Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging
Abstract Soybean seeds contain phytochemicals such as polyamines and isoflavones, which have been identified as functional components mediating health benefits in association with the consumption of soy foods. While a clear picture of the spatial distribution of these components within the seed is l...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.1356 |
| _version_ | 1797221365840871424 |
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| author | Tatsuya Sagara Dhaka Ram Bhandari Bernhard Spengler Johann Vollmann |
| author_facet | Tatsuya Sagara Dhaka Ram Bhandari Bernhard Spengler Johann Vollmann |
| author_sort | Tatsuya Sagara |
| collection | DOAJ |
| description | Abstract Soybean seeds contain phytochemicals such as polyamines and isoflavones, which have been identified as functional components mediating health benefits in association with the consumption of soy foods. While a clear picture of the spatial distribution of these components within the seed is lacking, such information would be important to enhance or reduce their concentration in respective foods through processing. Thus, the objective of the present study was to visualize the most relevant components with respect to their distribution in soybean seeds. Mature soybean seeds were subject to atmospheric‐pressure scanning‐microprobe matrix‐assisted laser desorption/ionization (AP‐SMALDI) combined with a Fourier‐transform orbital trapping mass spectrometer to generate high‐resolution chemical images of phytochemical distribution. Based on seed cross sections, differential distributions of functional components were found between soybean cotyledon and germ (shoot, hypocotyl, root) regions. Spermidine and spermine were present in higher concentrations in the germ rather than in cotyledons with highest concentrations in root and shoot meristem tissues. Differential concentrations of spermidine and other components between the germ and cotyledon regions were confirmed by seed fractioning. In contrast to polyamines spermidine and spermine, the different types of daidzein, glycitein, and genistein isoflavones were all visualized in root parenchyma tissue exclusively. Overall, mass spectrometry imaging of soybean seeds revealed clear insights into the differential distribution of functional phytochemicals. Based on their distribution and depending on specific needs, spermidine and isoflavones can either be enriched or reduced during food processing by separating cotyledon and germ fractions. |
| first_indexed | 2024-04-24T13:04:17Z |
| format | Article |
| id | doaj.art-9e46ab788c35426eb664ef3154128e32 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-24T13:04:17Z |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-9e46ab788c35426eb664ef3154128e322024-04-05T09:16:03ZengWileyFood Science & Nutrition2048-71772020-01-018167568210.1002/fsn3.1356Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imagingTatsuya Sagara0Dhaka Ram Bhandari1Bernhard Spengler2Johann Vollmann3Department of Crop Sciences University of Natural Resources and Life Sciences Vienna (BOKU) Tulln an der Donau AustriaInstitute of Inorganic and Analytical Chemistry Justus Liebig University Giessen Giessen GermanyInstitute of Inorganic and Analytical Chemistry Justus Liebig University Giessen Giessen GermanyDepartment of Crop Sciences University of Natural Resources and Life Sciences Vienna (BOKU) Tulln an der Donau AustriaAbstract Soybean seeds contain phytochemicals such as polyamines and isoflavones, which have been identified as functional components mediating health benefits in association with the consumption of soy foods. While a clear picture of the spatial distribution of these components within the seed is lacking, such information would be important to enhance or reduce their concentration in respective foods through processing. Thus, the objective of the present study was to visualize the most relevant components with respect to their distribution in soybean seeds. Mature soybean seeds were subject to atmospheric‐pressure scanning‐microprobe matrix‐assisted laser desorption/ionization (AP‐SMALDI) combined with a Fourier‐transform orbital trapping mass spectrometer to generate high‐resolution chemical images of phytochemical distribution. Based on seed cross sections, differential distributions of functional components were found between soybean cotyledon and germ (shoot, hypocotyl, root) regions. Spermidine and spermine were present in higher concentrations in the germ rather than in cotyledons with highest concentrations in root and shoot meristem tissues. Differential concentrations of spermidine and other components between the germ and cotyledon regions were confirmed by seed fractioning. In contrast to polyamines spermidine and spermine, the different types of daidzein, glycitein, and genistein isoflavones were all visualized in root parenchyma tissue exclusively. Overall, mass spectrometry imaging of soybean seeds revealed clear insights into the differential distribution of functional phytochemicals. Based on their distribution and depending on specific needs, spermidine and isoflavones can either be enriched or reduced during food processing by separating cotyledon and germ fractions.https://doi.org/10.1002/fsn3.1356Isoflavonesmass spectrometry imagingpolyaminessoybeanspermidine |
| spellingShingle | Tatsuya Sagara Dhaka Ram Bhandari Bernhard Spengler Johann Vollmann Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging Food Science & Nutrition Isoflavones mass spectrometry imaging polyamines soybean spermidine |
| title | Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging |
| title_full | Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging |
| title_fullStr | Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging |
| title_full_unstemmed | Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging |
| title_short | Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging |
| title_sort | spermidine and other functional phytochemicals in soybean seeds spatial distribution as visualized by mass spectrometry imaging |
| topic | Isoflavones mass spectrometry imaging polyamines soybean spermidine |
| url | https://doi.org/10.1002/fsn3.1356 |
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