder submucosa with daily oral PPS feeding. After treatment, conscious cystometry and pain scale measurement were performed and their bladders were obtained for histological and proinflammatory-related gene expression analysis. On cystometric analysis, all treatment groups showed significantly increased intercontraction intervals and lower pain scores compared to those of the control group. Histological analysis revealed regenerated urothelium, less fibrosis, and decreased mast cell infiltration in all treatment groups compared to the control group. Significantly lower expression of TNF-α, IFN-γ, MCP, IL-6, TLR2, and TLR11 was observed in the PPS with MSC group compared to the other groups. Combination therapy with PPS and MSCs showed histological and functional effects in an IC rat model, including synergistic effects leading to increased intercontraction interval and decreased inflammatory reactions.http://www.sciencedirect.com/science/article/pii/S1873506120301045Interstitial cystitisRatsStem cellsPentosan polysulfate sodium |
| spellingShingle | Bum Soo Kim So Young Chun Eun Hye Lee Jae-Wook Chung Jun Nyung Lee Yun-Sok Ha Jae Young Choi Phil Hyun Song Tae Gyun Kwon Man-Hoon Han Dae Hwan Kim Eun Sang Yoo Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model Stem Cell Research Interstitial cystitis Rats Stem cells Pentosan polysulfate sodium |
| title | Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model |
| title_full | Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model |
| title_fullStr | Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model |
| title_full_unstemmed | Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model |
| title_short | Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model |
| title_sort | efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue derived stem cells for the management of interstitial cystitis in a rat model |
| topic | Interstitial cystitis Rats Stem cells Pentosan polysulfate sodium |
| url | http://www.sciencedirect.com/science/article/pii/S1873506120301045 |
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