iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment
Rhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at the cellular level during RRD, providing additional...
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MDPI AG
2018-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/19/4/1157 |
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| author | Fátima Milhano Santos Leonor Mesquita Gaspar Sergio Ciordia Ana Sílvia Rocha João Paulo Castro e Sousa Alberto Paradela Luís António Passarinha Cândida Teixeira Tomaz |
| author_facet | Fátima Milhano Santos Leonor Mesquita Gaspar Sergio Ciordia Ana Sílvia Rocha João Paulo Castro e Sousa Alberto Paradela Luís António Passarinha Cândida Teixeira Tomaz |
| author_sort | Fátima Milhano Santos |
| collection | DOAJ |
| description | Rhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at the cellular level during RRD, providing additional information about the molecular mechanisms underlying its pathogenesis. In the present study, iTRAQ labeling was combined with two-dimensional LC-ESI-MS/MS to find expression changes in the proteome of vitreous from patients with RRD when compared to control samples. A total of 150 proteins were found differentially expressed in the vitreous of patients with RRD, including 96 overexpressed and 54 underexpressed. Several overexpressed proteins, several such as glycolytic enzymes (fructose-bisphosphate aldolase A, gamma-enolase, and phosphoglycerate kinase 1), glucose transporters (GLUT-1), growth factors (metalloproteinase inhibitor 1), and serine protease inhibitors (plasminogen activator inhibitor 1) are regulated by HIF-1, which suggests that HIF-1 signaling pathway can be triggered in response to RRD. Also, the accumulation of photoreceptor proteins, including phosducin, rhodopsin, and s-arrestin, and vimentin in vitreous may indicate that photoreceptor degeneration occurs in RRD. Also, the accumulation of photoreceptor proteins, including phosducin, rhodopsin, and s-arrestin, and vimentin in vitreous may indicate that photoreceptor degeneration occurs in RRD. Nevertheless, the differentially expressed proteins found in this study suggest that different mechanisms are activated after RRD to promote the survival of retinal cells through complex cellular responses. |
| first_indexed | 2024-04-12T19:27:42Z |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T19:27:42Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9e34933e21bd4438be6035c74bf064e72022-12-22T03:19:27ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-04-01194115710.3390/ijms19041157ijms19041157iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal DetachmentFátima Milhano Santos0Leonor Mesquita Gaspar1Sergio Ciordia2Ana Sílvia Rocha3João Paulo Castro e Sousa4Alberto Paradela5Luís António Passarinha6Cândida Teixeira Tomaz7CICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalUnidad de Proteomica, Centro Nacional de Biotecnología, CSIC, Calle Darwin 3, Campus de Cantoblanco, 28049 Madrid, SpainCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalUnidad de Proteomica, Centro Nacional de Biotecnología, CSIC, Calle Darwin 3, Campus de Cantoblanco, 28049 Madrid, SpainCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalRhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at the cellular level during RRD, providing additional information about the molecular mechanisms underlying its pathogenesis. In the present study, iTRAQ labeling was combined with two-dimensional LC-ESI-MS/MS to find expression changes in the proteome of vitreous from patients with RRD when compared to control samples. A total of 150 proteins were found differentially expressed in the vitreous of patients with RRD, including 96 overexpressed and 54 underexpressed. Several overexpressed proteins, several such as glycolytic enzymes (fructose-bisphosphate aldolase A, gamma-enolase, and phosphoglycerate kinase 1), glucose transporters (GLUT-1), growth factors (metalloproteinase inhibitor 1), and serine protease inhibitors (plasminogen activator inhibitor 1) are regulated by HIF-1, which suggests that HIF-1 signaling pathway can be triggered in response to RRD. Also, the accumulation of photoreceptor proteins, including phosducin, rhodopsin, and s-arrestin, and vimentin in vitreous may indicate that photoreceptor degeneration occurs in RRD. Also, the accumulation of photoreceptor proteins, including phosducin, rhodopsin, and s-arrestin, and vimentin in vitreous may indicate that photoreceptor degeneration occurs in RRD. Nevertheless, the differentially expressed proteins found in this study suggest that different mechanisms are activated after RRD to promote the survival of retinal cells through complex cellular responses.http://www.mdpi.com/1422-0067/19/4/1157iTRAQquantitative proteomicsretinal detachmentvitreous proteome |
| spellingShingle | Fátima Milhano Santos Leonor Mesquita Gaspar Sergio Ciordia Ana Sílvia Rocha João Paulo Castro e Sousa Alberto Paradela Luís António Passarinha Cândida Teixeira Tomaz iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment International Journal of Molecular Sciences iTRAQ quantitative proteomics retinal detachment vitreous proteome |
| title | iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment |
| title_full | iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment |
| title_fullStr | iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment |
| title_full_unstemmed | iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment |
| title_short | iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment |
| title_sort | itraq quantitative proteomic analysis of vitreous from patients with retinal detachment |
| topic | iTRAQ quantitative proteomics retinal detachment vitreous proteome |
| url | http://www.mdpi.com/1422-0067/19/4/1157 |
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