Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway
Gold nanoparticles (AuNPs) is the most excellent anticancer theranostic nanoparticles synthesized through efficient, simple and green synthesis method using extracts of Trichosanthes kirilowii, extensively characterized by UV-spectroscopy, FT-IR and TEM techniques. The AuNPs, synthesized by means of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-12-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1626412 |
| _version_ | 1811291882962026496 |
|---|---|
| author | Xiaodong Han Xiaojia Jiang Lanjie Guo Yongxin Wang Vishnu Priya Veeraraghavan Surapaneni Krishna Mohan Zhigang Wang Dandan Cao |
| author_facet | Xiaodong Han Xiaojia Jiang Lanjie Guo Yongxin Wang Vishnu Priya Veeraraghavan Surapaneni Krishna Mohan Zhigang Wang Dandan Cao |
| author_sort | Xiaodong Han |
| collection | DOAJ |
| description | Gold nanoparticles (AuNPs) is the most excellent anticancer theranostic nanoparticles synthesized through efficient, simple and green synthesis method using extracts of Trichosanthes kirilowii, extensively characterized by UV-spectroscopy, FT-IR and TEM techniques. The AuNPs, synthesized by means of T. kirilowii extracts identified that nanoparticles were ∼50 nm in size, which is an admirable nano dimension attained by green synthesis. In agreement with the outcome of microscopic cellular morphological observations, MTT assay showed effective, selective, anticarcinogenic effect of AuNPs on HCT-116 cells in a dose-dependent manner. The AuNPs significantly enhance ROS generation, cause mitochondrial membrane damage and induce morphological changes using AO/EtBr staining assay. Furthermore, AuNPs treatment induces G0/G1 phase cell-cycle arrest in HCT-116 cells. Also, AuNPs treatment activates caspase expression and downregulates the anti-apoptotic expression in HCT-116 cells. Our results point out that the phytoconsituents isolated from T. kirilowii can act as appropriate reducing and stabilizing agents in the properties of AuNPs; hereby, it leads to the green synthesis of an anti-carcinogenic agent with highly efficient potential for cancer treatment. |
| first_indexed | 2024-04-13T04:37:02Z |
| format | Article |
| id | doaj.art-9e313bb1f5584197a60e4ff581fd14db |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T04:37:02Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-9e313bb1f5584197a60e4ff581fd14db2022-12-22T03:02:08ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014713577358410.1080/21691401.2019.1626412Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathwayXiaodong Han0Xiaojia Jiang1Lanjie Guo2Yongxin Wang3Vishnu Priya Veeraraghavan4Surapaneni Krishna Mohan5Zhigang Wang6Dandan Cao7Department of Gastroenterology, Cangzhou People's Hospital, Cangzhou, Hebei, ChinaDepartment of Gastroenterology, Cangzhou People's Hospital, Cangzhou, Hebei, ChinaDepartment of Gastroenterology, Cangzhou People's Hospital, Cangzhou, Hebei, ChinaDepartment of Gastroenterology, Cangzhou People's Hospital, Cangzhou, Hebei, ChinaDepartment of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences,Saveetha University, Chennai, IndiaDepartment of Medical Biochemistry,College of Applied Medical Sciences – Jubail (CAMSJ), Imam Abdulrahman Bin Faisal University, Jubail Industrial City, Al Jubail, Kingdom of Saudi Arabia (KSA)Department of Gastroenterology, Cangzhou People's Hospital, Cangzhou, Hebei, ChinaDepartment of Nephrology, Cangzhou People's Hospital, Cangzhou, Hebei, ChinaGold nanoparticles (AuNPs) is the most excellent anticancer theranostic nanoparticles synthesized through efficient, simple and green synthesis method using extracts of Trichosanthes kirilowii, extensively characterized by UV-spectroscopy, FT-IR and TEM techniques. The AuNPs, synthesized by means of T. kirilowii extracts identified that nanoparticles were ∼50 nm in size, which is an admirable nano dimension attained by green synthesis. In agreement with the outcome of microscopic cellular morphological observations, MTT assay showed effective, selective, anticarcinogenic effect of AuNPs on HCT-116 cells in a dose-dependent manner. The AuNPs significantly enhance ROS generation, cause mitochondrial membrane damage and induce morphological changes using AO/EtBr staining assay. Furthermore, AuNPs treatment induces G0/G1 phase cell-cycle arrest in HCT-116 cells. Also, AuNPs treatment activates caspase expression and downregulates the anti-apoptotic expression in HCT-116 cells. Our results point out that the phytoconsituents isolated from T. kirilowii can act as appropriate reducing and stabilizing agents in the properties of AuNPs; hereby, it leads to the green synthesis of an anti-carcinogenic agent with highly efficient potential for cancer treatment.https://www.tandfonline.com/doi/10.1080/21691401.2019.1626412AuNPsHCT-116cell-cycle arrestapoptosisROSMMP |
| spellingShingle | Xiaodong Han Xiaojia Jiang Lanjie Guo Yongxin Wang Vishnu Priya Veeraraghavan Surapaneni Krishna Mohan Zhigang Wang Dandan Cao Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway Artificial Cells, Nanomedicine, and Biotechnology AuNPs HCT-116 cell-cycle arrest apoptosis ROS MMP |
| title | Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway |
| title_full | Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway |
| title_fullStr | Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway |
| title_full_unstemmed | Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway |
| title_short | Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway |
| title_sort | anticarcinogenic potential of gold nanoparticles synthesized from trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway |
| topic | AuNPs HCT-116 cell-cycle arrest apoptosis ROS MMP |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1626412 |
| work_keys_str_mv | AT xiaodonghan anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway AT xiaojiajiang anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway AT lanjieguo anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway AT yongxinwang anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway AT vishnupriyaveeraraghavan anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway AT surapanenikrishnamohan anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway AT zhigangwang anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway AT dandancao anticarcinogenicpotentialofgoldnanoparticlessynthesizedfromtrichosantheskirilowiiincoloncancercellsthroughtheinductionofapoptoticpathway |