Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes
In this manuscript, we report the proteins macrophage infectivity potentiator (mip, CAB080), major outer membrane protein (momp, CAB048), and polymorphic outer membrane protein (pmp18D, CAB776) that are expressed in different times of pregnancy in mice infected with Chlamydia abortus. Enzootic abort...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2022-03-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.33073/pjm-2022-014 |
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| author | Arif Eman Dhahir Saeed Nahla Mohammad Rachid Shwan Kamal Dyary Hiewa Othman Rashid Peshnyar M.A. |
| author_facet | Arif Eman Dhahir Saeed Nahla Mohammad Rachid Shwan Kamal Dyary Hiewa Othman Rashid Peshnyar M.A. |
| author_sort | Arif Eman Dhahir |
| collection | DOAJ |
| description | In this manuscript, we report the proteins macrophage infectivity potentiator (mip, CAB080), major outer membrane protein (momp, CAB048), and polymorphic outer membrane protein (pmp18D, CAB776) that are expressed in different times of pregnancy in mice infected with Chlamydia abortus. Enzootic abortion of ewes (EAE) by C. abortus, an obligate intracellular pathogen, is a critical zoonotic disease-causing significant economic loss to livestock farming globally. This study was carried out for the detection and characterization of macrophage infectivity potentiator (mip, CAB080), major outer membrane protein (momp, CAB048), and polymorphic outer membrane protein (pmp18D, CAB776) using RT-qPCR. These proteins are believed to be expressed as virulence factors in C. abortus isolated from aborted ewes. BALB/c mice (pregnant and nonpregnant) were used as an animal model to be injected intraperitoneally with C. abortus culture in Vero cells since the endometrial lymphoid tissues of these animals resembles that of ewes. Also, the short duration of pregnancy in mice makes them a suitable animal model for obstetric studies. Tissue samples were taken from the mice after 10, 15, and 20 days of pregnancy to compare the expression of the genes mip, pmp18D, and ompA. Transcription level was quantified using RT-qPCR, the GAPDH transcription quantification, as a normalization signal. Abortion occurred in pregnant mice, and apparent differences between the transcriptional levels of the mip, pmp18D, and ompA genes in the samples taken during different time intervals of pregnancy were not observed (p > 0.05). The result indicated that the three bacterial genes, mip, pmp18D, and ompA, play a role as virulence factors in abortion and are differentially expressed in pregnant and nonpregnant animals. Inactivation of the genes is suggested to confirm the hypothesis. |
| first_indexed | 2024-12-10T21:12:44Z |
| format | Article |
| id | doaj.art-9e456e64327d4a2a9977aaf86e9a5187 |
| institution | Directory Open Access Journal |
| issn | 2544-4646 |
| language | English |
| last_indexed | 2024-12-10T21:12:44Z |
| publishDate | 2022-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-9e456e64327d4a2a9977aaf86e9a51872022-12-22T01:33:24ZengSciendoPolish Journal of Microbiology2544-46462022-03-0171111512110.33073/pjm-2022-014Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted EwesArif Eman Dhahir0Saeed Nahla Mohammad1Rachid Shwan Kamal2Dyary Hiewa Othman3Rashid Peshnyar M.A.4Department of Microbiology, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, IraqCharmo University, Sulaymaniyah, IraqDepartment of Basic Sciences, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, IraqKurdistan Institution for Strategic Studies and Scientific Research, Sulaymaniyah, IraqIn this manuscript, we report the proteins macrophage infectivity potentiator (mip, CAB080), major outer membrane protein (momp, CAB048), and polymorphic outer membrane protein (pmp18D, CAB776) that are expressed in different times of pregnancy in mice infected with Chlamydia abortus. Enzootic abortion of ewes (EAE) by C. abortus, an obligate intracellular pathogen, is a critical zoonotic disease-causing significant economic loss to livestock farming globally. This study was carried out for the detection and characterization of macrophage infectivity potentiator (mip, CAB080), major outer membrane protein (momp, CAB048), and polymorphic outer membrane protein (pmp18D, CAB776) using RT-qPCR. These proteins are believed to be expressed as virulence factors in C. abortus isolated from aborted ewes. BALB/c mice (pregnant and nonpregnant) were used as an animal model to be injected intraperitoneally with C. abortus culture in Vero cells since the endometrial lymphoid tissues of these animals resembles that of ewes. Also, the short duration of pregnancy in mice makes them a suitable animal model for obstetric studies. Tissue samples were taken from the mice after 10, 15, and 20 days of pregnancy to compare the expression of the genes mip, pmp18D, and ompA. Transcription level was quantified using RT-qPCR, the GAPDH transcription quantification, as a normalization signal. Abortion occurred in pregnant mice, and apparent differences between the transcriptional levels of the mip, pmp18D, and ompA genes in the samples taken during different time intervals of pregnancy were not observed (p > 0.05). The result indicated that the three bacterial genes, mip, pmp18D, and ompA, play a role as virulence factors in abortion and are differentially expressed in pregnant and nonpregnant animals. Inactivation of the genes is suggested to confirm the hypothesis.https://doi.org/10.33073/pjm-2022-014chlamydia abortusrt-qpcrmacrophage infectivity potentiator (mip)polymorphic membrane protein (pmp)major outer membrane protein (momp) |
| spellingShingle | Arif Eman Dhahir Saeed Nahla Mohammad Rachid Shwan Kamal Dyary Hiewa Othman Rashid Peshnyar M.A. Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes Polish Journal of Microbiology chlamydia abortus rt-qpcr macrophage infectivity potentiator (mip) polymorphic membrane protein (pmp) major outer membrane protein (momp) |
| title | Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes |
| title_full | Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes |
| title_fullStr | Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes |
| title_full_unstemmed | Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes |
| title_short | Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes |
| title_sort | expression level of the mip pmp18d and ompa genes in chlamydia abortus isolated from aborted ewes |
| topic | chlamydia abortus rt-qpcr macrophage infectivity potentiator (mip) polymorphic membrane protein (pmp) major outer membrane protein (momp) |
| url | https://doi.org/10.33073/pjm-2022-014 |
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