Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources
Abstract The European flat oyster (Ostrea edulis) is a highly appreciated mollusk with an important aquaculture production throughout the 20th century, in addition to playing an important role on coastal ecosystems. Overexploitation of natural beds, habitat degradation, introduction of non‐native sp...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-10-01
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| Series: | Evolutionary Applications |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/eva.12832 |
| _version_ | 1818107821096435712 |
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| author | Manuel Vera Belén G. Pardo Asunción Cao Román Vilas Carlos Fernández Andrés Blanco Alejandro P. Gutierrez Tim P. Bean Ross D. Houston Antonio Villalba Paulino Martínez |
| author_facet | Manuel Vera Belén G. Pardo Asunción Cao Román Vilas Carlos Fernández Andrés Blanco Alejandro P. Gutierrez Tim P. Bean Ross D. Houston Antonio Villalba Paulino Martínez |
| author_sort | Manuel Vera |
| collection | DOAJ |
| description | Abstract The European flat oyster (Ostrea edulis) is a highly appreciated mollusk with an important aquaculture production throughout the 20th century, in addition to playing an important role on coastal ecosystems. Overexploitation of natural beds, habitat degradation, introduction of non‐native species, and epidemic outbreaks have severely affected this important resource, particularly, the protozoan parasite Bonamia ostreae, which is the main concern affecting its production and conservation. In order to identify genomic regions and markers potentially associated with bonamiosis resistance, six oyster beds distributed throughout the European Atlantic coast were sampled. Three of them have been exposed to this parasite since the early 1980s and showed some degree of innate resistance (long‐term affected group, LTA), while the other three were free of B. ostreae at least until sampling date (naïve group, NV). A total of 14,065 SNPs were analyzed, including 37 markers from candidate genes and 14,028 from a medium‐density SNP array. Gene diversity was similar between LTA and NV groups suggesting no genetic erosion due to long‐term exposure to the parasite, and three population clusters were detected using the whole dataset. Tests for divergent selection between NV and LTA groups detected the presence of a very consistent set of 22 markers, located within a putative single genomic region, which suggests the presence of a major quantitative trait locus associated with B. ostreae resistance. Moreover, 324 outlier loci associated with factors other than bonamiosis were identified allowing fully discrimination of all the oyster beds. A practical tool which included the 84 highest discriminative markers for tracing O. edulis populations was developed and tested with empirical data. Results reported herein could assist the production of stocks with improved resistance to bonamiosis and facilitate the management of oyster beds for recovery production and ecosystem services provided by this species. |
| first_indexed | 2024-12-11T02:05:33Z |
| format | Article |
| id | doaj.art-9e3f4c34012c4b67a34231ad0badf2b4 |
| institution | Directory Open Access Journal |
| issn | 1752-4571 |
| language | English |
| last_indexed | 2024-12-11T02:05:33Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Evolutionary Applications |
| spelling | doaj.art-9e3f4c34012c4b67a34231ad0badf2b42022-12-22T01:24:23ZengWileyEvolutionary Applications1752-45712019-10-011291781179610.1111/eva.12832Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resourcesManuel Vera0Belén G. Pardo1Asunción Cao2Román Vilas3Carlos Fernández4Andrés Blanco5Alejandro P. Gutierrez6Tim P. Bean7Ross D. Houston8Antonio Villalba9Paulino Martínez10Department of Zoology, Genetics and Physical Anthropology, ACUIGEN group, Faculty of Veterinary Universidade de Santiago de Compostela Lugo SpainDepartment of Zoology, Genetics and Physical Anthropology, ACUIGEN group, Faculty of Veterinary Universidade de Santiago de Compostela Lugo SpainCentro de Investigacións Mariñas (CIMA) Consellería do Mar, Xunta de Galicia Pontevedra SpainDepartment of Zoology, Genetics and Physical Anthropology, ACUIGEN group, Faculty of Veterinary Universidade de Santiago de Compostela Lugo SpainDepartment of Zoology, Genetics and Physical Anthropology, ACUIGEN group, Faculty of Veterinary Universidade de Santiago de Compostela Lugo SpainDepartment of Zoology, Genetics and Physical Anthropology, ACUIGEN group, Faculty of Veterinary Universidade de Santiago de Compostela Lugo SpainThe Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh Midlothian UKThe Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh Midlothian UKThe Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh Midlothian UKCentro de Investigacións Mariñas (CIMA) Consellería do Mar, Xunta de Galicia Pontevedra SpainDepartment of Zoology, Genetics and Physical Anthropology, ACUIGEN group, Faculty of Veterinary Universidade de Santiago de Compostela Lugo SpainAbstract The European flat oyster (Ostrea edulis) is a highly appreciated mollusk with an important aquaculture production throughout the 20th century, in addition to playing an important role on coastal ecosystems. Overexploitation of natural beds, habitat degradation, introduction of non‐native species, and epidemic outbreaks have severely affected this important resource, particularly, the protozoan parasite Bonamia ostreae, which is the main concern affecting its production and conservation. In order to identify genomic regions and markers potentially associated with bonamiosis resistance, six oyster beds distributed throughout the European Atlantic coast were sampled. Three of them have been exposed to this parasite since the early 1980s and showed some degree of innate resistance (long‐term affected group, LTA), while the other three were free of B. ostreae at least until sampling date (naïve group, NV). A total of 14,065 SNPs were analyzed, including 37 markers from candidate genes and 14,028 from a medium‐density SNP array. Gene diversity was similar between LTA and NV groups suggesting no genetic erosion due to long‐term exposure to the parasite, and three population clusters were detected using the whole dataset. Tests for divergent selection between NV and LTA groups detected the presence of a very consistent set of 22 markers, located within a putative single genomic region, which suggests the presence of a major quantitative trait locus associated with B. ostreae resistance. Moreover, 324 outlier loci associated with factors other than bonamiosis were identified allowing fully discrimination of all the oyster beds. A practical tool which included the 84 highest discriminative markers for tracing O. edulis populations was developed and tested with empirical data. Results reported herein could assist the production of stocks with improved resistance to bonamiosis and facilitate the management of oyster beds for recovery production and ecosystem services provided by this species.https://doi.org/10.1111/eva.12832Bonamia ostreaecandidate genesdisease resistancedivergent selectiongenetic traceabilityOstrea edulis |
| spellingShingle | Manuel Vera Belén G. Pardo Asunción Cao Román Vilas Carlos Fernández Andrés Blanco Alejandro P. Gutierrez Tim P. Bean Ross D. Houston Antonio Villalba Paulino Martínez Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources Evolutionary Applications Bonamia ostreae candidate genes disease resistance divergent selection genetic traceability Ostrea edulis |
| title | Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources |
| title_full | Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources |
| title_fullStr | Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources |
| title_full_unstemmed | Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources |
| title_short | Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources |
| title_sort | signatures of selection for bonamiosis resistance in european flat oyster ostrea edulis new genomic tools for breeding programs and management of natural resources |
| topic | Bonamia ostreae candidate genes disease resistance divergent selection genetic traceability Ostrea edulis |
| url | https://doi.org/10.1111/eva.12832 |
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