Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform
The current study presents a highly sensitive and selective surface-enhanced Raman scattering (SERS) based method for detecting Staphylococcus aureus (S. aureus) in food and water samples. The bioassay includes target bacteria recognition by a specific aptamer complex, fast syringe filtration and th...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
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| Series: | Journal of Science: Advanced Materials and Devices |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468217924000212 |
| _version_ | 1797311528773353472 |
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| author | Divagar Muthukumar Giorgi Shtenberg |
| author_facet | Divagar Muthukumar Giorgi Shtenberg |
| author_sort | Divagar Muthukumar |
| collection | DOAJ |
| description | The current study presents a highly sensitive and selective surface-enhanced Raman scattering (SERS) based method for detecting Staphylococcus aureus (S. aureus) in food and water samples. The bioassay includes target bacteria recognition by a specific aptamer complex, fast syringe filtration and the assessment of unbound biorecognition complexes using a silver-coated porous silicon SERS platform. Notably, the developed bioassay showed high sensitivity toward S. aureus detection, achieving an impressive detection limit of 2 CFU mL−1 and a broad linear response of 101–106 CFU mL−1. Furthermore, the selectivity, regeneration and overall shelf-life were thoroughly evaluated while depicting satisfactory performances. Finally, the practicality of the developed SERS bioassay was elucidated in various samples (i.e., fish, milk, groundwater and tahini) spiked with different S. aureus concentrations that revealed recovery values of 93–110%. The successful validation of actual samples' analysis emphasizes the platform's reliability, robustness and suitability for practical use, including on-site operation. |
| first_indexed | 2024-03-08T02:01:02Z |
| format | Article |
| id | doaj.art-9e33e2d811454a1abd7d01cbf71ca44d |
| institution | Directory Open Access Journal |
| issn | 2468-2179 |
| language | English |
| last_indexed | 2024-03-08T02:01:02Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Science: Advanced Materials and Devices |
| spelling | doaj.art-9e33e2d811454a1abd7d01cbf71ca44d2024-02-14T05:18:34ZengElsevierJournal of Science: Advanced Materials and Devices2468-21792024-06-0192100690Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platformDivagar Muthukumar0Giorgi Shtenberg1Institute of Agricultural Engineering, ARO, Volcani Institute, Rishon LeZion, IsraelCorresponding author.; Institute of Agricultural Engineering, ARO, Volcani Institute, Rishon LeZion, IsraelThe current study presents a highly sensitive and selective surface-enhanced Raman scattering (SERS) based method for detecting Staphylococcus aureus (S. aureus) in food and water samples. The bioassay includes target bacteria recognition by a specific aptamer complex, fast syringe filtration and the assessment of unbound biorecognition complexes using a silver-coated porous silicon SERS platform. Notably, the developed bioassay showed high sensitivity toward S. aureus detection, achieving an impressive detection limit of 2 CFU mL−1 and a broad linear response of 101–106 CFU mL−1. Furthermore, the selectivity, regeneration and overall shelf-life were thoroughly evaluated while depicting satisfactory performances. Finally, the practicality of the developed SERS bioassay was elucidated in various samples (i.e., fish, milk, groundwater and tahini) spiked with different S. aureus concentrations that revealed recovery values of 93–110%. The successful validation of actual samples' analysis emphasizes the platform's reliability, robustness and suitability for practical use, including on-site operation.http://www.sciencedirect.com/science/article/pii/S2468217924000212AptamerFood safetyPorous siliconS. aureusSERS |
| spellingShingle | Divagar Muthukumar Giorgi Shtenberg Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform Journal of Science: Advanced Materials and Devices Aptamer Food safety Porous silicon S. aureus SERS |
| title | Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform |
| title_full | Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform |
| title_fullStr | Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform |
| title_full_unstemmed | Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform |
| title_short | Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform |
| title_sort | quantitative detection of staphylococcus aureus using aptamer based bioassay coupled with porous si sers platform |
| topic | Aptamer Food safety Porous silicon S. aureus SERS |
| url | http://www.sciencedirect.com/science/article/pii/S2468217924000212 |
| work_keys_str_mv | AT divagarmuthukumar quantitativedetectionofstaphylococcusaureususingaptamerbasedbioassaycoupledwithporoussisersplatform AT giorgishtenberg quantitativedetectionofstaphylococcusaureususingaptamerbasedbioassaycoupledwithporoussisersplatform |