Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma
Abstract Exosomes are a new way of the communication between the tumor cell and macrophage in the micro-environment. The macrophage can be induced to different phenotypes according to the different tumors. In the present study, long-chain noncoding RNA HOTAIR (lncRNA HOTAIR) was highly expressed in...
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| Format: | Article |
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BMC
2022-11-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-022-10210-5 |
| _version_ | 1811186964745945088 |
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| author | Jingting Wang Nan Wang Zeyu Zheng Yanlu Che Masanobu Suzuki Satoshi Kano Jianguang Lu Peng Wang Yanan Sun Akihiro Homma |
| author_facet | Jingting Wang Nan Wang Zeyu Zheng Yanlu Che Masanobu Suzuki Satoshi Kano Jianguang Lu Peng Wang Yanan Sun Akihiro Homma |
| author_sort | Jingting Wang |
| collection | DOAJ |
| description | Abstract Exosomes are a new way of the communication between the tumor cell and macrophage in the micro-environment. The macrophage can be induced to different phenotypes according to the different tumors. In the present study, long-chain noncoding RNA HOTAIR (lncRNA HOTAIR) was highly expressed in LSCC and exosomes. The pathway of exosomal lncRNA HOTAIR inducing macrophage to M2 polarization in the LSCC was investigated. The carcinoma tissues and adjacent tissues were collected from 104 LSCC cases, and the positive relationship between CD163-/CD206-M2 macrophage infiltration and clinical phase, lymph node spreading and pathological phase in LSCC was observed. To examine the role of exosomal lncRNA HOTAIR, macrophages were co-cultured with LSCC-exosomes of high lncRNA HOTAIR expression or transferred with HOTAIR mimics. It was suggested that exosomal lncRNA HOTAIR can induce macrophages to M2 polarization by PI3K/p-AKT/AKT signaling pathway. Furthermore, exo-treated M2 macrophages facilitate the migration, proliferation, and EMT of LSCC. |
| first_indexed | 2024-04-11T13:53:59Z |
| format | Article |
| id | doaj.art-9e3ca5b2d4b44565a352f97a805eabd3 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-11T13:53:59Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-9e3ca5b2d4b44565a352f97a805eabd32022-12-22T04:20:24ZengBMCBMC Cancer1471-24072022-11-0122111310.1186/s12885-022-10210-5Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinomaJingting Wang0Nan Wang1Zeyu Zheng2Yanlu Che3Masanobu Suzuki4Satoshi Kano5Jianguang Lu6Peng Wang7Yanan Sun8Akihiro Homma9Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical UniversityDepartment of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical UniversityDepartment of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical UniversityDepartment of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical UniversityDepartment of Otolaryngology-Head and Neck Surgery, Hokkaido University Graduate School of MedicineDepartment of Otolaryngology-Head and Neck Surgery, Hokkaido University Graduate School of MedicineDepartment of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical UniversityDepartment of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical UniversityDepartment of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical UniversityDepartment of Otolaryngology-Head and Neck Surgery, Hokkaido University Graduate School of MedicineAbstract Exosomes are a new way of the communication between the tumor cell and macrophage in the micro-environment. The macrophage can be induced to different phenotypes according to the different tumors. In the present study, long-chain noncoding RNA HOTAIR (lncRNA HOTAIR) was highly expressed in LSCC and exosomes. The pathway of exosomal lncRNA HOTAIR inducing macrophage to M2 polarization in the LSCC was investigated. The carcinoma tissues and adjacent tissues were collected from 104 LSCC cases, and the positive relationship between CD163-/CD206-M2 macrophage infiltration and clinical phase, lymph node spreading and pathological phase in LSCC was observed. To examine the role of exosomal lncRNA HOTAIR, macrophages were co-cultured with LSCC-exosomes of high lncRNA HOTAIR expression or transferred with HOTAIR mimics. It was suggested that exosomal lncRNA HOTAIR can induce macrophages to M2 polarization by PI3K/p-AKT/AKT signaling pathway. Furthermore, exo-treated M2 macrophages facilitate the migration, proliferation, and EMT of LSCC.https://doi.org/10.1186/s12885-022-10210-5lncRNA HOTAIRExosomesMacrophageLSCCEMT |
| spellingShingle | Jingting Wang Nan Wang Zeyu Zheng Yanlu Che Masanobu Suzuki Satoshi Kano Jianguang Lu Peng Wang Yanan Sun Akihiro Homma Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma BMC Cancer lncRNA HOTAIR Exosomes Macrophage LSCC EMT |
| title | Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma |
| title_full | Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma |
| title_fullStr | Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma |
| title_full_unstemmed | Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma |
| title_short | Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma |
| title_sort | exosomal lncrna hotair induce macrophages to m2 polarization via pi3k p akt akt pathway and promote emt and metastasis in laryngeal squamous cell carcinoma |
| topic | lncRNA HOTAIR Exosomes Macrophage LSCC EMT |
| url | https://doi.org/10.1186/s12885-022-10210-5 |
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