A CRISPR/LbCas12a-based method for detection of bacterial fruit blotch pathogens in watermelon

A CRISPR/LbCas12a-based method for detection of bacterial fruit blotch pathogens in watermelon

ABSTRACTAcidovorax citrulli is the main pathogen causing bacterial fruit blotch, which seriously threatens the global watermelon industry. At present, rapid, sensitive, and low-cost detection methods are urgently needed. The established CRISPR/LbCas12a visual detection method can specifically detect...

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Bibliographic Details
Main Authors: Zelu Wang, Wenhui Cheng, Zhiyu Dong, Xiamei Yao, Xu Deng, Chun Ou
Format: Article
Language:English
Published: American Society for Microbiology 2024-03-01
Series:Microbiology Spectrum
Subjects:
CRISPR/LbCas12a
Acidovorax citrulli
free-amplification
visualization
specificity
sensitivity
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03846-23

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