Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase
The minor coat protein G3p of bacteriophage M13 is the key component for the host interaction of this virus and binds to <i>Escherichia coli</i> at the tip of the F pili. As we show here, during the biosynthesis of G3p as a preprotein, the signal sequence interacts primarily with SecY, w...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/7/1414 |
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| author | Farina Kleinbeck Andreas Kuhn |
| author_facet | Farina Kleinbeck Andreas Kuhn |
| author_sort | Farina Kleinbeck |
| collection | DOAJ |
| description | The minor coat protein G3p of bacteriophage M13 is the key component for the host interaction of this virus and binds to <i>Escherichia coli</i> at the tip of the F pili. As we show here, during the biosynthesis of G3p as a preprotein, the signal sequence interacts primarily with SecY, whereas the hydrophobic anchor sequence at the C-terminus interacts with YidC. Using arrested nascent chains and thiol crosslinking, we show here that the ribosome-exposed signal sequence is first contacted by SecY but not by YidC, suggesting that only SecYEG is involved at this early stage. The protein has a large periplasmic domain, a hydrophobic anchor sequence of 21 residues and a short C-terminal tail that remains in the cytoplasm. During the later synthesis of the entire G3p, the residues 387, 389 and 392 in anchor domain contact YidC in its hydrophobic slide to hold translocation of the C-terminal tail. Finally, the protein is processed by leader peptidase and assembled into new progeny phage particles that are extruded out of the cell. |
| first_indexed | 2024-03-10T09:20:01Z |
| format | Article |
| id | doaj.art-9e401f79e52e4873b9f8e607cac5e71f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T09:20:01Z |
| publishDate | 2021-07-01 |
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| spelling | doaj.art-9e401f79e52e4873b9f8e607cac5e71f2023-11-22T05:15:38ZengMDPI AGViruses1999-49152021-07-01137141410.3390/v13071414Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG TranslocaseFarina Kleinbeck0Andreas Kuhn1Institute of Biology, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Biology, University of Hohenheim, 70599 Stuttgart, GermanyThe minor coat protein G3p of bacteriophage M13 is the key component for the host interaction of this virus and binds to <i>Escherichia coli</i> at the tip of the F pili. As we show here, during the biosynthesis of G3p as a preprotein, the signal sequence interacts primarily with SecY, whereas the hydrophobic anchor sequence at the C-terminus interacts with YidC. Using arrested nascent chains and thiol crosslinking, we show here that the ribosome-exposed signal sequence is first contacted by SecY but not by YidC, suggesting that only SecYEG is involved at this early stage. The protein has a large periplasmic domain, a hydrophobic anchor sequence of 21 residues and a short C-terminal tail that remains in the cytoplasm. During the later synthesis of the entire G3p, the residues 387, 389 and 392 in anchor domain contact YidC in its hydrophobic slide to hold translocation of the C-terminal tail. Finally, the protein is processed by leader peptidase and assembled into new progeny phage particles that are extruded out of the cell.https://www.mdpi.com/1999-4915/13/7/1414bacteriophage M13membrane protein insertiontranslocase SecYEGmembrane insertase YidCmembrane potentialphage assembly |
| spellingShingle | Farina Kleinbeck Andreas Kuhn Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase Viruses bacteriophage M13 membrane protein insertion translocase SecYEG membrane insertase YidC membrane potential phage assembly |
| title | Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase |
| title_full | Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase |
| title_fullStr | Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase |
| title_full_unstemmed | Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase |
| title_short | Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase |
| title_sort | membrane insertion of the m13 minor coat protein g3p is dependent on yidc and the secayeg translocase |
| topic | bacteriophage M13 membrane protein insertion translocase SecYEG membrane insertase YidC membrane potential phage assembly |
| url | https://www.mdpi.com/1999-4915/13/7/1414 |
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