Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, Iran
Background: The relationship between hepatic disorders and Transfusion transmitted virus (TTV) and the significance of parenteral transmission of TTV are controversial. The aim of this study was to compare the prevalence of TTV in injection drug users (IDUs), hemodialysis patients, and healthy blood...
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| Format: | Article |
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Isfahan University of Medical Sciences
2012-05-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/948 |
| _version_ | 1797722148484153344 |
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| author | Behrooz Ataei Alireza Emami Naeini Farzin Khorvash Mohammad Reza Yazdani Abbas Ali Javadi Mojtaba Akbari Mehdi Ataie |
| author_facet | Behrooz Ataei Alireza Emami Naeini Farzin Khorvash Mohammad Reza Yazdani Abbas Ali Javadi Mojtaba Akbari Mehdi Ataie |
| author_sort | Behrooz Ataei |
| collection | DOAJ |
| description | Background: The relationship between hepatic disorders and Transfusion transmitted virus (TTV) and the significance of parenteral transmission of TTV are controversial. The aim of this study was to compare the prevalence of TTV in injection drug users (IDUs), hemodialysis patients, and healthy blood donors, in Isfahan, Iran, at the year of 2010.
Methods: In a case-control study, 108 subjects included 36 hemodialysis patients, 36 IDUs, and 36 healthy blood donors (as control group) were studied. Five-mL blood sample was taken from each subject on EDTA-containing tubes. Then DNA extraction was implemented using DNA extraction kit, and DNA reproduction was done using specific primers and probe by Rotor-Gene 6000 real-time PCR instrument.
Findings: The mean of age in subjects was 38.7 ± 14.7 and 71 (66%) were male. Of 108 cases, 30 (27.8%) were TTV positive and 78 (72.2%) were TTV negative. The prevalence of TTV in IDUs 21 (58%) and hemodialysis patients 6 (17%) was higher than healthy blood donors 3 (8%) that was statistically significant (P < 0.0001).
Conclusion: The prevalence of TTV in IDUs and hemodialysis patients is significantly higher than the general population in Isfahan, Iran. It seems necessary to make plans and policies to reduce the risk of TTV transmission to families of hemodialysis patient and IDUs, individuals who have close contact with them and health care workers.
Keywords: Transfusion transmitted virus, Hemodialysis patient, Injection drug user, Blood donor |
| first_indexed | 2024-03-12T09:43:24Z |
| format | Article |
| id | doaj.art-9e3ca585260742edad28aca4aae89cca |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:43:24Z |
| publishDate | 2012-05-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-9e3ca585260742edad28aca4aae89cca2023-09-02T13:05:54ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2012-05-01301831035Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, IranBehrooz Ataei0Alireza Emami Naeini1Farzin Khorvash2Mohammad Reza Yazdani3Abbas Ali Javadi4Mojtaba Akbari5Mehdi Ataie6Associated Professor, Department of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Infectious Diseases, Nosocomial Infections Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssociated Professor, Department of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssociated Professor, Department of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranEpidemiologist, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranStudent of Medicine, School of Medicine, Najafabad Branch, Islamic Azad University, Isfahan, IranBackground: The relationship between hepatic disorders and Transfusion transmitted virus (TTV) and the significance of parenteral transmission of TTV are controversial. The aim of this study was to compare the prevalence of TTV in injection drug users (IDUs), hemodialysis patients, and healthy blood donors, in Isfahan, Iran, at the year of 2010. Methods: In a case-control study, 108 subjects included 36 hemodialysis patients, 36 IDUs, and 36 healthy blood donors (as control group) were studied. Five-mL blood sample was taken from each subject on EDTA-containing tubes. Then DNA extraction was implemented using DNA extraction kit, and DNA reproduction was done using specific primers and probe by Rotor-Gene 6000 real-time PCR instrument. Findings: The mean of age in subjects was 38.7 ± 14.7 and 71 (66%) were male. Of 108 cases, 30 (27.8%) were TTV positive and 78 (72.2%) were TTV negative. The prevalence of TTV in IDUs 21 (58%) and hemodialysis patients 6 (17%) was higher than healthy blood donors 3 (8%) that was statistically significant (P < 0.0001). Conclusion: The prevalence of TTV in IDUs and hemodialysis patients is significantly higher than the general population in Isfahan, Iran. It seems necessary to make plans and policies to reduce the risk of TTV transmission to families of hemodialysis patient and IDUs, individuals who have close contact with them and health care workers. Keywords: Transfusion transmitted virus, Hemodialysis patient, Injection drug user, Blood donorhttp://jims.mui.ac.ir/index.php/jims/article/view/948 |
| spellingShingle | Behrooz Ataei Alireza Emami Naeini Farzin Khorvash Mohammad Reza Yazdani Abbas Ali Javadi Mojtaba Akbari Mehdi Ataie Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, Iran مجله دانشکده پزشکی اصفهان |
| title | Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, Iran |
| title_full | Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, Iran |
| title_fullStr | Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, Iran |
| title_full_unstemmed | Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, Iran |
| title_short | Prevalence of Transfusion Transmitted Virus (TTV) Infection in Injection Drug Users and Hemodialysis Patients Compare to Healthy Blood Donors Using RT-PCR Method in Isfahan, Iran |
| title_sort | prevalence of transfusion transmitted virus ttv infection in injection drug users and hemodialysis patients compare to healthy blood donors using rt pcr method in isfahan iran |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/948 |
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