Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitro
Hemostasis is achieved through the comprehensive effect of multiple pathways. A composite porous material was designed consisting of silk fibroin (SF), gelatin (GA) and calcium alginate (CA), integrating the good biocompatibility of SF, swelling property of GA and hemostatic factor Ca2+ of CA. Water...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522001988 |
| _version_ | 1811332209865392128 |
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| author | Mengnan Dai Meng Li Jiajun Gong Lingpeng Meng Boyu Zhang Yujin Zhang Yin Yin Jiannan Wang |
| author_facet | Mengnan Dai Meng Li Jiajun Gong Lingpeng Meng Boyu Zhang Yujin Zhang Yin Yin Jiannan Wang |
| author_sort | Mengnan Dai |
| collection | DOAJ |
| description | Hemostasis is achieved through the comprehensive effect of multiple pathways. A composite porous material was designed consisting of silk fibroin (SF), gelatin (GA) and calcium alginate (CA), integrating the good biocompatibility of SF, swelling property of GA and hemostatic factor Ca2+ of CA. Water absorption, platelet adhesion, fibrinogen adsorption, blood coagulation activity, and cytotoxicity were investigated. Pore size in SF/GA porous materials increased with increasing content of GA in SF but decreased significantly in SF/CA porous materials after incorporating CA in SF. Based on the water binding capacity of materials and capillary effect of pores, the binary composites SF/GA1:1 and SF/CA3:1 displayed the best water absorption. Although GA and CA were more favorable for fibrinogen adsorption, SF/GA1:1 and SF/CA1:1 exhibited greater numbers of adhered platelets, shorter clotting times, and greater whole blood coagulation ability. Human skin fibroblasts spread well on the pure SF and GA and all the composites of SF/GA and SF/CA, and they showed high proliferative activity. Pure CA inhibited cell proliferation. For ternary composite materials, the comprehensive hemostatic ability of SF/GA/CA1:1:1 was greater than that of SF/GA/CA1:1:3. |
| first_indexed | 2024-04-13T16:33:35Z |
| format | Article |
| id | doaj.art-9e32c88d3db74d4d874b85fd0b19f8c2 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-13T16:33:35Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-9e32c88d3db74d4d874b85fd0b19f8c22022-12-22T02:39:30ZengElsevierMaterials & Design0264-12752022-04-01216110577Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitroMengnan Dai0Meng Li1Jiajun Gong2Lingpeng Meng3Boyu Zhang4Yujin Zhang5Yin Yin6Jiannan Wang7National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, ChinaDepartment of Medicine, Soochow University, Suzhou, Jiangsu 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, China; Corresponding author at: Postal address: No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu 215123, China.Hemostasis is achieved through the comprehensive effect of multiple pathways. A composite porous material was designed consisting of silk fibroin (SF), gelatin (GA) and calcium alginate (CA), integrating the good biocompatibility of SF, swelling property of GA and hemostatic factor Ca2+ of CA. Water absorption, platelet adhesion, fibrinogen adsorption, blood coagulation activity, and cytotoxicity were investigated. Pore size in SF/GA porous materials increased with increasing content of GA in SF but decreased significantly in SF/CA porous materials after incorporating CA in SF. Based on the water binding capacity of materials and capillary effect of pores, the binary composites SF/GA1:1 and SF/CA3:1 displayed the best water absorption. Although GA and CA were more favorable for fibrinogen adsorption, SF/GA1:1 and SF/CA1:1 exhibited greater numbers of adhered platelets, shorter clotting times, and greater whole blood coagulation ability. Human skin fibroblasts spread well on the pure SF and GA and all the composites of SF/GA and SF/CA, and they showed high proliferative activity. Pure CA inhibited cell proliferation. For ternary composite materials, the comprehensive hemostatic ability of SF/GA/CA1:1:1 was greater than that of SF/GA/CA1:1:3.http://www.sciencedirect.com/science/article/pii/S0264127522001988Silk fibroinGelatinCalcium alginateComposite materialsHemostasis |
| spellingShingle | Mengnan Dai Meng Li Jiajun Gong Lingpeng Meng Boyu Zhang Yujin Zhang Yin Yin Jiannan Wang Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitro Materials & Design Silk fibroin Gelatin Calcium alginate Composite materials Hemostasis |
| title | Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitro |
| title_full | Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitro |
| title_fullStr | Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitro |
| title_full_unstemmed | Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitro |
| title_short | Silk fibroin/gelatin/calcium alginate composite materials: Preparation, pore characteristics, comprehensive hemostasis in vitro |
| title_sort | silk fibroin gelatin calcium alginate composite materials preparation pore characteristics comprehensive hemostasis in vitro |
| topic | Silk fibroin Gelatin Calcium alginate Composite materials Hemostasis |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522001988 |
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