Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits
Objective To investigate whether ischemic preconditioning (IP) attenuates endothelial injury induced by arterial puncture. Methods Rabbits were divided into control group, ischemic preconditioning group (IP group), femoral artery puncture group (P group) and ischemic preconditioning+femoral artery...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-12-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-12-1817.pdf |
| _version_ | 1797365872135766016 |
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| author | LIU Peng, LI Kai-yuan, YANG Yang, MAN Yi-long, DU Feng-li, LIU Miao |
| author_facet | LIU Peng, LI Kai-yuan, YANG Yang, MAN Yi-long, DU Feng-li, LIU Miao |
| author_sort | LIU Peng, LI Kai-yuan, YANG Yang, MAN Yi-long, DU Feng-li, LIU Miao |
| collection | DOAJ |
| description | Objective To investigate whether ischemic preconditioning (IP) attenuates endothelial injury induced by arterial puncture. Methods Rabbits were divided into control group, ischemic preconditioning group (IP group), femoral artery puncture group (P group) and ischemic preconditioning+femoral artery puncture group (IP-P group). The expression of PI3K, AKT and eNOS in endothelial tissue was determined by immuno-histochemistry. p-AKT and p-eNOS were detected by Western blot. eNOS was detected by RT-qPCR. Plasma NO was detected by enzyme-linked immunosorbent assay(ELISA). Results The expression of PI3K, AKT and eNOS in the vascular endothelium of IP-P group was higher than that from P group and lower than that from IP group(P＜0.05). The expression of p-AKT and p-eNOS in IP-P group was higher than that from P group(P＜0.05). The expression of eNOS in IP-P group was higher than that from P group and lower than that from IP group(P＜0.05). Plasma NO level in IP-P group was higher than that in P group and lower than that in IP group (P＜0.05). Conclusions IP promotes eNOS phos-phorylation and NO synthesis in rabbit vascular endothelium, which may be one of the potential mechanisms by which IP reduces endothelial injury before arterial puncture. |
| first_indexed | 2024-03-08T16:55:08Z |
| format | Article |
| id | doaj.art-9e306f7200be4d9f9c6a2b35d829527a |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:08Z |
| publishDate | 2022-12-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-9e306f7200be4d9f9c6a2b35d829527a2024-01-05T02:36:47ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-12-0142121817182210.16352/j.issn.1001-6325.2022.12.1817Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbitsLIU Peng, LI Kai-yuan, YANG Yang, MAN Yi-long, DU Feng-li, LIU Miao01. Department of Cardiovascular Medicine, Affiliated Central Hospital of Shandong First Medical University, Jinan 250000; ;2. Shandong Clinical Center of Public Health, Jinan 250000, ChinaObjective To investigate whether ischemic preconditioning (IP) attenuates endothelial injury induced by arterial puncture. Methods Rabbits were divided into control group, ischemic preconditioning group (IP group), femoral artery puncture group (P group) and ischemic preconditioning+femoral artery puncture group (IP-P group). The expression of PI3K, AKT and eNOS in endothelial tissue was determined by immuno-histochemistry. p-AKT and p-eNOS were detected by Western blot. eNOS was detected by RT-qPCR. Plasma NO was detected by enzyme-linked immunosorbent assay(ELISA). Results The expression of PI3K, AKT and eNOS in the vascular endothelium of IP-P group was higher than that from P group and lower than that from IP group(P＜0.05). The expression of p-AKT and p-eNOS in IP-P group was higher than that from P group(P＜0.05). The expression of eNOS in IP-P group was higher than that from P group and lower than that from IP group(P＜0.05). Plasma NO level in IP-P group was higher than that in P group and lower than that in IP group (P＜0.05). Conclusions IP promotes eNOS phos-phorylation and NO synthesis in rabbit vascular endothelium, which may be one of the potential mechanisms by which IP reduces endothelial injury before arterial puncture.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-12-1817.pdfischemic preconditioning|femoral artery puncture|pi3k/akt|enos |
| spellingShingle | LIU Peng, LI Kai-yuan, YANG Yang, MAN Yi-long, DU Feng-li, LIU Miao Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits Jichu yixue yu linchuang ischemic preconditioning|femoral artery puncture|pi3k/akt|enos |
| title | Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits |
| title_full | Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits |
| title_fullStr | Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits |
| title_full_unstemmed | Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits |
| title_short | Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits |
| title_sort | ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits |
| topic | ischemic preconditioning|femoral artery puncture|pi3k/akt|enos |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-12-1817.pdf |
| work_keys_str_mv | AT liupenglikaiyuanyangyangmanyilongdufengliliumiao ischemicpreconditioningattenuatesendothelialinjuryinducedbyfemoralarterypunctureinrabbits |