Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

Abstract The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR c...

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Bibliographic Details
Main Authors: Liting Cao, Yancheng Zhou, Lin Huang, Shiqi Dong, Yue Ma
Format: Article
Language:English
Published: SpringerOpen 2017-05-01
Series:AMB Express
Subjects:
PCR cloning
Prokaryotic expression
Eukaryotic expression
Lysis gene E
Positive selection
Online Access:http://link.springer.com/article/10.1186/s13568-017-0386-1

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