Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants
Abstract In vitro culture techniques are recognized as efficient strategies for large-scale plant production, as well as providing alternatives for plant conservation. In this study the micropropagation of Tarenaya rosea was established using petiole and foliar blade segments cultivated on MS medium...
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| Format: | Article |
| Language: | English |
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Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
2021-03-01
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| Series: | Rodriguésia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100211&tlng=en |
| _version_ | 1828132450409119744 |
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| author | Claudia Simões-Gurgel Tatiana Carvalho de Castro Cátia Henriques Callado Lívia da Silva Cordeiro Norma Albarello |
| author_facet | Claudia Simões-Gurgel Tatiana Carvalho de Castro Cátia Henriques Callado Lívia da Silva Cordeiro Norma Albarello |
| author_sort | Claudia Simões-Gurgel |
| collection | DOAJ |
| description | Abstract In vitro culture techniques are recognized as efficient strategies for large-scale plant production, as well as providing alternatives for plant conservation. In this study the micropropagation of Tarenaya rosea was established using petiole and foliar blade segments cultivated on MS medium with 6-benzyladenine (BA) and/or 6-furfurylaminopurine (KIN). The regeneration rate from explants was evaluated after 30-days in culture, as well as the proliferation rate from explant-derived shoots, reached after four subcultures performed at 30-days in culture. In vitro propagation occurred by both direct (DO) and indirect (IO) organogenesis. The highest regeneration rates by DO (50% to 100%) were reached on media containing only BA, while morphogenic calluses (IO) were mainly formed with BA+KIN. Explants on media with BA showed the presence of small black nodules on their surface, and histological analysis revealed the presence of trichomes with anthocyanin content. Elongation and rooting were reached on growth regulator-free MS. Acclimatization rates around 80% were achieved and the in vitro-regenerated plants were successfully maintained under field conditions. Results show significant morphogenetic potential of T. rosea from leaf explants, mainly when cultivated in the presence of 4.4 µM BA, providing a new alternative source of plant material for biotechnological and in vitro conservation studies. |
| first_indexed | 2024-04-11T17:03:47Z |
| format | Article |
| id | doaj.art-9e30dcd238fd4a7aa4081b50f45211ec |
| institution | Directory Open Access Journal |
| issn | 2175-7860 |
| language | English |
| last_indexed | 2024-04-11T17:03:47Z |
| publishDate | 2021-03-01 |
| publisher | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| record_format | Article |
| series | Rodriguésia |
| spelling | doaj.art-9e30dcd238fd4a7aa4081b50f45211ec2022-12-22T04:13:06ZengInstituto de Pesquisas Jardim Botânico do Rio de JaneiroRodriguésia2175-78602021-03-017210.1590/2175-7860202172020Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explantsClaudia Simões-Gurgelhttps://orcid.org/0000-0001-8358-2568Tatiana Carvalho de Castrohttps://orcid.org/0000-0003-0554-6873Cátia Henriques Calladohttps://orcid.org/0000-0003-2190-3375Lívia da Silva Cordeirohttps://orcid.org/0000-0002-6964-7000Norma Albarellohttps://orcid.org/0000-0001-5803-2070Abstract In vitro culture techniques are recognized as efficient strategies for large-scale plant production, as well as providing alternatives for plant conservation. In this study the micropropagation of Tarenaya rosea was established using petiole and foliar blade segments cultivated on MS medium with 6-benzyladenine (BA) and/or 6-furfurylaminopurine (KIN). The regeneration rate from explants was evaluated after 30-days in culture, as well as the proliferation rate from explant-derived shoots, reached after four subcultures performed at 30-days in culture. In vitro propagation occurred by both direct (DO) and indirect (IO) organogenesis. The highest regeneration rates by DO (50% to 100%) were reached on media containing only BA, while morphogenic calluses (IO) were mainly formed with BA+KIN. Explants on media with BA showed the presence of small black nodules on their surface, and histological analysis revealed the presence of trichomes with anthocyanin content. Elongation and rooting were reached on growth regulator-free MS. Acclimatization rates around 80% were achieved and the in vitro-regenerated plants were successfully maintained under field conditions. Results show significant morphogenetic potential of T. rosea from leaf explants, mainly when cultivated in the presence of 4.4 µM BA, providing a new alternative source of plant material for biotechnological and in vitro conservation studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100211&tlng=enanthocyaninCleome roseacytokininsorganogenesis |
| spellingShingle | Claudia Simões-Gurgel Tatiana Carvalho de Castro Cátia Henriques Callado Lívia da Silva Cordeiro Norma Albarello Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants Rodriguésia anthocyanin Cleome rosea cytokinins organogenesis |
| title | Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants |
| title_full | Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants |
| title_fullStr | Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants |
| title_full_unstemmed | Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants |
| title_short | Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants |
| title_sort | micropropagation of tarenaya rosea cleomaceae from leaf explants |
| topic | anthocyanin Cleome rosea cytokinins organogenesis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100211&tlng=en |
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