Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM
Abstract The present study aimed to explore the three-dimensional (3D) ultrastructure of interstitial cells (ICs) within the lamina propria of the murine vas deferens and the spatial relationships between epithelial cells and surrounding cells. Focused ion beam scanning electron microscopy and confo...
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| Format: | Article |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13245-7 |
| _version_ | 1811258060466814976 |
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| author | Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Akinobu Togo Keisuke Ohta Kei-Ichiro Nakamura Tsukasa Igawa |
| author_facet | Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Akinobu Togo Keisuke Ohta Kei-Ichiro Nakamura Tsukasa Igawa |
| author_sort | Tasuku Hiroshige |
| collection | DOAJ |
| description | Abstract The present study aimed to explore the three-dimensional (3D) ultrastructure of interstitial cells (ICs) within the lamina propria of the murine vas deferens and the spatial relationships between epithelial cells and surrounding cells. Focused ion beam scanning electron microscopy and confocal laser scanning microscopy were performed. ICs within the lamina propria had a flat, sheet-like structure of cytoplasm with multiple cellular processes. In addition, two types of 3D structures that comprised cell processes of flat, sheet-like ICs were observed: one was an accordion fold-like structure and the other was a rod-shaped structure. ICs were located parallel to the epithelium and were connected to each other via gap junctions or adherens junctions. Moreover, multiple sphere-shaped extracellular vesicle-like structures were frequently observed around the ICs. The ICs formed a complex 3D network comprising sheet-like cytoplasm and multiple cell processes with different 3D structures. From this morphological study, we noted that ICs within the lamina propria of murine vas deferens may be involved in signal transmission between the epithelium and smooth muscle cells by physical interaction and by exchanging extracellular vesicles. |
| first_indexed | 2024-04-12T18:07:28Z |
| format | Article |
| id | doaj.art-9e2eca84c3964ce8b935f29bc869c649 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T18:07:28Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9e2eca84c3964ce8b935f29bc869c6492022-12-22T03:21:56ZengNature PortfolioScientific Reports2045-23222022-06-0112111110.1038/s41598-022-13245-7Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEMTasuku Hiroshige0Kei-Ichiro Uemura1Shingo Hirashima2Akinobu Togo3Keisuke Ohta4Kei-Ichiro Nakamura5Tsukasa Igawa6Department of Urology, Kurume University School of MedicineDepartment of Urology, Kurume University School of MedicineDivision Microscopic and Development Anatomy, Department of Anatomy, Kurume University School of MedicineAdvanced Imaging Research Center, Kurume University School of MedicineDivision Microscopic and Development Anatomy, Department of Anatomy, Kurume University School of MedicineCognitive and Molecular Research Institute of Brain Diseases, Kurume University School of MedicineDepartment of Urology, Kurume University School of MedicineAbstract The present study aimed to explore the three-dimensional (3D) ultrastructure of interstitial cells (ICs) within the lamina propria of the murine vas deferens and the spatial relationships between epithelial cells and surrounding cells. Focused ion beam scanning electron microscopy and confocal laser scanning microscopy were performed. ICs within the lamina propria had a flat, sheet-like structure of cytoplasm with multiple cellular processes. In addition, two types of 3D structures that comprised cell processes of flat, sheet-like ICs were observed: one was an accordion fold-like structure and the other was a rod-shaped structure. ICs were located parallel to the epithelium and were connected to each other via gap junctions or adherens junctions. Moreover, multiple sphere-shaped extracellular vesicle-like structures were frequently observed around the ICs. The ICs formed a complex 3D network comprising sheet-like cytoplasm and multiple cell processes with different 3D structures. From this morphological study, we noted that ICs within the lamina propria of murine vas deferens may be involved in signal transmission between the epithelium and smooth muscle cells by physical interaction and by exchanging extracellular vesicles.https://doi.org/10.1038/s41598-022-13245-7 |
| spellingShingle | Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Akinobu Togo Keisuke Ohta Kei-Ichiro Nakamura Tsukasa Igawa Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM Scientific Reports |
| title | Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM |
| title_full | Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM |
| title_fullStr | Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM |
| title_full_unstemmed | Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM |
| title_short | Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM |
| title_sort | three dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and fib sem |
| url | https://doi.org/10.1038/s41598-022-13245-7 |
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