Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validation
Sanggua Drink (SGD) is an experienced formula for clinical treatment of type 2 diabetes mellitus (T2DM). Network pharmacology and experiments were combined to explore the potential mechanism of action of SGD on T2DM. The material basis and action mechanism of SGD were investigated to reveal the acti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023052349 |
| _version_ | 1827892290560983040 |
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| author | Yu Cai Simin Liu Fei Zeng Zhiwei Rao Chunchao Yan Qichang Xing Yunzhong Chen |
| author_facet | Yu Cai Simin Liu Fei Zeng Zhiwei Rao Chunchao Yan Qichang Xing Yunzhong Chen |
| author_sort | Yu Cai |
| collection | DOAJ |
| description | Sanggua Drink (SGD) is an experienced formula for clinical treatment of type 2 diabetes mellitus (T2DM). Network pharmacology and experiments were combined to explore the potential mechanism of action of SGD on T2DM. The material basis and action mechanism of SGD were investigated to reveal the active components of SGD, potential target prediction was conducted from TargetNet, PharmMapper; Cytoscape was used to construct PPI network and component-target-pathway (C-T-P) network diagram to interpret biological processes and enrich action pathways. 54 compounds and 41 key target proteins were screened, and a total of 98 signaling pathways were obtained. In vivo experiments, the levels of p-AMPK (P < 0.01), p-ACC and p-AKT were significantly increased in the mice with SGD intervention compared to the db/db mice, while level of FOXO1 were decreased. The results suggested that SGD might improve insulin resistance and glucose metabolism in T2DM mice by activating the AMPK/Akt signaling pathway. |
| first_indexed | 2024-03-12T21:37:40Z |
| format | Article |
| id | doaj.art-9e306e76309a4277b98b1ecade6d474c |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:37:40Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-9e306e76309a4277b98b1ecade6d474c2023-07-27T05:58:17ZengElsevierHeliyon2405-84402023-07-0197e18026Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validationYu Cai0Simin Liu1Fei Zeng2Zhiwei Rao3Chunchao Yan4Qichang Xing5Yunzhong Chen6Hubei Provincial Research Center for TCM Health Food Engineering, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, ChinaHubei Provincial Research Center for TCM Health Food Engineering, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, ChinaHubei Provincial Research Center for TCM Health Food Engineering, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, ChinaCentral Hospital of Xianning, The First Affiliate Hospital of Hubei University of Science, ChinaHubei Provincial Research Center for TCM Health Food Engineering, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, ChinaHubei Provincial Research Center for TCM Health Food Engineering, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, ChinaHubei Provincial Research Center for TCM Health Food Engineering, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China; Corresponding author.Sanggua Drink (SGD) is an experienced formula for clinical treatment of type 2 diabetes mellitus (T2DM). Network pharmacology and experiments were combined to explore the potential mechanism of action of SGD on T2DM. The material basis and action mechanism of SGD were investigated to reveal the active components of SGD, potential target prediction was conducted from TargetNet, PharmMapper; Cytoscape was used to construct PPI network and component-target-pathway (C-T-P) network diagram to interpret biological processes and enrich action pathways. 54 compounds and 41 key target proteins were screened, and a total of 98 signaling pathways were obtained. In vivo experiments, the levels of p-AMPK (P < 0.01), p-ACC and p-AKT were significantly increased in the mice with SGD intervention compared to the db/db mice, while level of FOXO1 were decreased. The results suggested that SGD might improve insulin resistance and glucose metabolism in T2DM mice by activating the AMPK/Akt signaling pathway.http://www.sciencedirect.com/science/article/pii/S2405844023052349Sanggua drinkT2DMAMPK/Aktdb/db miceInsulin resistance |
| spellingShingle | Yu Cai Simin Liu Fei Zeng Zhiwei Rao Chunchao Yan Qichang Xing Yunzhong Chen Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validation Heliyon Sanggua drink T2DM AMPK/Akt db/db mice Insulin resistance |
| title | Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validation |
| title_full | Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validation |
| title_fullStr | Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validation |
| title_full_unstemmed | Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validation |
| title_short | Exploring the protective effect of Sangggua Drink against type 2 diabetes mellitus in db/db mice using a network pharmacological approach and experimental validation |
| title_sort | exploring the protective effect of sangggua drink against type 2 diabetes mellitus in db db mice using a network pharmacological approach and experimental validation |
| topic | Sanggua drink T2DM AMPK/Akt db/db mice Insulin resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023052349 |
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