Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases
Background: The existing data show that inflammasomes play a role in periodontal disease pathogenesis. However, their role in the pathogenesis of periodontitis and coronary heart disease remains unclear. This study had the objective of assessing NLRP3 (rs4612666) and CARD8 (rs2043211) gene polymorph...
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MDPI AG
2021-06-01
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| author | Jaideep Mahendra Abirami Nayaki Rao Little Mahendra Hytham N. Fageeh Hammam Ibrahim Fageeh Thodur Madapusi Balaji Saranya Varadarajan Raghunathan Jagannathan Deepak Mehta Venkata V. Suresh A. Thirumal Raj Shankargouda Patil |
| author_facet | Jaideep Mahendra Abirami Nayaki Rao Little Mahendra Hytham N. Fageeh Hammam Ibrahim Fageeh Thodur Madapusi Balaji Saranya Varadarajan Raghunathan Jagannathan Deepak Mehta Venkata V. Suresh A. Thirumal Raj Shankargouda Patil |
| author_sort | Jaideep Mahendra |
| collection | DOAJ |
| description | Background: The existing data show that inflammasomes play a role in periodontal disease pathogenesis. However, their role in the pathogenesis of periodontitis and coronary heart disease remains unclear. This study had the objective of assessing NLRP3 (rs4612666) and CARD8 (rs2043211) gene polymorphisms in dental plaque and blood of generalized chronic periodontitis (CP) patients in the presence and absence of coronary heart disease (CHD). Methods: A total of 70 subjects were divided into two groups, including CP and CP + CHD subjects. Demographic variables, periodontal, and cardiac parameters were recorded from both groups. Subgingival plaque and blood samples were obtained from both groups and were subjected to further molecular analysis for NLRP3 (rs4612666) and CARD8 (rs2043211) expression and allele change using conventional polymerase chain reaction (PCR) and gene sequencing (Sanger’s method). Results: Amongst the demographic variables, age, and monthly income were statistically significant between the two groups. Plaque index (PI), clinical attachment level (CAL), high-density lipoprotein (HDL), and low density-lipoprotein (LDL) exhibited statistically significant levels between the two groups. NLRP3 (rs4612666) and CARD8 (rs2043211) genes showed a statistically significant association of allele change (frequency) among the groups. In general, when all of the parameters were compared to the allele change of the genes, statistically significant relationships were found between the two groups. Conclusions: The present study expressed an allele change of the investigated genes which could profoundly affect the pathobiology of the two diseases under investigation. |
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| last_indexed | 2024-03-09T04:50:07Z |
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| series | Biology |
| spelling | doaj.art-9e3268d7a6e14328aded9956ba39ed092023-12-03T13:11:11ZengMDPI AGBiology2079-77372021-06-0110759210.3390/biology10070592Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular DiseasesJaideep Mahendra0Abirami Nayaki Rao1Little Mahendra2Hytham N. Fageeh3Hammam Ibrahim Fageeh4Thodur Madapusi Balaji5Saranya Varadarajan6Raghunathan Jagannathan7Deepak Mehta8Venkata V. Suresh9A. Thirumal Raj10Shankargouda Patil11Department of Periodontology, Meenakshi Ammal Dental College and Hospital, Chennai 600095, IndiaDepartment of Periodontology, Meenakshi Ammal Dental College and Hospital, Chennai 600095, IndiaMaktoum Bin Hamdan Dental University College, Dubai 213620, United Arab EmiratesDepartment of Preventive Dental Science, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaDepartment of Preventive Dental Science, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaDepartment of Periodontology, Tagore Dental College and Hospital, Chennai 600127, IndiaDepartment of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Chennai 600130, IndiaDepartment of Periodontology, Tagore Dental College and Hospital, Chennai 600127, IndiaDepartment of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Miyagi 980-8575, JapanDepartment of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Miyagi 980-8575, JapanDepartment of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Chennai 600130, IndiaDepartment of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan 45142, Saudi ArabiaBackground: The existing data show that inflammasomes play a role in periodontal disease pathogenesis. However, their role in the pathogenesis of periodontitis and coronary heart disease remains unclear. This study had the objective of assessing NLRP3 (rs4612666) and CARD8 (rs2043211) gene polymorphisms in dental plaque and blood of generalized chronic periodontitis (CP) patients in the presence and absence of coronary heart disease (CHD). Methods: A total of 70 subjects were divided into two groups, including CP and CP + CHD subjects. Demographic variables, periodontal, and cardiac parameters were recorded from both groups. Subgingival plaque and blood samples were obtained from both groups and were subjected to further molecular analysis for NLRP3 (rs4612666) and CARD8 (rs2043211) expression and allele change using conventional polymerase chain reaction (PCR) and gene sequencing (Sanger’s method). Results: Amongst the demographic variables, age, and monthly income were statistically significant between the two groups. Plaque index (PI), clinical attachment level (CAL), high-density lipoprotein (HDL), and low density-lipoprotein (LDL) exhibited statistically significant levels between the two groups. NLRP3 (rs4612666) and CARD8 (rs2043211) genes showed a statistically significant association of allele change (frequency) among the groups. In general, when all of the parameters were compared to the allele change of the genes, statistically significant relationships were found between the two groups. Conclusions: The present study expressed an allele change of the investigated genes which could profoundly affect the pathobiology of the two diseases under investigation.https://www.mdpi.com/2079-7737/10/7/592cardiovascular diseasegenetic expressioninflammasomeperiodontal medicine |
| spellingShingle | Jaideep Mahendra Abirami Nayaki Rao Little Mahendra Hytham N. Fageeh Hammam Ibrahim Fageeh Thodur Madapusi Balaji Saranya Varadarajan Raghunathan Jagannathan Deepak Mehta Venkata V. Suresh A. Thirumal Raj Shankargouda Patil Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases Biology cardiovascular disease genetic expression inflammasome periodontal medicine |
| title | Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases |
| title_full | Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases |
| title_fullStr | Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases |
| title_full_unstemmed | Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases |
| title_short | Genetic Polymorphisms of NLRP3 (rs4612666) and CARD8 (rs2043211) in Periodontitis and Cardiovascular Diseases |
| title_sort | genetic polymorphisms of nlrp3 rs4612666 and card8 rs2043211 in periodontitis and cardiovascular diseases |
| topic | cardiovascular disease genetic expression inflammasome periodontal medicine |
| url | https://www.mdpi.com/2079-7737/10/7/592 |
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