Elastic constant of Dendrobium protoplasts in AC electric fields
This work reports elongation of Dendrobium protoplasts in an ac electric field between two cylindrical electrodes. A protoplast firstly was translated towards an electrode by dielectrophoretic force in 17 kV.m-1 field strength at 1 MHz, and secondly it was elongated due to an interaction between an...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2002-11-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/24_Suppl_membrane/05Elastic.pdf |
| _version_ | 1811342321046781952 |
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| author | Pikul Wanichapichart Kanokkarn Maswiwat Kamnoon Kanchanapoom |
| author_facet | Pikul Wanichapichart Kanokkarn Maswiwat Kamnoon Kanchanapoom |
| author_sort | Pikul Wanichapichart |
| collection | DOAJ |
| description | This work reports elongation of Dendrobium protoplasts in an ac electric field between two cylindrical electrodes. A protoplast firstly was translated towards an electrode by dielectrophoretic force in 17 kV.m-1 field strength at 1 MHz, and secondly it was elongated due to an interaction between an induced electric dipole (μ) and the electric field (E). Protoplast elongation was observed by varying both the field strength at 30, 45, 60, and 85 kV.m-1 and field frequency at 0.5, 1, 5, and 10 MHz. For a given field frequency and field strength, a parameter a/b (major/minor axis) was measured as the protoplast elongation.Two-step elongation and restoration phases were observed. The former was completed within 2 minutes of field exposure, and the latter was completed within 15 seconds regardless of the field exposure time between 3 and 20 minutes. The evidence of a complete restoration indicated that the elasticity of the protoplast membrane obeyed Hooke’s law. This study also found that elastic constant k of the membrane varied non-linearly with the field strength. It was found to be from 0.04 to 0.08 mN.m-1, dependent on the field frequency. |
| first_indexed | 2024-04-13T19:09:30Z |
| format | Article |
| id | doaj.art-9e31fcb88c5e40b79e5e179ed25ec8d1 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-13T19:09:30Z |
| publishDate | 2002-11-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-9e31fcb88c5e40b79e5e179ed25ec8d12022-12-22T02:33:53ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952002-11-0124Suppl.799806Elastic constant of Dendrobium protoplasts in AC electric fieldsPikul WanichapichartKanokkarn MaswiwatKamnoon KanchanapoomThis work reports elongation of Dendrobium protoplasts in an ac electric field between two cylindrical electrodes. A protoplast firstly was translated towards an electrode by dielectrophoretic force in 17 kV.m-1 field strength at 1 MHz, and secondly it was elongated due to an interaction between an induced electric dipole (μ) and the electric field (E). Protoplast elongation was observed by varying both the field strength at 30, 45, 60, and 85 kV.m-1 and field frequency at 0.5, 1, 5, and 10 MHz. For a given field frequency and field strength, a parameter a/b (major/minor axis) was measured as the protoplast elongation.Two-step elongation and restoration phases were observed. The former was completed within 2 minutes of field exposure, and the latter was completed within 15 seconds regardless of the field exposure time between 3 and 20 minutes. The evidence of a complete restoration indicated that the elasticity of the protoplast membrane obeyed Hooke’s law. This study also found that elastic constant k of the membrane varied non-linearly with the field strength. It was found to be from 0.04 to 0.08 mN.m-1, dependent on the field frequency.http://www.sjst.psu.ac.th/journal/24_Suppl_membrane/05Elastic.pdfdielectrophoresiselastic constantelongationalternating electric fieldprotoplasts |
| spellingShingle | Pikul Wanichapichart Kanokkarn Maswiwat Kamnoon Kanchanapoom Elastic constant of Dendrobium protoplasts in AC electric fields Songklanakarin Journal of Science and Technology (SJST) dielectrophoresis elastic constant elongation alternating electric field protoplasts |
| title | Elastic constant of Dendrobium protoplasts in AC electric fields |
| title_full | Elastic constant of Dendrobium protoplasts in AC electric fields |
| title_fullStr | Elastic constant of Dendrobium protoplasts in AC electric fields |
| title_full_unstemmed | Elastic constant of Dendrobium protoplasts in AC electric fields |
| title_short | Elastic constant of Dendrobium protoplasts in AC electric fields |
| title_sort | elastic constant of dendrobium protoplasts in ac electric fields |
| topic | dielectrophoresis elastic constant elongation alternating electric field protoplasts |
| url | http://www.sjst.psu.ac.th/journal/24_Suppl_membrane/05Elastic.pdf |
| work_keys_str_mv | AT pikulwanichapichart elasticconstantofdendrobiumprotoplastsinacelectricfields AT kanokkarnmaswiwat elasticconstantofdendrobiumprotoplastsinacelectricfields AT kamnoonkanchanapoom elasticconstantofdendrobiumprotoplastsinacelectricfields |