The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms
Summary: In nature, bacteria reside in biofilms– multicellular differentiated communities held together by an extracellular matrix. This work identified a novel subpopulation—mineral-forming cells—that is essential for biofilm formation in Bacillus subtilis biofilms. This subpopulation contains an i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422200579X |
| _version_ | 1811340618585079808 |
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| author | Alona Keren-Paz Harsh Maan Iris Karunker Tsviya Olender Sergey Kapishnikov Simon Dersch Elena Kartvelishvily Sharon G. Wolf Assaf Gal Peter L. Graumann Ilana Kolodkin-Gal |
| author_facet | Alona Keren-Paz Harsh Maan Iris Karunker Tsviya Olender Sergey Kapishnikov Simon Dersch Elena Kartvelishvily Sharon G. Wolf Assaf Gal Peter L. Graumann Ilana Kolodkin-Gal |
| author_sort | Alona Keren-Paz |
| collection | DOAJ |
| description | Summary: In nature, bacteria reside in biofilms– multicellular differentiated communities held together by an extracellular matrix. This work identified a novel subpopulation—mineral-forming cells—that is essential for biofilm formation in Bacillus subtilis biofilms. This subpopulation contains an intracellular calcium-accumulating niche, in which the formation of a calcium carbonate mineral is initiated. As the biofilm colony develops, this mineral grows in a controlled manner, forming a functional macrostructure that serves the entire community. Consistently, biofilm development is prevented by the inhibition of calcium uptake. Our results provide a clear demonstration of the orchestrated production of calcite exoskeleton, critical to morphogenesis in simple prokaryotes. |
| first_indexed | 2024-04-13T18:44:39Z |
| format | Article |
| id | doaj.art-9e45433b92194b29ae79997bf48bdab9 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-13T18:44:39Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9e45433b92194b29ae79997bf48bdab92022-12-22T02:34:38ZengElsevieriScience2589-00422022-06-01256104308The roles of intracellular and extracellular calcium in Bacillus subtilis biofilmsAlona Keren-Paz0Harsh Maan1Iris Karunker2Tsviya Olender3Sergey Kapishnikov4Simon Dersch5Elena Kartvelishvily6Sharon G. Wolf7Assaf Gal8Peter L. Graumann9Ilana Kolodkin-Gal10Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, IsraelChemical Research Support, Weizmann Institute of Science, Rehovot, IsraelCentre for Synthetic Microbiology (SYNMIKRO), Fachbereich Chemie, Philipps-Universität Marburg, Marburg, GermanyChemical Research Support, Weizmann Institute of Science, Rehovot, IsraelChemical Research Support, Weizmann Institute of Science, Rehovot, IsraelDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, IsraelCentre for Synthetic Microbiology (SYNMIKRO), Fachbereich Chemie, Philipps-Universität Marburg, Marburg, GermanyDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel; Department of Plant Pathology and Microbiology, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel; Corresponding authorSummary: In nature, bacteria reside in biofilms– multicellular differentiated communities held together by an extracellular matrix. This work identified a novel subpopulation—mineral-forming cells—that is essential for biofilm formation in Bacillus subtilis biofilms. This subpopulation contains an intracellular calcium-accumulating niche, in which the formation of a calcium carbonate mineral is initiated. As the biofilm colony develops, this mineral grows in a controlled manner, forming a functional macrostructure that serves the entire community. Consistently, biofilm development is prevented by the inhibition of calcium uptake. Our results provide a clear demonstration of the orchestrated production of calcite exoskeleton, critical to morphogenesis in simple prokaryotes.http://www.sciencedirect.com/science/article/pii/S258900422200579XMicrobiologyMicrobiofilmscell biology |
| spellingShingle | Alona Keren-Paz Harsh Maan Iris Karunker Tsviya Olender Sergey Kapishnikov Simon Dersch Elena Kartvelishvily Sharon G. Wolf Assaf Gal Peter L. Graumann Ilana Kolodkin-Gal The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms iScience Microbiology Microbiofilms cell biology |
| title | The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms |
| title_full | The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms |
| title_fullStr | The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms |
| title_full_unstemmed | The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms |
| title_short | The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms |
| title_sort | roles of intracellular and extracellular calcium in bacillus subtilis biofilms |
| topic | Microbiology Microbiofilms cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S258900422200579X |
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